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Acute reduction of serum 8-iso-PGF2-alpha and advanced oxidation protein products in vivo by a polyphenol-rich beverage; a pilot clinical study with phytochemical and in vitro antioxidant characterization.

Nemzer BV, Rodriguez LC, Hammond L, Disilvestro R, Hunter JM, Pietrzkowski Z - Nutr J (2011)

Bottom Line: Both placebo and PRB treatment resulted in statistically significant increases in hydroxyl radical antioxidant capacity (HORAC) compared to baseline; PRB showed a higher percent change (55-75% versus 23-74% in placebo group), but the two groups did not differ significantly from each other.PRB produced statistically significant changes in several blood biomarkers related to antioxidant/anti-inflammatory effects.Future studies are justified to verify results and test for cumulative effects of repeated intakes of PRB.

View Article: PubMed Central - HTML - PubMed

Affiliation: FutureCeuticals, Inc. Momence, IL 60954, USA. bnemzer@vandrunen.com

ABSTRACT

Background: Measuring the effects of the acute intake of natural products on human biomarker concentrations, such as those related to oxidation and inflammation, can be an advantageous strategy for early clinical research on an ingredient or product.

Methods: 31 total healthy subjects were randomized in a double-blinded, placebo-controlled, acute pilot study with post-hoc subgroup analysis on 20 of the subjects. The study examined the effects of a single dose of a polyphenol-rich beverage (PRB), commercially marketed as "SoZo(®)", on serum anti-inflammatory and antioxidant markers. In addition, phytochemical analyses of PRB, and in vitro antioxidant capacity were also performed.

Results: At 1 hour post-intake, serum values for 8-iso-PGF2-alpha and advanced oxidation protein products decreased significantly by 40% and 39%, respectively. Additionally, there was a trend toward decreased C-reactive protein, and increased nitric oxide levels. Both placebo and PRB treatment resulted in statistically significant increases in hydroxyl radical antioxidant capacity (HORAC) compared to baseline; PRB showed a higher percent change (55-75% versus 23-74% in placebo group), but the two groups did not differ significantly from each other.

Conclusions: PRB produced statistically significant changes in several blood biomarkers related to antioxidant/anti-inflammatory effects. Future studies are justified to verify results and test for cumulative effects of repeated intakes of PRB. The study demonstrates the potential utility of acute biomarker measurements for evaluating antioxidant/anti-inflammatory effects of natural products.

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Effect of PBR on serum CRP levels; expressed as ng/mL + SEM.
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Figure 4: Effect of PBR on serum CRP levels; expressed as ng/mL + SEM.

Mentions: The characteristics of the study populations for the randomized, double blind, placebo-controlled acute clinical study were reasonably homogeneous are found in Table 2. Plasma levels of 8-iso-PGF2-alpha at T0 for all subjects are shown in Table 3. Subject number 22 was unable to complete all of the blood sample collections, and hence was not included. Subject 30's samples were disregarded due to hemolysis of blood. Biomarker analyses, performed on the 10 subjects from each group with the highest 8-iso-PGF2-alpha levels, are shown in Figures 1, 2, 3, 4 and 5; results are described below.


Acute reduction of serum 8-iso-PGF2-alpha and advanced oxidation protein products in vivo by a polyphenol-rich beverage; a pilot clinical study with phytochemical and in vitro antioxidant characterization.

Nemzer BV, Rodriguez LC, Hammond L, Disilvestro R, Hunter JM, Pietrzkowski Z - Nutr J (2011)

Effect of PBR on serum CRP levels; expressed as ng/mL + SEM.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3141640&req=5

Figure 4: Effect of PBR on serum CRP levels; expressed as ng/mL + SEM.
Mentions: The characteristics of the study populations for the randomized, double blind, placebo-controlled acute clinical study were reasonably homogeneous are found in Table 2. Plasma levels of 8-iso-PGF2-alpha at T0 for all subjects are shown in Table 3. Subject number 22 was unable to complete all of the blood sample collections, and hence was not included. Subject 30's samples were disregarded due to hemolysis of blood. Biomarker analyses, performed on the 10 subjects from each group with the highest 8-iso-PGF2-alpha levels, are shown in Figures 1, 2, 3, 4 and 5; results are described below.

Bottom Line: Both placebo and PRB treatment resulted in statistically significant increases in hydroxyl radical antioxidant capacity (HORAC) compared to baseline; PRB showed a higher percent change (55-75% versus 23-74% in placebo group), but the two groups did not differ significantly from each other.PRB produced statistically significant changes in several blood biomarkers related to antioxidant/anti-inflammatory effects.Future studies are justified to verify results and test for cumulative effects of repeated intakes of PRB.

View Article: PubMed Central - HTML - PubMed

Affiliation: FutureCeuticals, Inc. Momence, IL 60954, USA. bnemzer@vandrunen.com

ABSTRACT

Background: Measuring the effects of the acute intake of natural products on human biomarker concentrations, such as those related to oxidation and inflammation, can be an advantageous strategy for early clinical research on an ingredient or product.

Methods: 31 total healthy subjects were randomized in a double-blinded, placebo-controlled, acute pilot study with post-hoc subgroup analysis on 20 of the subjects. The study examined the effects of a single dose of a polyphenol-rich beverage (PRB), commercially marketed as "SoZo(®)", on serum anti-inflammatory and antioxidant markers. In addition, phytochemical analyses of PRB, and in vitro antioxidant capacity were also performed.

Results: At 1 hour post-intake, serum values for 8-iso-PGF2-alpha and advanced oxidation protein products decreased significantly by 40% and 39%, respectively. Additionally, there was a trend toward decreased C-reactive protein, and increased nitric oxide levels. Both placebo and PRB treatment resulted in statistically significant increases in hydroxyl radical antioxidant capacity (HORAC) compared to baseline; PRB showed a higher percent change (55-75% versus 23-74% in placebo group), but the two groups did not differ significantly from each other.

Conclusions: PRB produced statistically significant changes in several blood biomarkers related to antioxidant/anti-inflammatory effects. Future studies are justified to verify results and test for cumulative effects of repeated intakes of PRB. The study demonstrates the potential utility of acute biomarker measurements for evaluating antioxidant/anti-inflammatory effects of natural products.

Show MeSH