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Traditional Herbal Medicine, Rikkunshito, Induces HSP60 and Enhances Cytoprotection of Small Intestinal Mucosal Cells as a Nontoxic Chaperone Inducer.

Tamaki K, Otaka M, Shibuya T, Sakamoto N, Yamamoto S, Odashima M, Itoh H, Watanabe S - Evid Based Complement Alternat Med (2011)

Bottom Line: HSP60 was significantly induced by TJ-43.Cell necrosis and apoptosis were significantly suppressed in IEC-6 cells pretreated by TJ-43 with overexpression of HSP60.Our results suggested that HSP60 induced by TJ-43 might play an important role in protecting small intestinal epithelial cells from apoptosis and necrosis in vitro.

View Article: PubMed Central - PubMed

Affiliation: Department of Gastroenterology, Juntendo University School of Medicine, 2-1-1 Hongo, Bunkyo-Ku, Tokyo 113-8421, Japan.

ABSTRACT
Increasing incidence of small intestinal ulcers associated with nonsteroidal anti-inflammatory drugs (NSAIDs) has become a topic with recent advances of endoscopic technology. However, the pathogenesis and therapy are not fully understood. The aim of this study is to examine the effect of Rikkunshito (TJ-43), a traditional herbal medicine, on expression of HSP60 and cytoprotective ability in small intestinal cell line (IEC-6). Effect of TJ-43 on HSP60 expression in IEC-6 cells was evaluated by immunoblot analysis. The effect of TJ-43 on cytoprotective abilities of IEC-6 cells against hydrogen peroxide or indomethacin was studied by MTT assay, LDH-release assay, caspase-8 activity, and TUNEL. HSP60 was significantly induced by TJ-43. Cell necrosis and apoptosis were significantly suppressed in IEC-6 cells pretreated by TJ-43 with overexpression of HSP60. Our results suggested that HSP60 induced by TJ-43 might play an important role in protecting small intestinal epithelial cells from apoptosis and necrosis in vitro.

No MeSH data available.


Related in: MedlinePlus

Western blot analysis. TJ-43 significantly induced the expression levels of HSP60 in IEC-6 cells **P < 0.01 compared with control cells.
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fig1: Western blot analysis. TJ-43 significantly induced the expression levels of HSP60 in IEC-6 cells **P < 0.01 compared with control cells.

Mentions: As shown in Figure 1, TJ-43 significantly induced HSP60 expression (approximately 2-fold) compared with that in IEC-6 cells determined by immunoblotting with quantification by densitometric data normalized by internal control protein (GAPDH) (P < 0.01).


Traditional Herbal Medicine, Rikkunshito, Induces HSP60 and Enhances Cytoprotection of Small Intestinal Mucosal Cells as a Nontoxic Chaperone Inducer.

Tamaki K, Otaka M, Shibuya T, Sakamoto N, Yamamoto S, Odashima M, Itoh H, Watanabe S - Evid Based Complement Alternat Med (2011)

Western blot analysis. TJ-43 significantly induced the expression levels of HSP60 in IEC-6 cells **P < 0.01 compared with control cells.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3140780&req=5

fig1: Western blot analysis. TJ-43 significantly induced the expression levels of HSP60 in IEC-6 cells **P < 0.01 compared with control cells.
Mentions: As shown in Figure 1, TJ-43 significantly induced HSP60 expression (approximately 2-fold) compared with that in IEC-6 cells determined by immunoblotting with quantification by densitometric data normalized by internal control protein (GAPDH) (P < 0.01).

Bottom Line: HSP60 was significantly induced by TJ-43.Cell necrosis and apoptosis were significantly suppressed in IEC-6 cells pretreated by TJ-43 with overexpression of HSP60.Our results suggested that HSP60 induced by TJ-43 might play an important role in protecting small intestinal epithelial cells from apoptosis and necrosis in vitro.

View Article: PubMed Central - PubMed

Affiliation: Department of Gastroenterology, Juntendo University School of Medicine, 2-1-1 Hongo, Bunkyo-Ku, Tokyo 113-8421, Japan.

ABSTRACT
Increasing incidence of small intestinal ulcers associated with nonsteroidal anti-inflammatory drugs (NSAIDs) has become a topic with recent advances of endoscopic technology. However, the pathogenesis and therapy are not fully understood. The aim of this study is to examine the effect of Rikkunshito (TJ-43), a traditional herbal medicine, on expression of HSP60 and cytoprotective ability in small intestinal cell line (IEC-6). Effect of TJ-43 on HSP60 expression in IEC-6 cells was evaluated by immunoblot analysis. The effect of TJ-43 on cytoprotective abilities of IEC-6 cells against hydrogen peroxide or indomethacin was studied by MTT assay, LDH-release assay, caspase-8 activity, and TUNEL. HSP60 was significantly induced by TJ-43. Cell necrosis and apoptosis were significantly suppressed in IEC-6 cells pretreated by TJ-43 with overexpression of HSP60. Our results suggested that HSP60 induced by TJ-43 might play an important role in protecting small intestinal epithelial cells from apoptosis and necrosis in vitro.

No MeSH data available.


Related in: MedlinePlus