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Amelioratory Effect of Nanoconjugated Vancomycin on Spleen during VRSA-Induced Oxidative Stress.

Chakraborty SP, Karmahapatra S, Sahu SK, Pramanik P, Roy S - Patholog Res Int (2011)

Bottom Line: VRSA-infected mice were treated with nanoconjugated vancomycin at its effective dose for 10 days.Conclusion.These findings suggest the potential use and beneficial role of nanoconjugated vancomycin against VRSA-infection-induced oxidative stress and DNA damage in spleen.

View Article: PubMed Central - PubMed

Affiliation: Immunology and Microbiology Laboratory, Department of Human Physiology with Community Health, Vidyasagar University, West Bengal, Midnapore 721 102, India.

ABSTRACT
Objective. The aim of the present study was to evaluate the possible antioxidant effects of nanoconjugated vancomycin against VRSA infection on select makers of oxidative damage and antioxidant status in spleen. Methods. A coagulase-positive VRSA strain was used for this study. VRSA infection was developed in Swiss mice by intraperitoneal injection of 5 × 10(6) CFU/mL bacterial solutions. VRSA-infected mice were treated with nanoconjugated vancomycin at its effective dose for 10 days. After decapitation, blood was used for determination of viable bacteria count and spleen was excised from control and experimental groups, homogenized and used for different biochemical estimations. Results. Nitrate level, myeloperoxidase activity, lipid peroxidation, protein oxidation, oxidized glutathione, and DNA fragmentation level were increased significantly (P < 0.05) in spleen of VRSA-infected group as compared to control group, and reduced glutathione level, activity of SOD, CAT, GPx, GR, and GST were decreased significantly (P < 0.05); which were increased or decreased significantly (P < 0.05) near to normal in nanoconjugated vancomycin-treated group. Conclusion. These findings suggest the potential use and beneficial role of nanoconjugated vancomycin against VRSA-infection-induced oxidative stress and DNA damage in spleen.

No MeSH data available.


Related in: MedlinePlus

Superoxide dismutase (SOD) and catalase (CAT) activity in spleen of control, VRSA-infected, and nanoconjugated-vancomycin-treated group. Values are expressed as mean ± SEM, n = 6. *indicates significant difference (P < 0.05) compared to control group.  #indicates significant difference (P < 0.05) compared to VRSA-infected group.
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fig6: Superoxide dismutase (SOD) and catalase (CAT) activity in spleen of control, VRSA-infected, and nanoconjugated-vancomycin-treated group. Values are expressed as mean ± SEM, n = 6. *indicates significant difference (P < 0.05) compared to control group. #indicates significant difference (P < 0.05) compared to VRSA-infected group.

Mentions: The super oxide dismutase (SOD) and catalase (CAT) activities were measured to understand the antioxidant enzymes status in spleen of VRSA-infected group. SOD and CAT activities were decreased significantly (P < 0.05) by 63.31% and 56.95% in spleen of VRSA-infected group, respectively, as compared to control, in which they were significantly (P < 0.05) increased by 133.78% and 95.62% due to treatment of nanoconjugated vancomycin (Figure 6).


Amelioratory Effect of Nanoconjugated Vancomycin on Spleen during VRSA-Induced Oxidative Stress.

Chakraborty SP, Karmahapatra S, Sahu SK, Pramanik P, Roy S - Patholog Res Int (2011)

Superoxide dismutase (SOD) and catalase (CAT) activity in spleen of control, VRSA-infected, and nanoconjugated-vancomycin-treated group. Values are expressed as mean ± SEM, n = 6. *indicates significant difference (P < 0.05) compared to control group.  #indicates significant difference (P < 0.05) compared to VRSA-infected group.
© Copyright Policy - open-access
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC3140227&req=5

fig6: Superoxide dismutase (SOD) and catalase (CAT) activity in spleen of control, VRSA-infected, and nanoconjugated-vancomycin-treated group. Values are expressed as mean ± SEM, n = 6. *indicates significant difference (P < 0.05) compared to control group. #indicates significant difference (P < 0.05) compared to VRSA-infected group.
Mentions: The super oxide dismutase (SOD) and catalase (CAT) activities were measured to understand the antioxidant enzymes status in spleen of VRSA-infected group. SOD and CAT activities were decreased significantly (P < 0.05) by 63.31% and 56.95% in spleen of VRSA-infected group, respectively, as compared to control, in which they were significantly (P < 0.05) increased by 133.78% and 95.62% due to treatment of nanoconjugated vancomycin (Figure 6).

Bottom Line: VRSA-infected mice were treated with nanoconjugated vancomycin at its effective dose for 10 days.Conclusion.These findings suggest the potential use and beneficial role of nanoconjugated vancomycin against VRSA-infection-induced oxidative stress and DNA damage in spleen.

View Article: PubMed Central - PubMed

Affiliation: Immunology and Microbiology Laboratory, Department of Human Physiology with Community Health, Vidyasagar University, West Bengal, Midnapore 721 102, India.

ABSTRACT
Objective. The aim of the present study was to evaluate the possible antioxidant effects of nanoconjugated vancomycin against VRSA infection on select makers of oxidative damage and antioxidant status in spleen. Methods. A coagulase-positive VRSA strain was used for this study. VRSA infection was developed in Swiss mice by intraperitoneal injection of 5 × 10(6) CFU/mL bacterial solutions. VRSA-infected mice were treated with nanoconjugated vancomycin at its effective dose for 10 days. After decapitation, blood was used for determination of viable bacteria count and spleen was excised from control and experimental groups, homogenized and used for different biochemical estimations. Results. Nitrate level, myeloperoxidase activity, lipid peroxidation, protein oxidation, oxidized glutathione, and DNA fragmentation level were increased significantly (P < 0.05) in spleen of VRSA-infected group as compared to control group, and reduced glutathione level, activity of SOD, CAT, GPx, GR, and GST were decreased significantly (P < 0.05); which were increased or decreased significantly (P < 0.05) near to normal in nanoconjugated vancomycin-treated group. Conclusion. These findings suggest the potential use and beneficial role of nanoconjugated vancomycin against VRSA-infection-induced oxidative stress and DNA damage in spleen.

No MeSH data available.


Related in: MedlinePlus