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Antiproliferation and cell apoptosis inducing bioactivities of constituents from Dysosma versipellis in PC3 and Bcap-37 cell lines.

Xu X, Gao X, Jin L, Bhadury PS, Yuan K, Hu D, Song B, Yang S - Cell Div (2011)

Bottom Line: The inhibitory activities of these compounds were investigated on tumor cells PC3, Bcap-37 and BGC-823 in vitro by MTT method, and the results showed that podophyllotoxone (PTO) and 4'-demethyldeoxypodophyllotoxin (DDPT) had potent inhibitory activities against the growth of human carcinoma cell lines.This study suggests that most of the compounds from the roots of D. versipellis could inhibit the growth of human carcinoma cells.In addition, PTO and DDPT could induce apoptosis of tumor cells.

View Article: PubMed Central - HTML - PubMed

Affiliation: State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Guiyang 550025, China. songbaoan22@yahoo.com.

ABSTRACT

Background: Recently, interest in phytochemicals from traditional Chinese medicinal herbs with the capability to inhibit cancer cells growth and proliferation has been growing rapidly due to their nontoxic nature. Dysosma versipellis as Bereridaceae plants is an endemic species in China, which has been proved to be an important Chinese herbal medicine because of its biological activity. However, systematic and comprehensive studies on the phytochemicals from Dysosma versipellis and their bioactivity are limited.

Results: Fifteen compounds were isolated and characterized from the roots of Dysosma versipellis, among which six compounds were isolated from this plant for the first time. The inhibitory activities of these compounds were investigated on tumor cells PC3, Bcap-37 and BGC-823 in vitro by MTT method, and the results showed that podophyllotoxone (PTO) and 4'-demethyldeoxypodophyllotoxin (DDPT) had potent inhibitory activities against the growth of human carcinoma cell lines. Subsequent fluorescence staining and flow cytometry analysis indicated that these two compounds could induce apoptosis in PC3 and Bcap-37 cells, and the apoptosis ratios reached the peak (12.0% and 14.1%) after 72 h of treatment at 20 μM, respectively.

Conclusions: This study suggests that most of the compounds from the roots of D. versipellis could inhibit the growth of human carcinoma cells. In addition, PTO and DDPT could induce apoptosis of tumor cells.

No MeSH data available.


Related in: MedlinePlus

Effect of PTO on proliferation of tumor cells. After PC3, Bcap-37, BGC-823 and NIT3T3 cells were treated with PTO for 72 h in the concentrations varied from 2.5 to 60 μM, growth inhibition of those tumor cells was detected by MTT assay. Data are presented as means ± SD, n = 4.
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Figure 2: Effect of PTO on proliferation of tumor cells. After PC3, Bcap-37, BGC-823 and NIT3T3 cells were treated with PTO for 72 h in the concentrations varied from 2.5 to 60 μM, growth inhibition of those tumor cells was detected by MTT assay. Data are presented as means ± SD, n = 4.

Mentions: The potential effect of the extracts from D. versipellis was investigated on the viability of PC3, Bcap-37, BGC-823 and NIH3T3 cells using MTT assay at the concentration of 20 μM, with ADM (adriamycin) [39] being used as the positive control [40,41]. MTT [3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide] assay is a common method of measuring the proliferation of cells. The results are summarized in Table 1. It could be seen that PTO and DDPT possess potent activities against the three human cancer cell lines tested. The inhibitory ratios of PTO and DDPT at 72 h after treatment were 52.0% and 67.1% against PC3 cells, 42.1% and 56.6% on Bcap-37 cells, 47.9% and 60.7% on BGC-823 cells, and 43.7% and 59.8% on NIH 3T3 cells. Further experiments found that proliferation of these three carcinoma cells were significantly inhibited by PTO and DDPT in a concentration-dependent manner, as shown in Figures 2 and 3. The IC50 values of PTO on PC3, Bcap-37 and BGC-823 cells were (17.8±1.0) μM, (21.1±1.8) μM, (19±1.6) μM respectively, while for DDPT, the IC50 values were (10.6±1.5) μM, (13.2±0.5) μM, (11.5±0.6) μM, respectively, which were both lower than that on NIH 3T3 cells [(24.2±2.1) μM for PTO; (16.2±9.9) μM for DDPT]. The results showed that PTO and DDPT had more potent activities against PC3, Bcap-37 and BGC-823 cells than on NIH3T3 cells. Besides, the inhibitory effect on tumor cells of DDPT was stronger than that of PTO.


