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Microinjection manipulation resulted in the increased apoptosis of spermatocytes in testes from intracytoplasmic sperm injection (ICSI) derived mice.

Yu Y, Zhao C, Lv Z, Chen W, Tong M, Guo X, Wang L, Liu J, Zhou Z, Zhu H, Zhou Q, Sha J - PLoS ONE (2011)

Bottom Line: However, concerns remain regarding the safety of this method since it is a more invasive procedure than in vitro fertilization (IVF), since a spermatozoon is injected into the oocyte cytoplasm.And, decreased testis weight and marked damage of spermatogenic epithelia were found in aged ICSI mice.The changed expression of a series of proteins relating to apoptosis or proliferation might contribute to it.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Reproductive Biology, Institute of Zoology, the Chinese Academy of Science, Beijing, China.

ABSTRACT
The invention of intracytoplasmic sperm injection (ICSI) has possibly been the most important development in reproductive medicine, one that has given hope to thousands of infertile couples worldwide. However, concerns remain regarding the safety of this method since it is a more invasive procedure than in vitro fertilization (IVF), since a spermatozoon is injected into the oocyte cytoplasm. Using mice derived from IVF technology as a control, we assessed the influence of invasive microinjection in the process of transferring sperm into oocyte cytoplasm in ICSI procedure on the development and physiologic function of resultant offspring. Our results demonstrated that mice produced from ICSI and IVF had no significant difference in phenotypic indices including body weight, forelimb physiology, and learning and memory ability. However, increased spermatocyte apoptosis was observed in the testis of adult ICSI mice, when compared with IVF mice. And, decreased testis weight and marked damage of spermatogenic epithelia were found in aged ICSI mice. Furthermore, proteomic analysis verified that most of the differentiated proteins in testes between adult ICSI and IVF mice were those involved in regulation of apoptosis pathways. Our results demonstrated that the microinjection manipulation used in the ICSI procedure might pose potential risks to the fertility of male offspring. The changed expression of a series of proteins relating to apoptosis or proliferation might contribute to it. Further studies are necessary to better understand all the risks of ICSI.

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Results from the Morris water task showed there was no significant difference in acquisition trials, probe tests and reversal trials between ICSI and IVF groups (P>0.05).(A) Average latency to find hidden platform - acquisition trials (Adult mice). (B) Average time spent searching quadrant in which animal trained vs. other quadrants - probe tests (Adult mice). (C) Average latency to find hidden platform - reversal trials (Adult mice). (D) Average latency to find hidden platform - acquisition trials (Aged mice).
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pone-0022172-g001: Results from the Morris water task showed there was no significant difference in acquisition trials, probe tests and reversal trials between ICSI and IVF groups (P>0.05).(A) Average latency to find hidden platform - acquisition trials (Adult mice). (B) Average time spent searching quadrant in which animal trained vs. other quadrants - probe tests (Adult mice). (C) Average latency to find hidden platform - reversal trials (Adult mice). (D) Average latency to find hidden platform - acquisition trials (Aged mice).

Mentions: To study the spatial learning and memory ability of adult mice conceived by ICSI and IVF, a total of 36 trials were performed during 6 consecutive days by using a Morris water maze apparatus, on 10 mice (5/group). In acquisition trials, the results showed that both ICSI and IVF mice displayed evident improvement over the 3 days of testing, and the mean acquisition times in the two groups did not differ significantly from each other (P>0.05, Fig. 1A). In the probe tests, we found that the time that mice wandered in the fourth quadrant was longer than that in the other three quadrants, but there was no difference between the ICSI and IVF groups (P>0.05, Fig. 1B). In the reverse trials, the hidden platform was relocated into the opposite second quadrant, and the mice were retested. Both groups improved their performance over the 2 days of trials, with no significant difference observed between the two groups (P>0.05, Fig. 1C). Furthermore, to test whether the nervous function was influenced by ICSI manipulation with age, the Morris water maze was also performed in 24 (12/group)aged mice. In acquisition trials, the mean acquisition times also had no significant difference between the two groups (P>0.05, Fig. 1D). However, the aged mice failed to complete the probe trials and the reversal trials due to their poor health. Thus, the memory function could not be evaluated in the aged offspring.


