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Antibacterial activity of some selected medicinal plants of Pakistan.

Bibi Y, Nisa S, Chaudhary FM, Zia M - BMC Complement Altern Med (2011)

Bottom Line: The crude extract of P. integerrima and A. indica were active against all tested bacterial strains (12-23 mm zone of inhibition).Other four plant's crude extracts (Arisaema flavum, Debregeasia salicifolia, Carissa opaca, and Toona ciliata) were active against different bacterial strains.The crude extracts showed varying level of bactericidal activity.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Microbiology, Quaid-i-Azam University, Islamabad, Pakistan.

ABSTRACT

Background: Screening of the ethnobotenical plants is a pre-requisite to evaluate their therapeutic potential and it can lead to the isolation of new bioactive compounds.

Methods: The crude extracts and fractions of six medicinal important plants (Arisaema flavum, Debregeasia salicifolia, Carissa opaca, Pistacia integerrima, Aesculus indica, and Toona ciliata) were tested against three Gram positive and two Gram negative ATCC bacterial species using the agar well diffusion method.

Results: The crude extract of P. integerrima and A. indica were active against all tested bacterial strains (12-23 mm zone of inhibition). Other four plant's crude extracts (Arisaema flavum, Debregeasia salicifolia, Carissa opaca, and Toona ciliata) were active against different bacterial strains. The crude extracts showed varying level of bactericidal activity. The aqueous fractions of A. indica and P. integerrima crude extract showed maximum activity (19.66 and 16 mm, respectively) against B. subtilis, while the chloroform fractions of T. ciliata and D. salicifolia presented good antibacterial activities (13-17 mm zone of inhibition) against all the bacterial cultures tested.

Conclusion: The methanol fraction of Pistacia integerrima, chloroform fractions of Debregeasia salicifolia &Toona ciliata and aqueous fraction of Aesculus indica are suitable candidates for the development of novel antibacterial compounds.

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Comparative percent inhibition of thirty six extracts from six medicinal plants against different bacterial strains. Alphabets on bars represent LSD values at p < 0.05. Cr = Crude extract, Hx = Hexane fraction, Chf = Chloroform fraction, Etac = Ethyl acetate fraction, Moh = Methanol fraction, Aqu = Aqueous fraction
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Figure 2: Comparative percent inhibition of thirty six extracts from six medicinal plants against different bacterial strains. Alphabets on bars represent LSD values at p < 0.05. Cr = Crude extract, Hx = Hexane fraction, Chf = Chloroform fraction, Etac = Ethyl acetate fraction, Moh = Methanol fraction, Aqu = Aqueous fraction

Mentions: Figure 2 represents the comparative percent inhibition by all plants extracts. The crude extract of P. integerrima showed maximum inhibition of S. setubal (69.6%). Same extract also showed 65.5% inhibition of P. pickettii while, chloroform fraction of D. salicifolia showed good inhibition of P. pickettii (65.9%). A number of extracts showed good activities against many strains in a range 40-56% inhibition. Other scientists working in the same filed have also been reported on the variable antibacterial activity by plants extracts and their fractions. Such studies inspired the scientist to identify other bioactive compound through isolation [39-41]. Figure 2 also describes that in few cases crude extract exhibits moderate activity while their respective fractions showed pronounced activity. This might be due to distribution of active component in specific fraction depending upon its solubility nature. While, in few cases the crude extract is more active then fractions. The crude extract of P. integerrima exhibited more activity against all tested bacterial strains, as compared to its fractions. In such cases more then one active component might be present in crude extract which distributed into different fractions depending on their solubility. The clinical isolates were inhibited by plant extracts at varying degree. The extracts presenting ≥ 40 inhibition provides a good reason to use these plants for isolation of new antibacterial compound(s).


Antibacterial activity of some selected medicinal plants of Pakistan.

Bibi Y, Nisa S, Chaudhary FM, Zia M - BMC Complement Altern Med (2011)

Comparative percent inhibition of thirty six extracts from six medicinal plants against different bacterial strains. Alphabets on bars represent LSD values at p < 0.05. Cr = Crude extract, Hx = Hexane fraction, Chf = Chloroform fraction, Etac = Ethyl acetate fraction, Moh = Methanol fraction, Aqu = Aqueous fraction
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3141602&req=5

Figure 2: Comparative percent inhibition of thirty six extracts from six medicinal plants against different bacterial strains. Alphabets on bars represent LSD values at p < 0.05. Cr = Crude extract, Hx = Hexane fraction, Chf = Chloroform fraction, Etac = Ethyl acetate fraction, Moh = Methanol fraction, Aqu = Aqueous fraction
Mentions: Figure 2 represents the comparative percent inhibition by all plants extracts. The crude extract of P. integerrima showed maximum inhibition of S. setubal (69.6%). Same extract also showed 65.5% inhibition of P. pickettii while, chloroform fraction of D. salicifolia showed good inhibition of P. pickettii (65.9%). A number of extracts showed good activities against many strains in a range 40-56% inhibition. Other scientists working in the same filed have also been reported on the variable antibacterial activity by plants extracts and their fractions. Such studies inspired the scientist to identify other bioactive compound through isolation [39-41]. Figure 2 also describes that in few cases crude extract exhibits moderate activity while their respective fractions showed pronounced activity. This might be due to distribution of active component in specific fraction depending upon its solubility nature. While, in few cases the crude extract is more active then fractions. The crude extract of P. integerrima exhibited more activity against all tested bacterial strains, as compared to its fractions. In such cases more then one active component might be present in crude extract which distributed into different fractions depending on their solubility. The clinical isolates were inhibited by plant extracts at varying degree. The extracts presenting ≥ 40 inhibition provides a good reason to use these plants for isolation of new antibacterial compound(s).

Bottom Line: The crude extract of P. integerrima and A. indica were active against all tested bacterial strains (12-23 mm zone of inhibition).Other four plant's crude extracts (Arisaema flavum, Debregeasia salicifolia, Carissa opaca, and Toona ciliata) were active against different bacterial strains.The crude extracts showed varying level of bactericidal activity.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Microbiology, Quaid-i-Azam University, Islamabad, Pakistan.

ABSTRACT

Background: Screening of the ethnobotenical plants is a pre-requisite to evaluate their therapeutic potential and it can lead to the isolation of new bioactive compounds.

Methods: The crude extracts and fractions of six medicinal important plants (Arisaema flavum, Debregeasia salicifolia, Carissa opaca, Pistacia integerrima, Aesculus indica, and Toona ciliata) were tested against three Gram positive and two Gram negative ATCC bacterial species using the agar well diffusion method.

Results: The crude extract of P. integerrima and A. indica were active against all tested bacterial strains (12-23 mm zone of inhibition). Other four plant's crude extracts (Arisaema flavum, Debregeasia salicifolia, Carissa opaca, and Toona ciliata) were active against different bacterial strains. The crude extracts showed varying level of bactericidal activity. The aqueous fractions of A. indica and P. integerrima crude extract showed maximum activity (19.66 and 16 mm, respectively) against B. subtilis, while the chloroform fractions of T. ciliata and D. salicifolia presented good antibacterial activities (13-17 mm zone of inhibition) against all the bacterial cultures tested.

Conclusion: The methanol fraction of Pistacia integerrima, chloroform fractions of Debregeasia salicifolia &Toona ciliata and aqueous fraction of Aesculus indica are suitable candidates for the development of novel antibacterial compounds.

Show MeSH
Related in: MedlinePlus