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Antiproliferation and cell apoptosis inducing bioactivities of constituents from Dysosma versipellis in PC3 and Bcap-37 cell lines.

Xu X, Gao X, Jin L, Bhadury PS, Yuan K, Hu D, Song B, Yang S - Cell Div (2011)

Bottom Line: The inhibitory activities of these compounds were investigated on tumor cells PC3, Bcap-37 and BGC-823 in vitro by MTT method, and the results showed that podophyllotoxone (PTO) and 4'-demethyldeoxypodophyllotoxin (DDPT) had potent inhibitory activities against the growth of human carcinoma cell lines.This study suggests that most of the compounds from the roots of D. versipellis could inhibit the growth of human carcinoma cells.In addition, PTO and DDPT could induce apoptosis of tumor cells.

View Article: PubMed Central - HTML - PubMed

Affiliation: State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Guiyang 550025, China. songbaoan22@yahoo.com.

ABSTRACT

Background: Recently, interest in phytochemicals from traditional Chinese medicinal herbs with the capability to inhibit cancer cells growth and proliferation has been growing rapidly due to their nontoxic nature. Dysosma versipellis as Bereridaceae plants is an endemic species in China, which has been proved to be an important Chinese herbal medicine because of its biological activity. However, systematic and comprehensive studies on the phytochemicals from Dysosma versipellis and their bioactivity are limited.

Results: Fifteen compounds were isolated and characterized from the roots of Dysosma versipellis, among which six compounds were isolated from this plant for the first time. The inhibitory activities of these compounds were investigated on tumor cells PC3, Bcap-37 and BGC-823 in vitro by MTT method, and the results showed that podophyllotoxone (PTO) and 4'-demethyldeoxypodophyllotoxin (DDPT) had potent inhibitory activities against the growth of human carcinoma cell lines. Subsequent fluorescence staining and flow cytometry analysis indicated that these two compounds could induce apoptosis in PC3 and Bcap-37 cells, and the apoptosis ratios reached the peak (12.0% and 14.1%) after 72 h of treatment at 20 μM, respectively.

Conclusions: This study suggests that most of the compounds from the roots of D. versipellis could inhibit the growth of human carcinoma cells. In addition, PTO and DDPT could induce apoptosis of tumor cells.

No MeSH data available.


Related in: MedlinePlus

Flow cytometry analysis for apoptosis inducing activities of PTO and DDPT on PC3 cells. The appearance of apoptosis cells was detected by flow cytometry using Annexin V/PI staining. In the figure, A, B and C: treated with HCPT (20 μM) for 24, 48 and72 h; D, E and F: treated with PTO (20 μM) for 24, 48 and 72 h; G, H and I: treated with DDPT (20 μM) for 24, 48 and 72 h.
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Figure 8: Flow cytometry analysis for apoptosis inducing activities of PTO and DDPT on PC3 cells. The appearance of apoptosis cells was detected by flow cytometry using Annexin V/PI staining. In the figure, A, B and C: treated with HCPT (20 μM) for 24, 48 and72 h; D, E and F: treated with PTO (20 μM) for 24, 48 and 72 h; G, H and I: treated with DDPT (20 μM) for 24, 48 and 72 h.

Mentions: In addition, the apoptosis ratios induced by PTO and DDPT caused apoptosis in tumor cells was quantitatively assessed by flow cytometry. In the early stages of apoptosis, phosphatidylserine (PS) was translocated from the inside of the cell membrane to the outside. Annexin V, a calcium dependent phospholipid-binding protein associated with a high affinity for phosphatidylserine, was used to detect early apoptotic cells. PI (Propidine Iodide) was a red fluorescent dye and stained cells that had lost membrane integrity. So, cells stained with FITC-annexin V and PI were discriminated necrotic cells (Q1, Annexin-/PI+), late apoptotic cells (Q2, Annexin+/PI+), intact cells (Q3, Annexin-/PI-) and early apoptotic cells (Q4, Annexin+/PI-). As shown in Figure 7, PTO and DDPT (20 μM) could induce apoptosis of PC3 cells, and highest apoptosis ratios, 12.0% and 14.1% for PTO and DDPT respectively, were obtained after 72 h of treatment at a concentration of 20 μM. Furthermore, as shown in Figure 8, the early (Q4) and late (Q2) apoptosis of PC3 cells which treated with PTO and DDPT increased gradually in a time-dependent manner. The late apoptotic ratio of cells increased to approximately 11.0% at 72 h after treatment of PTO (20 μM), which was close to that of positive control HCPT (11.8%). And the highest rate of early apoptosis was 7.0% when cells were treated with DDPT at the concentration of 20 μM for 72 h.


