Limits...
Antiproliferation and cell apoptosis inducing bioactivities of constituents from Dysosma versipellis in PC3 and Bcap-37 cell lines.

Xu X, Gao X, Jin L, Bhadury PS, Yuan K, Hu D, Song B, Yang S - Cell Div (2011)

Bottom Line: The inhibitory activities of these compounds were investigated on tumor cells PC3, Bcap-37 and BGC-823 in vitro by MTT method, and the results showed that podophyllotoxone (PTO) and 4'-demethyldeoxypodophyllotoxin (DDPT) had potent inhibitory activities against the growth of human carcinoma cell lines.This study suggests that most of the compounds from the roots of D. versipellis could inhibit the growth of human carcinoma cells.In addition, PTO and DDPT could induce apoptosis of tumor cells.

View Article: PubMed Central - HTML - PubMed

Affiliation: State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Guiyang 550025, China. songbaoan22@yahoo.com.

ABSTRACT

Background: Recently, interest in phytochemicals from traditional Chinese medicinal herbs with the capability to inhibit cancer cells growth and proliferation has been growing rapidly due to their nontoxic nature. Dysosma versipellis as Bereridaceae plants is an endemic species in China, which has been proved to be an important Chinese herbal medicine because of its biological activity. However, systematic and comprehensive studies on the phytochemicals from Dysosma versipellis and their bioactivity are limited.

Results: Fifteen compounds were isolated and characterized from the roots of Dysosma versipellis, among which six compounds were isolated from this plant for the first time. The inhibitory activities of these compounds were investigated on tumor cells PC3, Bcap-37 and BGC-823 in vitro by MTT method, and the results showed that podophyllotoxone (PTO) and 4'-demethyldeoxypodophyllotoxin (DDPT) had potent inhibitory activities against the growth of human carcinoma cell lines. Subsequent fluorescence staining and flow cytometry analysis indicated that these two compounds could induce apoptosis in PC3 and Bcap-37 cells, and the apoptosis ratios reached the peak (12.0% and 14.1%) after 72 h of treatment at 20 μM, respectively.

Conclusions: This study suggests that most of the compounds from the roots of D. versipellis could inhibit the growth of human carcinoma cells. In addition, PTO and DDPT could induce apoptosis of tumor cells.

No MeSH data available.


Related in: MedlinePlus

The structures of compounds 1-15. These compounds were obtained from the root of Dysosma versipellis and identified by spectroscopic analysis and physicochemical data.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC3141614&req=5

Figure 1: The structures of compounds 1-15. These compounds were obtained from the root of Dysosma versipellis and identified by spectroscopic analysis and physicochemical data.

Mentions: The root of D. versipellis collected from Guizhou province was studied and fifteen compounds were isolated from the ethanol extracts, which were identified based on their physicochemical as well as spectroscopic data as β-sitosterol (1), 4'-demethylpodophyllotoxin (2), kaempferol (3), picropodophyllotoxin-4-O-glucoside (4), cleistanthin-B (5), kaempferol-3-O-β-D-glucopyranoside (6), 4'-demethylpodo-phyllotoxin-4-O-glucoside (7), quercetin-3-O-β-D-glucopyranoside (8), icropodo- phyllotoxin-4-O-β-D-glucopyranosyl-(1→6)-β-D-glucopyranoside (9), quercetin (10), daucosterol (11), podophyllotoxone (12), vanillic acid (13), 4'-demethyldeoxypodo-phyllotoxin (14), sucrose (15), as shown in Figure 1. It can be seen that these compounds can be classified as two steroids (compounds 1 and 11), four flavonoids (compounds 3, 6, 8 and 10), seven lignans (compounds 2, 4, 5, 7, 9, 12 and 14), an organic acid (compound 13), and sucrose (15).


Antiproliferation and cell apoptosis inducing bioactivities of constituents from Dysosma versipellis in PC3 and Bcap-37 cell lines.

Xu X, Gao X, Jin L, Bhadury PS, Yuan K, Hu D, Song B, Yang S - Cell Div (2011)

The structures of compounds 1-15. These compounds were obtained from the root of Dysosma versipellis and identified by spectroscopic analysis and physicochemical data.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3141614&req=5

Figure 1: The structures of compounds 1-15. These compounds were obtained from the root of Dysosma versipellis and identified by spectroscopic analysis and physicochemical data.
Mentions: The root of D. versipellis collected from Guizhou province was studied and fifteen compounds were isolated from the ethanol extracts, which were identified based on their physicochemical as well as spectroscopic data as β-sitosterol (1), 4'-demethylpodophyllotoxin (2), kaempferol (3), picropodophyllotoxin-4-O-glucoside (4), cleistanthin-B (5), kaempferol-3-O-β-D-glucopyranoside (6), 4'-demethylpodo-phyllotoxin-4-O-glucoside (7), quercetin-3-O-β-D-glucopyranoside (8), icropodo- phyllotoxin-4-O-β-D-glucopyranosyl-(1→6)-β-D-glucopyranoside (9), quercetin (10), daucosterol (11), podophyllotoxone (12), vanillic acid (13), 4'-demethyldeoxypodo-phyllotoxin (14), sucrose (15), as shown in Figure 1. It can be seen that these compounds can be classified as two steroids (compounds 1 and 11), four flavonoids (compounds 3, 6, 8 and 10), seven lignans (compounds 2, 4, 5, 7, 9, 12 and 14), an organic acid (compound 13), and sucrose (15).

Bottom Line: The inhibitory activities of these compounds were investigated on tumor cells PC3, Bcap-37 and BGC-823 in vitro by MTT method, and the results showed that podophyllotoxone (PTO) and 4'-demethyldeoxypodophyllotoxin (DDPT) had potent inhibitory activities against the growth of human carcinoma cell lines.This study suggests that most of the compounds from the roots of D. versipellis could inhibit the growth of human carcinoma cells.In addition, PTO and DDPT could induce apoptosis of tumor cells.

View Article: PubMed Central - HTML - PubMed

Affiliation: State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Guiyang 550025, China. songbaoan22@yahoo.com.

ABSTRACT

Background: Recently, interest in phytochemicals from traditional Chinese medicinal herbs with the capability to inhibit cancer cells growth and proliferation has been growing rapidly due to their nontoxic nature. Dysosma versipellis as Bereridaceae plants is an endemic species in China, which has been proved to be an important Chinese herbal medicine because of its biological activity. However, systematic and comprehensive studies on the phytochemicals from Dysosma versipellis and their bioactivity are limited.

Results: Fifteen compounds were isolated and characterized from the roots of Dysosma versipellis, among which six compounds were isolated from this plant for the first time. The inhibitory activities of these compounds were investigated on tumor cells PC3, Bcap-37 and BGC-823 in vitro by MTT method, and the results showed that podophyllotoxone (PTO) and 4'-demethyldeoxypodophyllotoxin (DDPT) had potent inhibitory activities against the growth of human carcinoma cell lines. Subsequent fluorescence staining and flow cytometry analysis indicated that these two compounds could induce apoptosis in PC3 and Bcap-37 cells, and the apoptosis ratios reached the peak (12.0% and 14.1%) after 72 h of treatment at 20 μM, respectively.

Conclusions: This study suggests that most of the compounds from the roots of D. versipellis could inhibit the growth of human carcinoma cells. In addition, PTO and DDPT could induce apoptosis of tumor cells.

No MeSH data available.


Related in: MedlinePlus