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Antiproliferation and cell apoptosis inducing bioactivities of constituents from Dysosma versipellis in PC3 and Bcap-37 cell lines.

Xu X, Gao X, Jin L, Bhadury PS, Yuan K, Hu D, Song B, Yang S - Cell Div (2011)

Bottom Line: The inhibitory activities of these compounds were investigated on tumor cells PC3, Bcap-37 and BGC-823 in vitro by MTT method, and the results showed that podophyllotoxone (PTO) and 4'-demethyldeoxypodophyllotoxin (DDPT) had potent inhibitory activities against the growth of human carcinoma cell lines.This study suggests that most of the compounds from the roots of D. versipellis could inhibit the growth of human carcinoma cells.In addition, PTO and DDPT could induce apoptosis of tumor cells.

View Article: PubMed Central - HTML - PubMed

Affiliation: State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Guiyang 550025, China. songbaoan22@yahoo.com.

ABSTRACT

Background: Recently, interest in phytochemicals from traditional Chinese medicinal herbs with the capability to inhibit cancer cells growth and proliferation has been growing rapidly due to their nontoxic nature. Dysosma versipellis as Bereridaceae plants is an endemic species in China, which has been proved to be an important Chinese herbal medicine because of its biological activity. However, systematic and comprehensive studies on the phytochemicals from Dysosma versipellis and their bioactivity are limited.

Results: Fifteen compounds were isolated and characterized from the roots of Dysosma versipellis, among which six compounds were isolated from this plant for the first time. The inhibitory activities of these compounds were investigated on tumor cells PC3, Bcap-37 and BGC-823 in vitro by MTT method, and the results showed that podophyllotoxone (PTO) and 4'-demethyldeoxypodophyllotoxin (DDPT) had potent inhibitory activities against the growth of human carcinoma cell lines. Subsequent fluorescence staining and flow cytometry analysis indicated that these two compounds could induce apoptosis in PC3 and Bcap-37 cells, and the apoptosis ratios reached the peak (12.0% and 14.1%) after 72 h of treatment at 20 μM, respectively.

Conclusions: This study suggests that most of the compounds from the roots of D. versipellis could inhibit the growth of human carcinoma cells. In addition, PTO and DDPT could induce apoptosis of tumor cells.

No MeSH data available.


Related in: MedlinePlus

Nuclei morphological changes during PTO and DDPT-induced apoptosis in PC3 and Bcap-37 cells detected by TUNEL assay. Tumor cells treated with PTO and DDPT (20 μM) were assayed by TUNEL and observed under light microscopy. For PC3 cells group, A: negative control (without treatment); C: positive control, treated with HCPT (20 μM) for 24 h; B and D: treated with PTO and DDPT (20 μM) for 24 h; For Bcap-37 cells group, A': negative control (without treatment); C': positive control, treated with HCPT (20 μM) for 24 h; B' and D': treated with PTO and DDPT (20 μM) for 24 h.
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Figure 6: Nuclei morphological changes during PTO and DDPT-induced apoptosis in PC3 and Bcap-37 cells detected by TUNEL assay. Tumor cells treated with PTO and DDPT (20 μM) were assayed by TUNEL and observed under light microscopy. For PC3 cells group, A: negative control (without treatment); C: positive control, treated with HCPT (20 μM) for 24 h; B and D: treated with PTO and DDPT (20 μM) for 24 h; For Bcap-37 cells group, A': negative control (without treatment); C': positive control, treated with HCPT (20 μM) for 24 h; B' and D': treated with PTO and DDPT (20 μM) for 24 h.

Mentions: TUNEL (Terminal deoxynucleotidyl Transferase Biotin-dUTP Nick End Labeling) is a popular method for identifying apoptotic cells in situ by detecting DNA fragmentation. Due to degradation of DNA that resulted from the activation of Ca/Mg dependent endonucleases in apoptotic cells, DNA cleavage occurred and led to breaking of strand within the DNA. These strand breaks of cleaved DNA could be identified by terminal deoxynucleotidyl transferase (TdT) that catalyzed the addition of biotin-dUTP. The biotin-labeled cleavage sites were then detected by reaction with streptavidin-HRP and visualized by DAB indicating a brown color. As shown in Figure 6, most nuclei were stained as a discernible brown in the treatment groups with HCPT (Figure 6C and 6C'), PTO (Figure 6B and 6B'), and DDPT (Figure 6D and 6D') compared with the control (Figure 6A and 6A').


