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Increased number of neurons in the cervical spinal cord of aged female rats.

Portiansky EL, Nishida F, Barbeito CG, Gimeno EJ, Goya RG - PLoS ONE (2011)

Bottom Line: Gross anatomy showed a significant age-related increase in size of all of the cervical segments.Taking the total number of cervical neurons the age-related increase ranged from 19% (C6) to 51% (C3), C3 being the segment that grew most in length in the aged animals.As expected, serum PRL increased markedly with age.

View Article: PubMed Central - PubMed

Affiliation: Laboratorio de Análisis de Imágenes, School of Veterinary Sciences, National University of La Plata, La Plata, Argentina. elporti@fcv.unlp.edu.ar

ABSTRACT
In the brain, specific signaling pathways localized in highly organized regions called niches allow the persistence of a pool of stem and progenitor cells that generate new neurons in adulthood. Much less is known about the spinal cord where a sustained adult neurogenesis is not observed. Moreover, there is scarce information concerning cell proliferation in the adult mammalian spinal cord and virtually none in aging animals or humans. We performed a comparative morphometric and immunofluorescence study of the entire cervical region (C1-C8) in young (5 mo.) and aged (30 mo.) female rats. Serum prolactin (PRL), a neurogenic hormone, was also measured. Gross anatomy showed a significant age-related increase in size of all of the cervical segments. Morphometric analysis of cresyl violet stained segments also showed a significant increase in the area occupied by the gray matter of some cervical segments of aged rats. The most interesting finding was that both the total area occupied by neurons and the number of neurons increased significantly with age, the latter increase ranging from 16% (C6) to 34% (C2). Taking the total number of cervical neurons the age-related increase ranged from 19% (C6) to 51% (C3), C3 being the segment that grew most in length in the aged animals. Some bromodeoxyuridine positive-neuron specific enolase negative (BrdU(+)-NSE(-)) cells were observed and, occasionally, double positive (BrdU(+)-NSE(+)) cells were detected in some cervical segments of both young and aged rats groups. As expected, serum PRL increased markedly with age. We propose that in the cervical spinal cord of female rats, both maturation of pre-existing neuroblasts and/or possible neurogenesis occur during the entire life span, in a process in which PRL may play a role.

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Presence of BrdU positive cells at the cervical spinal cord.A. A C6 young neuron labeled green (NSE) and blue (DAPI) negative for BrdU with a nearby negative glial cell. Bar = 20 µm. B. A NSE[+] – BrdU[+] neuron found in an aged C6 segment. Bar = 10 µm. C. A NSE[−] – BrdU[+] cells, probably corresponding to a glial cell, found in a young C4 segment. Bar = 10 µm.
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pone-0022537-g005: Presence of BrdU positive cells at the cervical spinal cord.A. A C6 young neuron labeled green (NSE) and blue (DAPI) negative for BrdU with a nearby negative glial cell. Bar = 20 µm. B. A NSE[+] – BrdU[+] neuron found in an aged C6 segment. Bar = 10 µm. C. A NSE[−] – BrdU[+] cells, probably corresponding to a glial cell, found in a young C4 segment. Bar = 10 µm.

Mentions: Triple labeling immunofluorescence analysis showed the existence of both NSE[+] - BrdU[+] and NSE[−] - BrdU[+] cells in different segments of animals of both age groups (Fig. 5). In both groups, the frequency of BrdU[+] cells in the cervical segments was very low. This fact made it difficult to statistically detect age-related differences of this parameter.


Increased number of neurons in the cervical spinal cord of aged female rats.

Portiansky EL, Nishida F, Barbeito CG, Gimeno EJ, Goya RG - PLoS ONE (2011)

Presence of BrdU positive cells at the cervical spinal cord.A. A C6 young neuron labeled green (NSE) and blue (DAPI) negative for BrdU with a nearby negative glial cell. Bar = 20 µm. B. A NSE[+] – BrdU[+] neuron found in an aged C6 segment. Bar = 10 µm. C. A NSE[−] – BrdU[+] cells, probably corresponding to a glial cell, found in a young C4 segment. Bar = 10 µm.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3140527&req=5

pone-0022537-g005: Presence of BrdU positive cells at the cervical spinal cord.A. A C6 young neuron labeled green (NSE) and blue (DAPI) negative for BrdU with a nearby negative glial cell. Bar = 20 µm. B. A NSE[+] – BrdU[+] neuron found in an aged C6 segment. Bar = 10 µm. C. A NSE[−] – BrdU[+] cells, probably corresponding to a glial cell, found in a young C4 segment. Bar = 10 µm.
Mentions: Triple labeling immunofluorescence analysis showed the existence of both NSE[+] - BrdU[+] and NSE[−] - BrdU[+] cells in different segments of animals of both age groups (Fig. 5). In both groups, the frequency of BrdU[+] cells in the cervical segments was very low. This fact made it difficult to statistically detect age-related differences of this parameter.

Bottom Line: Gross anatomy showed a significant age-related increase in size of all of the cervical segments.Taking the total number of cervical neurons the age-related increase ranged from 19% (C6) to 51% (C3), C3 being the segment that grew most in length in the aged animals.As expected, serum PRL increased markedly with age.

View Article: PubMed Central - PubMed

Affiliation: Laboratorio de Análisis de Imágenes, School of Veterinary Sciences, National University of La Plata, La Plata, Argentina. elporti@fcv.unlp.edu.ar

ABSTRACT
In the brain, specific signaling pathways localized in highly organized regions called niches allow the persistence of a pool of stem and progenitor cells that generate new neurons in adulthood. Much less is known about the spinal cord where a sustained adult neurogenesis is not observed. Moreover, there is scarce information concerning cell proliferation in the adult mammalian spinal cord and virtually none in aging animals or humans. We performed a comparative morphometric and immunofluorescence study of the entire cervical region (C1-C8) in young (5 mo.) and aged (30 mo.) female rats. Serum prolactin (PRL), a neurogenic hormone, was also measured. Gross anatomy showed a significant age-related increase in size of all of the cervical segments. Morphometric analysis of cresyl violet stained segments also showed a significant increase in the area occupied by the gray matter of some cervical segments of aged rats. The most interesting finding was that both the total area occupied by neurons and the number of neurons increased significantly with age, the latter increase ranging from 16% (C6) to 34% (C2). Taking the total number of cervical neurons the age-related increase ranged from 19% (C6) to 51% (C3), C3 being the segment that grew most in length in the aged animals. Some bromodeoxyuridine positive-neuron specific enolase negative (BrdU(+)-NSE(-)) cells were observed and, occasionally, double positive (BrdU(+)-NSE(+)) cells were detected in some cervical segments of both young and aged rats groups. As expected, serum PRL increased markedly with age. We propose that in the cervical spinal cord of female rats, both maturation of pre-existing neuroblasts and/or possible neurogenesis occur during the entire life span, in a process in which PRL may play a role.

Show MeSH
Related in: MedlinePlus