Antiproliferation and cell apoptosis inducing bioactivities of constituents from Dysosma versipellis in PC3 and Bcap-37 cell lines.

Xu X, Gao X, Jin L, Bhadury PS, Yuan K, Hu D, Song B, Yang S - Cell Div (2011)

Flow cytometry analysis for apoptosis inducing activities of PTO and DDPT on PC3 cells. The appearance of apoptosis cells was detected by flow cytometry using Annexin V/PI staining. In the figure, A, B and C: treated with HCPT (20 μM) for 24, 48 and72 h; D, E and F: treated with PTO (20 μM) for 24, 48 and 72 h; G, H and I: treated with DDPT (20 μM) for 24, 48 and 72 h.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3141614&req=5

Figure 8: Flow cytometry analysis for apoptosis inducing activities of PTO and DDPT on PC3 cells. The appearance of apoptosis cells was detected by flow cytometry using Annexin V/PI staining. In the figure, A, B and C: treated with HCPT (20 μM) for 24, 48 and72 h; D, E and F: treated with PTO (20 μM) for 24, 48 and 72 h; G, H and I: treated with DDPT (20 μM) for 24, 48 and 72 h.
Mentions: In addition, the apoptosis ratios induced by PTO and DDPT caused apoptosis in tumor cells was quantitatively assessed by flow cytometry. In the early stages of apoptosis, phosphatidylserine (PS) was translocated from the inside of the cell membrane to the outside. Annexin V, a calcium dependent phospholipid-binding protein associated with a high affinity for phosphatidylserine, was used to detect early apoptotic cells. PI (Propidine Iodide) was a red fluorescent dye and stained cells that had lost membrane integrity. So, cells stained with FITC-annexin V and PI were discriminated necrotic cells (Q1, Annexin-/PI+), late apoptotic cells (Q2, Annexin+/PI+), intact cells (Q3, Annexin-/PI-) and early apoptotic cells (Q4, Annexin+/PI-). As shown in Figure 7, PTO and DDPT (20 μM) could induce apoptosis of PC3 cells, and highest apoptosis ratios, 12.0% and 14.1% for PTO and DDPT respectively, were obtained after 72 h of treatment at a concentration of 20 μM. Furthermore, as shown in Figure 8, the early (Q4) and late (Q2) apoptosis of PC3 cells which treated with PTO and DDPT increased gradually in a time-dependent manner. The late apoptotic ratio of cells increased to approximately 11.0% at 72 h after treatment of PTO (20 μM), which was close to that of positive control HCPT (11.8%). And the highest rate of early apoptosis was 7.0% when cells were treated with DDPT at the concentration of 20 μM for 72 h.

Bottom Line: The inhibitory activities of these compounds were investigated on tumor cells PC3, Bcap-37 and BGC-823 in vitro by MTT method, and the results showed that podophyllotoxone (PTO) and 4'-demethyldeoxypodophyllotoxin (DDPT) had potent inhibitory activities against the growth of human carcinoma cell lines.This study suggests that most of the compounds from the roots of D. versipellis could inhibit the growth of human carcinoma cells.In addition, PTO and DDPT could induce apoptosis of tumor cells.

View Article: PubMed Central - HTML - PubMed

Affiliation: State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Guiyang 550025, China. songbaoan22@yahoo.com.

ABSTRACT

Background: Recently, interest in phytochemicals from traditional Chinese medicinal herbs with the capability to inhibit cancer cells growth and proliferation has been growing rapidly due to their nontoxic nature. Dysosma versipellis as Bereridaceae plants is an endemic species in China, which has been proved to be an important Chinese herbal medicine because of its biological activity. However, systematic and comprehensive studies on the phytochemicals from Dysosma versipellis and their bioactivity are limited.

Results: Fifteen compounds were isolated and characterized from the roots of Dysosma versipellis, among which six compounds were isolated from this plant for the first time. The inhibitory activities of these compounds were investigated on tumor cells PC3, Bcap-37 and BGC-823 in vitro by MTT method, and the results showed that podophyllotoxone (PTO) and 4'-demethyldeoxypodophyllotoxin (DDPT) had potent inhibitory activities against the growth of human carcinoma cell lines. Subsequent fluorescence staining and flow cytometry analysis indicated that these two compounds could induce apoptosis in PC3 and Bcap-37 cells, and the apoptosis ratios reached the peak (12.0% and 14.1%) after 72 h of treatment at 20 μM, respectively.

Conclusions: This study suggests that most of the compounds from the roots of D. versipellis could inhibit the growth of human carcinoma cells. In addition, PTO and DDPT could induce apoptosis of tumor cells.

No MeSH data available.


Related in: MedlinePlus