Antiproliferation and cell apoptosis inducing bioactivities of constituents from Dysosma versipellis in PC3 and Bcap-37 cell lines.

Xu X, Gao X, Jin L, Bhadury PS, Yuan K, Hu D, Song B, Yang S - Cell Div (2011)

Effect of PTO on proliferation of tumor cells. After PC3, Bcap-37, BGC-823 and NIT3T3 cells were treated with PTO for 72 h in the concentrations varied from 2.5 to 60 μM, growth inhibition of those tumor cells was detected by MTT assay. Data are presented as means ± SD, n = 4.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3141614&req=5

Figure 2: Effect of PTO on proliferation of tumor cells. After PC3, Bcap-37, BGC-823 and NIT3T3 cells were treated with PTO for 72 h in the concentrations varied from 2.5 to 60 μM, growth inhibition of those tumor cells was detected by MTT assay. Data are presented as means ± SD, n = 4.
Mentions: The potential effect of the extracts from D. versipellis was investigated on the viability of PC3, Bcap-37, BGC-823 and NIH3T3 cells using MTT assay at the concentration of 20 μM, with ADM (adriamycin) [39] being used as the positive control [40,41]. MTT [3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide] assay is a common method of measuring the proliferation of cells. The results are summarized in Table 1. It could be seen that PTO and DDPT possess potent activities against the three human cancer cell lines tested. The inhibitory ratios of PTO and DDPT at 72 h after treatment were 52.0% and 67.1% against PC3 cells, 42.1% and 56.6% on Bcap-37 cells, 47.9% and 60.7% on BGC-823 cells, and 43.7% and 59.8% on NIH 3T3 cells. Further experiments found that proliferation of these three carcinoma cells were significantly inhibited by PTO and DDPT in a concentration-dependent manner, as shown in Figures 2 and 3. The IC50 values of PTO on PC3, Bcap-37 and BGC-823 cells were (17.8±1.0) μM, (21.1±1.8) μM, (19±1.6) μM respectively, while for DDPT, the IC50 values were (10.6±1.5) μM, (13.2±0.5) μM, (11.5±0.6) μM, respectively, which were both lower than that on NIH 3T3 cells [(24.2±2.1) μM for PTO; (16.2±9.9) μM for DDPT]. The results showed that PTO and DDPT had more potent activities against PC3, Bcap-37 and BGC-823 cells than on NIH3T3 cells. Besides, the inhibitory effect on tumor cells of DDPT was stronger than that of PTO.

Bottom Line: The inhibitory activities of these compounds were investigated on tumor cells PC3, Bcap-37 and BGC-823 in vitro by MTT method, and the results showed that podophyllotoxone (PTO) and 4'-demethyldeoxypodophyllotoxin (DDPT) had potent inhibitory activities against the growth of human carcinoma cell lines.This study suggests that most of the compounds from the roots of D. versipellis could inhibit the growth of human carcinoma cells.In addition, PTO and DDPT could induce apoptosis of tumor cells.

View Article: PubMed Central - HTML - PubMed

Affiliation: State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Guiyang 550025, China. songbaoan22@yahoo.com.

ABSTRACT

Background: Recently, interest in phytochemicals from traditional Chinese medicinal herbs with the capability to inhibit cancer cells growth and proliferation has been growing rapidly due to their nontoxic nature. Dysosma versipellis as Bereridaceae plants is an endemic species in China, which has been proved to be an important Chinese herbal medicine because of its biological activity. However, systematic and comprehensive studies on the phytochemicals from Dysosma versipellis and their bioactivity are limited.

Results: Fifteen compounds were isolated and characterized from the roots of Dysosma versipellis, among which six compounds were isolated from this plant for the first time. The inhibitory activities of these compounds were investigated on tumor cells PC3, Bcap-37 and BGC-823 in vitro by MTT method, and the results showed that podophyllotoxone (PTO) and 4'-demethyldeoxypodophyllotoxin (DDPT) had potent inhibitory activities against the growth of human carcinoma cell lines. Subsequent fluorescence staining and flow cytometry analysis indicated that these two compounds could induce apoptosis in PC3 and Bcap-37 cells, and the apoptosis ratios reached the peak (12.0% and 14.1%) after 72 h of treatment at 20 μM, respectively.

Conclusions: This study suggests that most of the compounds from the roots of D. versipellis could inhibit the growth of human carcinoma cells. In addition, PTO and DDPT could induce apoptosis of tumor cells.

No MeSH data available.


Related in: MedlinePlus