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Conductimetric biosensor for the detection of uric Acid by immobilization uricase on nata de coco membrane-pt electrode.

Mulyasuryani A, Srihardiastutie A - Anal Chem Insights (2011)

Bottom Line: The response is affected by the membrane thickness and pH change in the range 7.5-9.5.The response time is three minutes in aqueous solutions and in human serum samples.The operational stability of the biosensor was not less than three days and the relative error is smaller than 10%.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemistry, Brawijaya University, Jl. Veteran, Malang, 65145, Indonesia.

ABSTRACT
A conductimetric enzyme biosensor for uric acid detection has been developed. The uricase, as enzyme, is isolated from Candida utilis and immobilized on a nata de coco membrane-Pt electrode. The biosensor demonstrates a linear response to urate over the concentration range 1-6 ppm and has good selectivity properties. The response is affected by the membrane thickness and pH change in the range 7.5-9.5. The response time is three minutes in aqueous solutions and in human serum samples. Application of the biosensor to the determination of uric acid in human serum gave results that compared favourably with those obtained by medical laboratory. The operational stability of the biosensor was not less than three days and the relative error is smaller than 10%.

No MeSH data available.


The relation of measuring time and conductivity at 1–6 ppm uric acid concentrations.
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f4-aci-6-2011-047: The relation of measuring time and conductivity at 1–6 ppm uric acid concentrations.

Mentions: Response time is the time to allow the system to come to equilibrium, which is indicated by the signal stability. 13 Figure 4 illustrates the relation of measuring time and conductivity at several concentrations of uric acid. The conductivity decreases consistently for all concentrations. This phenomenon is caused by the amount of the uric acid loading on the membrane pores. Initially, the ions to arrive to the electrode surface are ions from the buffer solution (Na+, K+, OH−, HPO4 =), so the conductivity is caused by these ions. The conductivity of the blank solution (phosphate buffer) averages at 250 μS, which is higher than the conductivity of the uric acid solution. The response time was determined from Figure 4, which is the starting time where the signal is constant; the response time for all concentrations is 3 minutes.


Conductimetric biosensor for the detection of uric Acid by immobilization uricase on nata de coco membrane-pt electrode.

Mulyasuryani A, Srihardiastutie A - Anal Chem Insights (2011)

The relation of measuring time and conductivity at 1–6 ppm uric acid concentrations.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3140265&req=5

f4-aci-6-2011-047: The relation of measuring time and conductivity at 1–6 ppm uric acid concentrations.
Mentions: Response time is the time to allow the system to come to equilibrium, which is indicated by the signal stability. 13 Figure 4 illustrates the relation of measuring time and conductivity at several concentrations of uric acid. The conductivity decreases consistently for all concentrations. This phenomenon is caused by the amount of the uric acid loading on the membrane pores. Initially, the ions to arrive to the electrode surface are ions from the buffer solution (Na+, K+, OH−, HPO4 =), so the conductivity is caused by these ions. The conductivity of the blank solution (phosphate buffer) averages at 250 μS, which is higher than the conductivity of the uric acid solution. The response time was determined from Figure 4, which is the starting time where the signal is constant; the response time for all concentrations is 3 minutes.

Bottom Line: The response is affected by the membrane thickness and pH change in the range 7.5-9.5.The response time is three minutes in aqueous solutions and in human serum samples.The operational stability of the biosensor was not less than three days and the relative error is smaller than 10%.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemistry, Brawijaya University, Jl. Veteran, Malang, 65145, Indonesia.

ABSTRACT
A conductimetric enzyme biosensor for uric acid detection has been developed. The uricase, as enzyme, is isolated from Candida utilis and immobilized on a nata de coco membrane-Pt electrode. The biosensor demonstrates a linear response to urate over the concentration range 1-6 ppm and has good selectivity properties. The response is affected by the membrane thickness and pH change in the range 7.5-9.5. The response time is three minutes in aqueous solutions and in human serum samples. Application of the biosensor to the determination of uric acid in human serum gave results that compared favourably with those obtained by medical laboratory. The operational stability of the biosensor was not less than three days and the relative error is smaller than 10%.

No MeSH data available.