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Conductimetric biosensor for the detection of uric Acid by immobilization uricase on nata de coco membrane-pt electrode.

Mulyasuryani A, Srihardiastutie A - Anal Chem Insights (2011)

Bottom Line: The response is affected by the membrane thickness and pH change in the range 7.5-9.5.The response time is three minutes in aqueous solutions and in human serum samples.The operational stability of the biosensor was not less than three days and the relative error is smaller than 10%.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemistry, Brawijaya University, Jl. Veteran, Malang, 65145, Indonesia.

ABSTRACT
A conductimetric enzyme biosensor for uric acid detection has been developed. The uricase, as enzyme, is isolated from Candida utilis and immobilized on a nata de coco membrane-Pt electrode. The biosensor demonstrates a linear response to urate over the concentration range 1-6 ppm and has good selectivity properties. The response is affected by the membrane thickness and pH change in the range 7.5-9.5. The response time is three minutes in aqueous solutions and in human serum samples. Application of the biosensor to the determination of uric acid in human serum gave results that compared favourably with those obtained by medical laboratory. The operational stability of the biosensor was not less than three days and the relative error is smaller than 10%.

No MeSH data available.


Curve of relation uric acid concentration to conductivity in various solution of pH.
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f3-aci-6-2011-047: Curve of relation uric acid concentration to conductivity in various solution of pH.

Mentions: In this research, the pH range used is 7.5 up to 9.5. There are two reasons. First, in previous works, the maximum immobilized uricase activities are at pH 8–9. Second, the number of HCO3− ions as an oxidation product of uric acid is present (>90%) at pH 7.5–9.5. Figure 3 illustrates that the conductivity is not significantly different for all concentrations of uric acid (1–10 ppm) at pH 8–9.5. At pH 7.5, conductivities show significant dynamic linear correlations with the uric acid concentrations at 1–3 ppm.


Conductimetric biosensor for the detection of uric Acid by immobilization uricase on nata de coco membrane-pt electrode.

Mulyasuryani A, Srihardiastutie A - Anal Chem Insights (2011)

Curve of relation uric acid concentration to conductivity in various solution of pH.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3140265&req=5

f3-aci-6-2011-047: Curve of relation uric acid concentration to conductivity in various solution of pH.
Mentions: In this research, the pH range used is 7.5 up to 9.5. There are two reasons. First, in previous works, the maximum immobilized uricase activities are at pH 8–9. Second, the number of HCO3− ions as an oxidation product of uric acid is present (>90%) at pH 7.5–9.5. Figure 3 illustrates that the conductivity is not significantly different for all concentrations of uric acid (1–10 ppm) at pH 8–9.5. At pH 7.5, conductivities show significant dynamic linear correlations with the uric acid concentrations at 1–3 ppm.

Bottom Line: The response is affected by the membrane thickness and pH change in the range 7.5-9.5.The response time is three minutes in aqueous solutions and in human serum samples.The operational stability of the biosensor was not less than three days and the relative error is smaller than 10%.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemistry, Brawijaya University, Jl. Veteran, Malang, 65145, Indonesia.

ABSTRACT
A conductimetric enzyme biosensor for uric acid detection has been developed. The uricase, as enzyme, is isolated from Candida utilis and immobilized on a nata de coco membrane-Pt electrode. The biosensor demonstrates a linear response to urate over the concentration range 1-6 ppm and has good selectivity properties. The response is affected by the membrane thickness and pH change in the range 7.5-9.5. The response time is three minutes in aqueous solutions and in human serum samples. Application of the biosensor to the determination of uric acid in human serum gave results that compared favourably with those obtained by medical laboratory. The operational stability of the biosensor was not less than three days and the relative error is smaller than 10%.

No MeSH data available.