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KLF4 Expression Correlates with the Degree of Differentiation in Colorectal Cancer.

Hu R, Zuo Y, Zuo L, Liu C, Zhang S, Wu Q, Zhou Q, Gui S, Wei W, Wang Y - Gut Liver (2011)

Bottom Line: We tried to find whether KLF4 expression is associated with the progression and differentiation of colorectal cancer.Our results clearly indicate that KLF4 protein expression significantly correlates with the degree of differentiation in colorectal cancers (p<0.05).Downregulation of KLF4 expression may lead to more poorly differentiated tumors.

View Article: PubMed Central - PubMed

Affiliation: Institute of Clinical Pharmacology and Key Laboratory of Anti-Inflammatory and Immune Pharmacology, Hefei, China.

ABSTRACT

Background/aims: Krüppel-like factor 4 (KLF4) is an epithelial-specific transcription factor primarily expressed in the gastrointestinal tract that mediates growth arrest in the colonic epithelium. We tried to find whether KLF4 expression is associated with the progression and differentiation of colorectal cancer.

Methods: We detected KLF4 expression in 109 colorectal specimens (40 normal appearing mucosa, 7 adenomas, and 62 carcinomas) by immunohistochemistry using a tissue microarray. Western blot and RT-PCR analyses were also performed.

Results: The upregulation of KLF4 expression in carcinoma tissue was statistically significant (p<0.05) when compared to normal appearing mucosa. The negative and weak positive staining rates in normal appearing mucosa, adenoma, and carcinoma were 42.5%, 71.4%, and 82.3%, respectively, indicating a decreased degree of KLF4 expression over the course of progressive transformation of normal cells into malignant derivatives. KLF4 protein levels showed no correlation with sex, age, or metastatic state (p>0.05), while KLF4 protein expression correlated with the diagnostic stage (p<0.05). Furthermore, strong KLF4 staining was detected in 22.9% (11/48) and 0% (0/14) of well/moderately and poorly differentiated colorectal cancers, respectively. Our results clearly indicate that KLF4 protein expression significantly correlates with the degree of differentiation in colorectal cancers (p<0.05). KLF4 expression in RKO cells is also upregulated by butyrate, an inducer of differentiation.

Conclusions: Downregulation of KLF4 expression may lead to more poorly differentiated tumors.

No MeSH data available.


Related in: MedlinePlus

Western bolt analysis of KLF4 protein expression in colorectal tissue. High KLF4 expression is detected in normal colorectal and well-differentiated tissues. Moreover, a downregulation of expression is observed in poorly differentiated colorectal cancer tissues. Lanes 1 and 3 show KLF4 expression in normal-appearing tissues, while the other lanes show expression in cancerous specimens.
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Figure 3: Western bolt analysis of KLF4 protein expression in colorectal tissue. High KLF4 expression is detected in normal colorectal and well-differentiated tissues. Moreover, a downregulation of expression is observed in poorly differentiated colorectal cancer tissues. Lanes 1 and 3 show KLF4 expression in normal-appearing tissues, while the other lanes show expression in cancerous specimens.

Mentions: As showed in Fig. 3, the expression of KLF4 protein was lower in colorectal tumor than that in normal-appearing colorectal mucosa.


KLF4 Expression Correlates with the Degree of Differentiation in Colorectal Cancer.

Hu R, Zuo Y, Zuo L, Liu C, Zhang S, Wu Q, Zhou Q, Gui S, Wei W, Wang Y - Gut Liver (2011)

Western bolt analysis of KLF4 protein expression in colorectal tissue. High KLF4 expression is detected in normal colorectal and well-differentiated tissues. Moreover, a downregulation of expression is observed in poorly differentiated colorectal cancer tissues. Lanes 1 and 3 show KLF4 expression in normal-appearing tissues, while the other lanes show expression in cancerous specimens.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3140659&req=5

Figure 3: Western bolt analysis of KLF4 protein expression in colorectal tissue. High KLF4 expression is detected in normal colorectal and well-differentiated tissues. Moreover, a downregulation of expression is observed in poorly differentiated colorectal cancer tissues. Lanes 1 and 3 show KLF4 expression in normal-appearing tissues, while the other lanes show expression in cancerous specimens.
Mentions: As showed in Fig. 3, the expression of KLF4 protein was lower in colorectal tumor than that in normal-appearing colorectal mucosa.

Bottom Line: We tried to find whether KLF4 expression is associated with the progression and differentiation of colorectal cancer.Our results clearly indicate that KLF4 protein expression significantly correlates with the degree of differentiation in colorectal cancers (p<0.05).Downregulation of KLF4 expression may lead to more poorly differentiated tumors.

View Article: PubMed Central - PubMed

Affiliation: Institute of Clinical Pharmacology and Key Laboratory of Anti-Inflammatory and Immune Pharmacology, Hefei, China.

ABSTRACT

Background/aims: Krüppel-like factor 4 (KLF4) is an epithelial-specific transcription factor primarily expressed in the gastrointestinal tract that mediates growth arrest in the colonic epithelium. We tried to find whether KLF4 expression is associated with the progression and differentiation of colorectal cancer.

Methods: We detected KLF4 expression in 109 colorectal specimens (40 normal appearing mucosa, 7 adenomas, and 62 carcinomas) by immunohistochemistry using a tissue microarray. Western blot and RT-PCR analyses were also performed.

Results: The upregulation of KLF4 expression in carcinoma tissue was statistically significant (p<0.05) when compared to normal appearing mucosa. The negative and weak positive staining rates in normal appearing mucosa, adenoma, and carcinoma were 42.5%, 71.4%, and 82.3%, respectively, indicating a decreased degree of KLF4 expression over the course of progressive transformation of normal cells into malignant derivatives. KLF4 protein levels showed no correlation with sex, age, or metastatic state (p>0.05), while KLF4 protein expression correlated with the diagnostic stage (p<0.05). Furthermore, strong KLF4 staining was detected in 22.9% (11/48) and 0% (0/14) of well/moderately and poorly differentiated colorectal cancers, respectively. Our results clearly indicate that KLF4 protein expression significantly correlates with the degree of differentiation in colorectal cancers (p<0.05). KLF4 expression in RKO cells is also upregulated by butyrate, an inducer of differentiation.

Conclusions: Downregulation of KLF4 expression may lead to more poorly differentiated tumors.

No MeSH data available.


Related in: MedlinePlus