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Protective effects of L-carnitine on intestinal ischemia/reperfusion injury in a rat model.

Yuan Y, Guo H, Zhang Y, Zhou D, Gan P, Liang DM, Chen JY - J Clin Med Res (2011)

Bottom Line: The degree of pathological impairment in small intestine was lighter in IR+L than IR group (P < 0.05).The L-carnitine pretreatment has a positive effect on reducing levels of BT, on inhibiting secretion of proinflammatory cytokines, and on lessening intestinal mucosa injury during small intestinal IR injury.L-carnitine; Ischemia/reperfusion injury; Intestine.

View Article: PubMed Central - PubMed

Affiliation: Department of Emergency, The Second Affiliated Hospital of Kunming Medical College, Kunming, Yunnan 650101, China.

ABSTRACT

Background: Ischemia/reperfusion (IR) injury of the intestine is a major problem in abdominal pathological condition and is associated with a high morbidity and mortality. The purpose of the study is to determine whether the L-carnitine can prevent the harmful effects of small intestinal IR injury in rats.

Methods: Thirty Sprague-Dawley rats were randomly divided into three groups. Sham operated group (S), for shamoperated, the IR group for rats submitted to 45-minute of intestinal ischemia and 2-hour reperfusion, and IR+L group for those IR group treated with L-carnitine before reperfusion. All the rats were given EmGFP labelled E. coli DH5α through gavage 2-hour before the operative procedure. Afterwards the bacterial translocation (BT) from mesenteric lymph nodes (MLN), liver, spleen, lung and portal vein blood were detected. And the colony forming units/g (CFU/g) were counted. The TNF-α, IL-1β, IL-6, and IL-10 in serum were measured by ELISA. The morphometric study was measured by Chius classification.

Results: The levels of BT were higher in the IR group than IR+L group (P < 0.05). The E. coli DH5α was hardly detected in the S group. The IR+L rats had enhancement of IL-10 and suppressed production of serum TNF-α, IL-1β and IL-6, compared to IR group rats (P < 0.05). The degree of pathological impairment in small intestine was lighter in IR+L than IR group (P < 0.05).

Conclusions: The L-carnitine pretreatment has a positive effect on reducing levels of BT, on inhibiting secretion of proinflammatory cytokines, and on lessening intestinal mucosa injury during small intestinal IR injury.

Keywords: L-carnitine; Ischemia/reperfusion injury; Intestine.

No MeSH data available.


Related in: MedlinePlus

Normal intestinal mucosa in the S group rats (Grade 0) (H&E, 100).
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Figure 2: Normal intestinal mucosa in the S group rats (Grade 0) (H&E, 100).

Mentions: The villus and glands were normal and inflammatory cell infiltration was not observed in mucosal epithelial layer in the sham operated group (Fig. 2). Multiple erosions, inflammatory cells and bleeding were observed in the IR group (Fig. 3). Lighter edema of mucosa villus and fewer infiltration of necrotic epithelial inflammatory cells neutrophil leukomonocyte were found in mucosa epithelial layer in IR+L group than the IR group (Fig. 4).


Protective effects of L-carnitine on intestinal ischemia/reperfusion injury in a rat model.

Yuan Y, Guo H, Zhang Y, Zhou D, Gan P, Liang DM, Chen JY - J Clin Med Res (2011)

Normal intestinal mucosa in the S group rats (Grade 0) (H&E, 100).
© Copyright Policy - open access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3140927&req=5

Figure 2: Normal intestinal mucosa in the S group rats (Grade 0) (H&E, 100).
Mentions: The villus and glands were normal and inflammatory cell infiltration was not observed in mucosal epithelial layer in the sham operated group (Fig. 2). Multiple erosions, inflammatory cells and bleeding were observed in the IR group (Fig. 3). Lighter edema of mucosa villus and fewer infiltration of necrotic epithelial inflammatory cells neutrophil leukomonocyte were found in mucosa epithelial layer in IR+L group than the IR group (Fig. 4).

Bottom Line: The degree of pathological impairment in small intestine was lighter in IR+L than IR group (P < 0.05).The L-carnitine pretreatment has a positive effect on reducing levels of BT, on inhibiting secretion of proinflammatory cytokines, and on lessening intestinal mucosa injury during small intestinal IR injury.L-carnitine; Ischemia/reperfusion injury; Intestine.

View Article: PubMed Central - PubMed

Affiliation: Department of Emergency, The Second Affiliated Hospital of Kunming Medical College, Kunming, Yunnan 650101, China.

ABSTRACT

Background: Ischemia/reperfusion (IR) injury of the intestine is a major problem in abdominal pathological condition and is associated with a high morbidity and mortality. The purpose of the study is to determine whether the L-carnitine can prevent the harmful effects of small intestinal IR injury in rats.

Methods: Thirty Sprague-Dawley rats were randomly divided into three groups. Sham operated group (S), for shamoperated, the IR group for rats submitted to 45-minute of intestinal ischemia and 2-hour reperfusion, and IR+L group for those IR group treated with L-carnitine before reperfusion. All the rats were given EmGFP labelled E. coli DH5α through gavage 2-hour before the operative procedure. Afterwards the bacterial translocation (BT) from mesenteric lymph nodes (MLN), liver, spleen, lung and portal vein blood were detected. And the colony forming units/g (CFU/g) were counted. The TNF-α, IL-1β, IL-6, and IL-10 in serum were measured by ELISA. The morphometric study was measured by Chius classification.

Results: The levels of BT were higher in the IR group than IR+L group (P < 0.05). The E. coli DH5α was hardly detected in the S group. The IR+L rats had enhancement of IL-10 and suppressed production of serum TNF-α, IL-1β and IL-6, compared to IR group rats (P < 0.05). The degree of pathological impairment in small intestine was lighter in IR+L than IR group (P < 0.05).

Conclusions: The L-carnitine pretreatment has a positive effect on reducing levels of BT, on inhibiting secretion of proinflammatory cytokines, and on lessening intestinal mucosa injury during small intestinal IR injury.

Keywords: L-carnitine; Ischemia/reperfusion injury; Intestine.

No MeSH data available.


Related in: MedlinePlus