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Transcriptomic, proteomic and metabolomic analysis of UV-B signaling in maize.

Casati P, Campi M, Morrow DJ, Fernandes JF, Walbot V - BMC Genomics (2011)

Bottom Line: There were significant transcriptome, proteomic, and metabolomic changes under all conditions studied in both shielded and irradiated organs.A dramatic decrease in transcript diversity in irradiated and shielded leaves occurs between 0 h and 1 h, demonstrating the susceptibility of plants to short term UV-B spikes as during ozone depletion.Immature maize ears are highly responsive to canopy leaf exposure to UV-B.

View Article: PubMed Central - HTML - PubMed

Affiliation: Centro de Estudios Fotosintéticos y Bioquímicos, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Suipacha 531, 2000 Rosario, Argentina.

ABSTRACT

Background: Under normal solar fluence, UV-B damages macromolecules, but it also elicits physiological acclimation and developmental changes in plants. Excess UV-B decreases crop yield. Using a treatment twice solar fluence, we focus on discovering signals produced in UV-B-irradiated maize leaves that translate to systemic changes in shielded leaves and immature ears.

Results: Using transcriptome and proteomic profiling, we tracked the kinetics of transcript and protein alterations in exposed and shielded organs over 6 h. In parallel, metabolic profiling identified candidate signaling molecules based on rapid increase in irradiated leaves and increased levels in shielded organs; pathways associated with the synthesis, sequestration, or degradation of some of these potential signal molecules were UV-B-responsive. Exposure of just the top leaf substantially alters the transcriptomes of both irradiated and shielded organs, with greater changes as additional leaves are irradiated. Some phenylpropanoid pathway genes are expressed only in irradiated leaves, reflected in accumulation of pathway sunscreen molecules. Most protein changes detected occur quickly: approximately 92% of the proteins in leaves and 73% in immature ears changed after 4 h UV-B were altered by a 1 h UV-B treatment.

Conclusions: There were significant transcriptome, proteomic, and metabolomic changes under all conditions studied in both shielded and irradiated organs. A dramatic decrease in transcript diversity in irradiated and shielded leaves occurs between 0 h and 1 h, demonstrating the susceptibility of plants to short term UV-B spikes as during ozone depletion. Immature maize ears are highly responsive to canopy leaf exposure to UV-B.

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Related in: MedlinePlus

Transcriptome size in samples varying the canopy exposed and after different UV-B duration. (a and b) Transcriptome size in (a) whole irradiated (WPI), and non-irradiated plants (NI), and irradiated and shielded leaves from 1, 2 and 3 CLI plants, and (b) ears from CLI plants after 4 h of irradiation. (c and d) Transcriptome size in (c) irradiated and shielded leaves from 1, 2 and 3 CLI, and (d) immature ears from plants with two irradiated leaves for 1, 2, 4, and 6 h of UV-B.
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Figure 2: Transcriptome size in samples varying the canopy exposed and after different UV-B duration. (a and b) Transcriptome size in (a) whole irradiated (WPI), and non-irradiated plants (NI), and irradiated and shielded leaves from 1, 2 and 3 CLI plants, and (b) ears from CLI plants after 4 h of irradiation. (c and d) Transcriptome size in (c) irradiated and shielded leaves from 1, 2 and 3 CLI, and (d) immature ears from plants with two irradiated leaves for 1, 2, 4, and 6 h of UV-B.

Mentions: We first compared the transcriptome of the topmost leaves from plants exposed to 4 h UV-B (whole plant irradiation, WPI) to that from non-irradiated plants (NI): 203 transcripts decreased while 213 increased at least 2-fold (p < 0.05). These 416 transcripts represent ~2% of the leaf transcriptome (Figure 2a and 2b). At a 1.5-fold cut-off 714 transcripts are down-regulated and 862 up-regulated, summing to ~4% of the transcriptome.


Transcriptomic, proteomic and metabolomic analysis of UV-B signaling in maize.

Casati P, Campi M, Morrow DJ, Fernandes JF, Walbot V - BMC Genomics (2011)

Transcriptome size in samples varying the canopy exposed and after different UV-B duration. (a and b) Transcriptome size in (a) whole irradiated (WPI), and non-irradiated plants (NI), and irradiated and shielded leaves from 1, 2 and 3 CLI plants, and (b) ears from CLI plants after 4 h of irradiation. (c and d) Transcriptome size in (c) irradiated and shielded leaves from 1, 2 and 3 CLI, and (d) immature ears from plants with two irradiated leaves for 1, 2, 4, and 6 h of UV-B.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3141669&req=5

Figure 2: Transcriptome size in samples varying the canopy exposed and after different UV-B duration. (a and b) Transcriptome size in (a) whole irradiated (WPI), and non-irradiated plants (NI), and irradiated and shielded leaves from 1, 2 and 3 CLI plants, and (b) ears from CLI plants after 4 h of irradiation. (c and d) Transcriptome size in (c) irradiated and shielded leaves from 1, 2 and 3 CLI, and (d) immature ears from plants with two irradiated leaves for 1, 2, 4, and 6 h of UV-B.
Mentions: We first compared the transcriptome of the topmost leaves from plants exposed to 4 h UV-B (whole plant irradiation, WPI) to that from non-irradiated plants (NI): 203 transcripts decreased while 213 increased at least 2-fold (p < 0.05). These 416 transcripts represent ~2% of the leaf transcriptome (Figure 2a and 2b). At a 1.5-fold cut-off 714 transcripts are down-regulated and 862 up-regulated, summing to ~4% of the transcriptome.

Bottom Line: There were significant transcriptome, proteomic, and metabolomic changes under all conditions studied in both shielded and irradiated organs.A dramatic decrease in transcript diversity in irradiated and shielded leaves occurs between 0 h and 1 h, demonstrating the susceptibility of plants to short term UV-B spikes as during ozone depletion.Immature maize ears are highly responsive to canopy leaf exposure to UV-B.

View Article: PubMed Central - HTML - PubMed

Affiliation: Centro de Estudios Fotosintéticos y Bioquímicos, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Suipacha 531, 2000 Rosario, Argentina.

ABSTRACT

Background: Under normal solar fluence, UV-B damages macromolecules, but it also elicits physiological acclimation and developmental changes in plants. Excess UV-B decreases crop yield. Using a treatment twice solar fluence, we focus on discovering signals produced in UV-B-irradiated maize leaves that translate to systemic changes in shielded leaves and immature ears.

Results: Using transcriptome and proteomic profiling, we tracked the kinetics of transcript and protein alterations in exposed and shielded organs over 6 h. In parallel, metabolic profiling identified candidate signaling molecules based on rapid increase in irradiated leaves and increased levels in shielded organs; pathways associated with the synthesis, sequestration, or degradation of some of these potential signal molecules were UV-B-responsive. Exposure of just the top leaf substantially alters the transcriptomes of both irradiated and shielded organs, with greater changes as additional leaves are irradiated. Some phenylpropanoid pathway genes are expressed only in irradiated leaves, reflected in accumulation of pathway sunscreen molecules. Most protein changes detected occur quickly: approximately 92% of the proteins in leaves and 73% in immature ears changed after 4 h UV-B were altered by a 1 h UV-B treatment.

Conclusions: There were significant transcriptome, proteomic, and metabolomic changes under all conditions studied in both shielded and irradiated organs. A dramatic decrease in transcript diversity in irradiated and shielded leaves occurs between 0 h and 1 h, demonstrating the susceptibility of plants to short term UV-B spikes as during ozone depletion. Immature maize ears are highly responsive to canopy leaf exposure to UV-B.

Show MeSH
Related in: MedlinePlus