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Oxalic acid, versatile peroxidase secretion and chelating ability of Bjerkandera fumosa in rich and limited culture conditions.

Grąz M, Jarosz-Wilkołazka A - World J. Microbiol. Biotechnol. (2011)

Bottom Line: Efficient ligninolytic systems of wood-degrading fungi include not only oxidizing enzymes, but also low-molecular-weight effectors.Higher activity of VP was determined in the nitrogen-limited media but greater concentration of oxalic acid was observed in the cultures of B. fumosa without nitrogen limitation.Oxalate decarboxylase was found to be responsible for regulation of oxalic acid concentration in the tested B. fumosa cultures.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry, Maria Curie-Sklodowska University, Akademicka 19, 20-033 Lublin, Poland.

ABSTRACT
Efficient ligninolytic systems of wood-degrading fungi include not only oxidizing enzymes, but also low-molecular-weight effectors. The ability of Bjerkandera fumosa to secrete oxalic acid and versatile peroxidase (VP) in nitrogen-rich and nitrogen-limited media was studied. Higher activity of VP was determined in the nitrogen-limited media but greater concentration of oxalic acid was observed in the cultures of B. fumosa without nitrogen limitation. Ferric ions chelating ability of Bjerkandera fumosa studied in ferric ions limited media was correlated with the increased level of oxalic acid. The presence of hydroxamate-type siderophores in B. fumosa media were also detected. Oxalate decarboxylase was found to be responsible for regulation of oxalic acid concentration in the tested B. fumosa cultures.

No MeSH data available.


Total ability to ferric ions chelation (% Usid) and concentration of oxalic acid during B. fumosa growth on N-rich BLM and N-rich BLMFe. Data points are the means and the standard deviations of three repetitions
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Fig2: Total ability to ferric ions chelation (% Usid) and concentration of oxalic acid during B. fumosa growth on N-rich BLM and N-rich BLMFe. Data points are the means and the standard deviations of three repetitions

Mentions: The nonspecific CAS assay was applied to investigate the ability of B. fumosa to the Fe3+ ions chelation and therefore the fungus was cultivated with ferric ions limitation (N-rich BLMFe) and without limitation (N-rich BLM). Total chelating ability of fungal cultures can be influencing by oxalate accumulation in growth media. To investigate this correlation, the level of oxalic acid was monitored in cultures of B. fumosa and distinct differences between tested (N-rich BLMFe) and control (N-rich BLM) cultures in chelation ability were observed (Fig. 2). In tested cultures the chelation ability defined as percentage of siderophore units (% Usid) was at the high, constant level during 21 days of growth (in the range between 71.6 and 84.6% Usid) and became reduced to 38% Usid on the 28th day of growing. In control cultures the tested parameter oscillated during the time of fungal growth between 9.2 and 54% Usid with the exception on 7th day of cultivation when it reached value of 77.5% Usid. The highest differences in ferric ions chelating ability between tested and control cultures of B. fumosa were detected on 14th day of fungal growth and were nine times greater in tested cultures with Fe3+-limitation (81.4% Usid) than in controls without Fe3+-limitation (9.2% Usid). This higher rate of CAS reaction was correlated with enhanced oxalate secretion in Fe3+-limited cultures (N-rich BLMFe) (Fig. 2). Oxalic acid in Fe3+-limitation conditions remained at higher level in comparison with control cultures during entire period of cultivation.Fig. 2


Oxalic acid, versatile peroxidase secretion and chelating ability of Bjerkandera fumosa in rich and limited culture conditions.

Grąz M, Jarosz-Wilkołazka A - World J. Microbiol. Biotechnol. (2011)

Total ability to ferric ions chelation (% Usid) and concentration of oxalic acid during B. fumosa growth on N-rich BLM and N-rich BLMFe. Data points are the means and the standard deviations of three repetitions
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3140919&req=5

Fig2: Total ability to ferric ions chelation (% Usid) and concentration of oxalic acid during B. fumosa growth on N-rich BLM and N-rich BLMFe. Data points are the means and the standard deviations of three repetitions
Mentions: The nonspecific CAS assay was applied to investigate the ability of B. fumosa to the Fe3+ ions chelation and therefore the fungus was cultivated with ferric ions limitation (N-rich BLMFe) and without limitation (N-rich BLM). Total chelating ability of fungal cultures can be influencing by oxalate accumulation in growth media. To investigate this correlation, the level of oxalic acid was monitored in cultures of B. fumosa and distinct differences between tested (N-rich BLMFe) and control (N-rich BLM) cultures in chelation ability were observed (Fig. 2). In tested cultures the chelation ability defined as percentage of siderophore units (% Usid) was at the high, constant level during 21 days of growth (in the range between 71.6 and 84.6% Usid) and became reduced to 38% Usid on the 28th day of growing. In control cultures the tested parameter oscillated during the time of fungal growth between 9.2 and 54% Usid with the exception on 7th day of cultivation when it reached value of 77.5% Usid. The highest differences in ferric ions chelating ability between tested and control cultures of B. fumosa were detected on 14th day of fungal growth and were nine times greater in tested cultures with Fe3+-limitation (81.4% Usid) than in controls without Fe3+-limitation (9.2% Usid). This higher rate of CAS reaction was correlated with enhanced oxalate secretion in Fe3+-limited cultures (N-rich BLMFe) (Fig. 2). Oxalic acid in Fe3+-limitation conditions remained at higher level in comparison with control cultures during entire period of cultivation.Fig. 2

Bottom Line: Efficient ligninolytic systems of wood-degrading fungi include not only oxidizing enzymes, but also low-molecular-weight effectors.Higher activity of VP was determined in the nitrogen-limited media but greater concentration of oxalic acid was observed in the cultures of B. fumosa without nitrogen limitation.Oxalate decarboxylase was found to be responsible for regulation of oxalic acid concentration in the tested B. fumosa cultures.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry, Maria Curie-Sklodowska University, Akademicka 19, 20-033 Lublin, Poland.

ABSTRACT
Efficient ligninolytic systems of wood-degrading fungi include not only oxidizing enzymes, but also low-molecular-weight effectors. The ability of Bjerkandera fumosa to secrete oxalic acid and versatile peroxidase (VP) in nitrogen-rich and nitrogen-limited media was studied. Higher activity of VP was determined in the nitrogen-limited media but greater concentration of oxalic acid was observed in the cultures of B. fumosa without nitrogen limitation. Ferric ions chelating ability of Bjerkandera fumosa studied in ferric ions limited media was correlated with the increased level of oxalic acid. The presence of hydroxamate-type siderophores in B. fumosa media were also detected. Oxalate decarboxylase was found to be responsible for regulation of oxalic acid concentration in the tested B. fumosa cultures.

No MeSH data available.