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Conductimetric biosensor for the detection of uric Acid by immobilization uricase on nata de coco membrane-pt electrode.

Mulyasuryani A, Srihardiastutie A - Anal Chem Insights (2011)

Bottom Line: The response is affected by the membrane thickness and pH change in the range 7.5-9.5.The response time is three minutes in aqueous solutions and in human serum samples.The operational stability of the biosensor was not less than three days and the relative error is smaller than 10%.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemistry, Brawijaya University, Jl. Veteran, Malang, 65145, Indonesia.

ABSTRACT
A conductimetric enzyme biosensor for uric acid detection has been developed. The uricase, as enzyme, is isolated from Candida utilis and immobilized on a nata de coco membrane-Pt electrode. The biosensor demonstrates a linear response to urate over the concentration range 1-6 ppm and has good selectivity properties. The response is affected by the membrane thickness and pH change in the range 7.5-9.5. The response time is three minutes in aqueous solutions and in human serum samples. Application of the biosensor to the determination of uric acid in human serum gave results that compared favourably with those obtained by medical laboratory. The operational stability of the biosensor was not less than three days and the relative error is smaller than 10%.

No MeSH data available.


Determination of the concentration range of uric acid.
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Related In: Results  -  Collection


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f5-aci-6-2011-047: Determination of the concentration range of uric acid.

Mentions: Figure 5 is a curve to determine the concentration range of uric acid. The curve shows two linear equations and the intercept shows the maximum concentration of uric acid which can be determined by the biosensor. The concentration range of uric acid is 1–6 ppm.


Conductimetric biosensor for the detection of uric Acid by immobilization uricase on nata de coco membrane-pt electrode.

Mulyasuryani A, Srihardiastutie A - Anal Chem Insights (2011)

Determination of the concentration range of uric acid.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3140265&req=5

f5-aci-6-2011-047: Determination of the concentration range of uric acid.
Mentions: Figure 5 is a curve to determine the concentration range of uric acid. The curve shows two linear equations and the intercept shows the maximum concentration of uric acid which can be determined by the biosensor. The concentration range of uric acid is 1–6 ppm.

Bottom Line: The response is affected by the membrane thickness and pH change in the range 7.5-9.5.The response time is three minutes in aqueous solutions and in human serum samples.The operational stability of the biosensor was not less than three days and the relative error is smaller than 10%.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemistry, Brawijaya University, Jl. Veteran, Malang, 65145, Indonesia.

ABSTRACT
A conductimetric enzyme biosensor for uric acid detection has been developed. The uricase, as enzyme, is isolated from Candida utilis and immobilized on a nata de coco membrane-Pt electrode. The biosensor demonstrates a linear response to urate over the concentration range 1-6 ppm and has good selectivity properties. The response is affected by the membrane thickness and pH change in the range 7.5-9.5. The response time is three minutes in aqueous solutions and in human serum samples. Application of the biosensor to the determination of uric acid in human serum gave results that compared favourably with those obtained by medical laboratory. The operational stability of the biosensor was not less than three days and the relative error is smaller than 10%.

No MeSH data available.