Microinjection manipulation resulted in the increased apoptosis of spermatocytes in testes from intracytoplasmic sperm injection (ICSI) derived mice.

Yu Y, Zhao C, Lv Z, Chen W, Tong M, Guo X, Wang L, Liu J, Zhou Z, Zhu H, Zhou Q, Sha J - PLoS ONE (2011)

Results from the Morris water task showed there was no significant difference in acquisition trials, probe tests and reversal trials between ICSI and IVF groups (P>0.05).(A) Average latency to find hidden platform - acquisition trials (Adult mice). (B) Average time spent searching quadrant in which animal trained vs. other quadrants - probe tests (Adult mice). (C) Average latency to find hidden platform - reversal trials (Adult mice). (D) Average latency to find hidden platform - acquisition trials (Aged mice).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3140508&req=5

pone-0022172-g001: Results from the Morris water task showed there was no significant difference in acquisition trials, probe tests and reversal trials between ICSI and IVF groups (P>0.05).(A) Average latency to find hidden platform - acquisition trials (Adult mice). (B) Average time spent searching quadrant in which animal trained vs. other quadrants - probe tests (Adult mice). (C) Average latency to find hidden platform - reversal trials (Adult mice). (D) Average latency to find hidden platform - acquisition trials (Aged mice).
Mentions: To study the spatial learning and memory ability of adult mice conceived by ICSI and IVF, a total of 36 trials were performed during 6 consecutive days by using a Morris water maze apparatus, on 10 mice (5/group). In acquisition trials, the results showed that both ICSI and IVF mice displayed evident improvement over the 3 days of testing, and the mean acquisition times in the two groups did not differ significantly from each other (P>0.05, Fig. 1A). In the probe tests, we found that the time that mice wandered in the fourth quadrant was longer than that in the other three quadrants, but there was no difference between the ICSI and IVF groups (P>0.05, Fig. 1B). In the reverse trials, the hidden platform was relocated into the opposite second quadrant, and the mice were retested. Both groups improved their performance over the 2 days of trials, with no significant difference observed between the two groups (P>0.05, Fig. 1C). Furthermore, to test whether the nervous function was influenced by ICSI manipulation with age, the Morris water maze was also performed in 24 (12/group)aged mice. In acquisition trials, the mean acquisition times also had no significant difference between the two groups (P>0.05, Fig. 1D). However, the aged mice failed to complete the probe trials and the reversal trials due to their poor health. Thus, the memory function could not be evaluated in the aged offspring.

Bottom Line: However, concerns remain regarding the safety of this method since it is a more invasive procedure than in vitro fertilization (IVF), since a spermatozoon is injected into the oocyte cytoplasm.And, decreased testis weight and marked damage of spermatogenic epithelia were found in aged ICSI mice.The changed expression of a series of proteins relating to apoptosis or proliferation might contribute to it.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Reproductive Biology, Institute of Zoology, the Chinese Academy of Science, Beijing, China.

ABSTRACT
The invention of intracytoplasmic sperm injection (ICSI) has possibly been the most important development in reproductive medicine, one that has given hope to thousands of infertile couples worldwide. However, concerns remain regarding the safety of this method since it is a more invasive procedure than in vitro fertilization (IVF), since a spermatozoon is injected into the oocyte cytoplasm. Using mice derived from IVF technology as a control, we assessed the influence of invasive microinjection in the process of transferring sperm into oocyte cytoplasm in ICSI procedure on the development and physiologic function of resultant offspring. Our results demonstrated that mice produced from ICSI and IVF had no significant difference in phenotypic indices including body weight, forelimb physiology, and learning and memory ability. However, increased spermatocyte apoptosis was observed in the testis of adult ICSI mice, when compared with IVF mice. And, decreased testis weight and marked damage of spermatogenic epithelia were found in aged ICSI mice. Furthermore, proteomic analysis verified that most of the differentiated proteins in testes between adult ICSI and IVF mice were those involved in regulation of apoptosis pathways. Our results demonstrated that the microinjection manipulation used in the ICSI procedure might pose potential risks to the fertility of male offspring. The changed expression of a series of proteins relating to apoptosis or proliferation might contribute to it. Further studies are necessary to better understand all the risks of ICSI.

Show MeSH
Related in: MedlinePlus