Antiproliferation and cell apoptosis inducing bioactivities of constituents from Dysosma versipellis in PC3 and Bcap-37 cell lines.

Xu X, Gao X, Jin L, Bhadury PS, Yuan K, Hu D, Song B, Yang S - Cell Div (2011)

Nuclei morphological changes during PTO and DDPT-induced apoptosis in PC3 and Bcap-37 cells detected by TUNEL assay. Tumor cells treated with PTO and DDPT (20 μM) were assayed by TUNEL and observed under light microscopy. For PC3 cells group, A: negative control (without treatment); C: positive control, treated with HCPT (20 μM) for 24 h; B and D: treated with PTO and DDPT (20 μM) for 24 h; For Bcap-37 cells group, A': negative control (without treatment); C': positive control, treated with HCPT (20 μM) for 24 h; B' and D': treated with PTO and DDPT (20 μM) for 24 h.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3141614&req=5

Figure 6: Nuclei morphological changes during PTO and DDPT-induced apoptosis in PC3 and Bcap-37 cells detected by TUNEL assay. Tumor cells treated with PTO and DDPT (20 μM) were assayed by TUNEL and observed under light microscopy. For PC3 cells group, A: negative control (without treatment); C: positive control, treated with HCPT (20 μM) for 24 h; B and D: treated with PTO and DDPT (20 μM) for 24 h; For Bcap-37 cells group, A': negative control (without treatment); C': positive control, treated with HCPT (20 μM) for 24 h; B' and D': treated with PTO and DDPT (20 μM) for 24 h.
Mentions: TUNEL (Terminal deoxynucleotidyl Transferase Biotin-dUTP Nick End Labeling) is a popular method for identifying apoptotic cells in situ by detecting DNA fragmentation. Due to degradation of DNA that resulted from the activation of Ca/Mg dependent endonucleases in apoptotic cells, DNA cleavage occurred and led to breaking of strand within the DNA. These strand breaks of cleaved DNA could be identified by terminal deoxynucleotidyl transferase (TdT) that catalyzed the addition of biotin-dUTP. The biotin-labeled cleavage sites were then detected by reaction with streptavidin-HRP and visualized by DAB indicating a brown color. As shown in Figure 6, most nuclei were stained as a discernible brown in the treatment groups with HCPT (Figure 6C and 6C'), PTO (Figure 6B and 6B'), and DDPT (Figure 6D and 6D') compared with the control (Figure 6A and 6A').

Bottom Line: The inhibitory activities of these compounds were investigated on tumor cells PC3, Bcap-37 and BGC-823 in vitro by MTT method, and the results showed that podophyllotoxone (PTO) and 4'-demethyldeoxypodophyllotoxin (DDPT) had potent inhibitory activities against the growth of human carcinoma cell lines.This study suggests that most of the compounds from the roots of D. versipellis could inhibit the growth of human carcinoma cells.In addition, PTO and DDPT could induce apoptosis of tumor cells.

View Article: PubMed Central - HTML - PubMed

Affiliation: State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Guiyang 550025, China. songbaoan22@yahoo.com.

ABSTRACT

Background: Recently, interest in phytochemicals from traditional Chinese medicinal herbs with the capability to inhibit cancer cells growth and proliferation has been growing rapidly due to their nontoxic nature. Dysosma versipellis as Bereridaceae plants is an endemic species in China, which has been proved to be an important Chinese herbal medicine because of its biological activity. However, systematic and comprehensive studies on the phytochemicals from Dysosma versipellis and their bioactivity are limited.

Results: Fifteen compounds were isolated and characterized from the roots of Dysosma versipellis, among which six compounds were isolated from this plant for the first time. The inhibitory activities of these compounds were investigated on tumor cells PC3, Bcap-37 and BGC-823 in vitro by MTT method, and the results showed that podophyllotoxone (PTO) and 4'-demethyldeoxypodophyllotoxin (DDPT) had potent inhibitory activities against the growth of human carcinoma cell lines. Subsequent fluorescence staining and flow cytometry analysis indicated that these two compounds could induce apoptosis in PC3 and Bcap-37 cells, and the apoptosis ratios reached the peak (12.0% and 14.1%) after 72 h of treatment at 20 μM, respectively.

Conclusions: This study suggests that most of the compounds from the roots of D. versipellis could inhibit the growth of human carcinoma cells. In addition, PTO and DDPT could induce apoptosis of tumor cells.

No MeSH data available.


Related in: MedlinePlus