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Microinjection manipulation resulted in the increased apoptosis of spermatocytes in testes from intracytoplasmic sperm injection (ICSI) derived mice.

Yu Y, Zhao C, Lv Z, Chen W, Tong M, Guo X, Wang L, Liu J, Zhou Z, Zhu H, Zhou Q, Sha J - PLoS ONE (2011)

Bottom Line: However, concerns remain regarding the safety of this method since it is a more invasive procedure than in vitro fertilization (IVF), since a spermatozoon is injected into the oocyte cytoplasm.And, decreased testis weight and marked damage of spermatogenic epithelia were found in aged ICSI mice.The changed expression of a series of proteins relating to apoptosis or proliferation might contribute to it.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Reproductive Biology, Institute of Zoology, the Chinese Academy of Science, Beijing, China.

ABSTRACT
The invention of intracytoplasmic sperm injection (ICSI) has possibly been the most important development in reproductive medicine, one that has given hope to thousands of infertile couples worldwide. However, concerns remain regarding the safety of this method since it is a more invasive procedure than in vitro fertilization (IVF), since a spermatozoon is injected into the oocyte cytoplasm. Using mice derived from IVF technology as a control, we assessed the influence of invasive microinjection in the process of transferring sperm into oocyte cytoplasm in ICSI procedure on the development and physiologic function of resultant offspring. Our results demonstrated that mice produced from ICSI and IVF had no significant difference in phenotypic indices including body weight, forelimb physiology, and learning and memory ability. However, increased spermatocyte apoptosis was observed in the testis of adult ICSI mice, when compared with IVF mice. And, decreased testis weight and marked damage of spermatogenic epithelia were found in aged ICSI mice. Furthermore, proteomic analysis verified that most of the differentiated proteins in testes between adult ICSI and IVF mice were those involved in regulation of apoptosis pathways. Our results demonstrated that the microinjection manipulation used in the ICSI procedure might pose potential risks to the fertility of male offspring. The changed expression of a series of proteins relating to apoptosis or proliferation might contribute to it. Further studies are necessary to better understand all the risks of ICSI.

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Body weight and organ weight.(A) Weight monitoring of mice (4–80 weeks old) showed no significant differences between the ICSI and control mice (p>0.05). (B) Examination of organ weight showed no significant difference in the weight of heart, liver, spleen, lung, kidney and brain between the ICSI and IVF groups, but the testis relative weight of ICSI mice was lower than that of IVF mice (* P<0.05).
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pone-0022172-g002: Body weight and organ weight.(A) Weight monitoring of mice (4–80 weeks old) showed no significant differences between the ICSI and control mice (p>0.05). (B) Examination of organ weight showed no significant difference in the weight of heart, liver, spleen, lung, kidney and brain between the ICSI and IVF groups, but the testis relative weight of ICSI mice was lower than that of IVF mice (* P<0.05).

Mentions: The body weight of ISCI (female n = 7, male n = 6) and IVF (female n = 16, male n = 4) mice at 4∼80 weeks old was examined, and no remarkable differences were detected between these two groups (P>0.05, Fig. 2A). When mice were sacrificed at 86-weeks old, the important organs including heart, liver, spleen, lung, kidney, brain and testis were collected and their relative weight (percentage of organ weight relative to body weight) were analyzed. We found that there were no significant differences in organ weight between the ICSI (n = 11) and IVF (n = 18) groups, except that the testis relative weight of ICSI mice was lower than that of IVF mice (P<0.05; Fig. 2B).


Microinjection manipulation resulted in the increased apoptosis of spermatocytes in testes from intracytoplasmic sperm injection (ICSI) derived mice.

Yu Y, Zhao C, Lv Z, Chen W, Tong M, Guo X, Wang L, Liu J, Zhou Z, Zhu H, Zhou Q, Sha J - PLoS ONE (2011)

Body weight and organ weight.(A) Weight monitoring of mice (4–80 weeks old) showed no significant differences between the ICSI and control mice (p>0.05). (B) Examination of organ weight showed no significant difference in the weight of heart, liver, spleen, lung, kidney and brain between the ICSI and IVF groups, but the testis relative weight of ICSI mice was lower than that of IVF mice (* P<0.05).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3140508&req=5

pone-0022172-g002: Body weight and organ weight.(A) Weight monitoring of mice (4–80 weeks old) showed no significant differences between the ICSI and control mice (p>0.05). (B) Examination of organ weight showed no significant difference in the weight of heart, liver, spleen, lung, kidney and brain between the ICSI and IVF groups, but the testis relative weight of ICSI mice was lower than that of IVF mice (* P<0.05).
Mentions: The body weight of ISCI (female n = 7, male n = 6) and IVF (female n = 16, male n = 4) mice at 4∼80 weeks old was examined, and no remarkable differences were detected between these two groups (P>0.05, Fig. 2A). When mice were sacrificed at 86-weeks old, the important organs including heart, liver, spleen, lung, kidney, brain and testis were collected and their relative weight (percentage of organ weight relative to body weight) were analyzed. We found that there were no significant differences in organ weight between the ICSI (n = 11) and IVF (n = 18) groups, except that the testis relative weight of ICSI mice was lower than that of IVF mice (P<0.05; Fig. 2B).

Bottom Line: However, concerns remain regarding the safety of this method since it is a more invasive procedure than in vitro fertilization (IVF), since a spermatozoon is injected into the oocyte cytoplasm.And, decreased testis weight and marked damage of spermatogenic epithelia were found in aged ICSI mice.The changed expression of a series of proteins relating to apoptosis or proliferation might contribute to it.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Reproductive Biology, Institute of Zoology, the Chinese Academy of Science, Beijing, China.

ABSTRACT
The invention of intracytoplasmic sperm injection (ICSI) has possibly been the most important development in reproductive medicine, one that has given hope to thousands of infertile couples worldwide. However, concerns remain regarding the safety of this method since it is a more invasive procedure than in vitro fertilization (IVF), since a spermatozoon is injected into the oocyte cytoplasm. Using mice derived from IVF technology as a control, we assessed the influence of invasive microinjection in the process of transferring sperm into oocyte cytoplasm in ICSI procedure on the development and physiologic function of resultant offspring. Our results demonstrated that mice produced from ICSI and IVF had no significant difference in phenotypic indices including body weight, forelimb physiology, and learning and memory ability. However, increased spermatocyte apoptosis was observed in the testis of adult ICSI mice, when compared with IVF mice. And, decreased testis weight and marked damage of spermatogenic epithelia were found in aged ICSI mice. Furthermore, proteomic analysis verified that most of the differentiated proteins in testes between adult ICSI and IVF mice were those involved in regulation of apoptosis pathways. Our results demonstrated that the microinjection manipulation used in the ICSI procedure might pose potential risks to the fertility of male offspring. The changed expression of a series of proteins relating to apoptosis or proliferation might contribute to it. Further studies are necessary to better understand all the risks of ICSI.

Show MeSH
Related in: MedlinePlus