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Amelioratory Effect of Nanoconjugated Vancomycin on Spleen during VRSA-Induced Oxidative Stress.

Chakraborty SP, Karmahapatra S, Sahu SK, Pramanik P, Roy S - Patholog Res Int (2011)

Bottom Line: VRSA-infected mice were treated with nanoconjugated vancomycin at its effective dose for 10 days.Conclusion.These findings suggest the potential use and beneficial role of nanoconjugated vancomycin against VRSA-infection-induced oxidative stress and DNA damage in spleen.

View Article: PubMed Central - PubMed

Affiliation: Immunology and Microbiology Laboratory, Department of Human Physiology with Community Health, Vidyasagar University, West Bengal, Midnapore 721 102, India.

ABSTRACT
Objective. The aim of the present study was to evaluate the possible antioxidant effects of nanoconjugated vancomycin against VRSA infection on select makers of oxidative damage and antioxidant status in spleen. Methods. A coagulase-positive VRSA strain was used for this study. VRSA infection was developed in Swiss mice by intraperitoneal injection of 5 × 10(6) CFU/mL bacterial solutions. VRSA-infected mice were treated with nanoconjugated vancomycin at its effective dose for 10 days. After decapitation, blood was used for determination of viable bacteria count and spleen was excised from control and experimental groups, homogenized and used for different biochemical estimations. Results. Nitrate level, myeloperoxidase activity, lipid peroxidation, protein oxidation, oxidized glutathione, and DNA fragmentation level were increased significantly (P < 0.05) in spleen of VRSA-infected group as compared to control group, and reduced glutathione level, activity of SOD, CAT, GPx, GR, and GST were decreased significantly (P < 0.05); which were increased or decreased significantly (P < 0.05) near to normal in nanoconjugated vancomycin-treated group. Conclusion. These findings suggest the potential use and beneficial role of nanoconjugated vancomycin against VRSA-infection-induced oxidative stress and DNA damage in spleen.

No MeSH data available.


Related in: MedlinePlus

Photomicrographs of the histopathological analysis of the mice spleen tissues (400x). (a) Control group: normal red pulp  (arrow) and white pulp  (black diamond); (b) VRSA-infected group: degeneration of red pulp (arrow) and white pulp (black diamond); (c) nanoconjugated-vancomycin-treated group: red pulp  (arrow) and white pulp (black diamond).
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fig1: Photomicrographs of the histopathological analysis of the mice spleen tissues (400x). (a) Control group: normal red pulp (arrow) and white pulp (black diamond); (b) VRSA-infected group: degeneration of red pulp (arrow) and white pulp (black diamond); (c) nanoconjugated-vancomycin-treated group: red pulp (arrow) and white pulp (black diamond).

Mentions: Histopathological analysis revealed that VRSA infection resulted in severe pathological changes and tissue injury in spleen. On the other hand, treatment of nanoconjugated vancomycin showed recovery of tissue damage in spleen. This was confirmed by histopathological examinations of the spleen. Microscopic observations of control spleen showed regular structure of red pulp and white pulp (Figure 1(a)) while in VRSA-infected group the spleen showed degeneration of red pulp and white pulp (Figure 1(b)). Treatment of nanoconjugated vancomycin in VRSA-infection group caused regeneration of red pulp (Figure 1(c)).


Amelioratory Effect of Nanoconjugated Vancomycin on Spleen during VRSA-Induced Oxidative Stress.

Chakraborty SP, Karmahapatra S, Sahu SK, Pramanik P, Roy S - Patholog Res Int (2011)

Photomicrographs of the histopathological analysis of the mice spleen tissues (400x). (a) Control group: normal red pulp  (arrow) and white pulp  (black diamond); (b) VRSA-infected group: degeneration of red pulp (arrow) and white pulp (black diamond); (c) nanoconjugated-vancomycin-treated group: red pulp  (arrow) and white pulp (black diamond).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3140227&req=5

fig1: Photomicrographs of the histopathological analysis of the mice spleen tissues (400x). (a) Control group: normal red pulp (arrow) and white pulp (black diamond); (b) VRSA-infected group: degeneration of red pulp (arrow) and white pulp (black diamond); (c) nanoconjugated-vancomycin-treated group: red pulp (arrow) and white pulp (black diamond).
Mentions: Histopathological analysis revealed that VRSA infection resulted in severe pathological changes and tissue injury in spleen. On the other hand, treatment of nanoconjugated vancomycin showed recovery of tissue damage in spleen. This was confirmed by histopathological examinations of the spleen. Microscopic observations of control spleen showed regular structure of red pulp and white pulp (Figure 1(a)) while in VRSA-infected group the spleen showed degeneration of red pulp and white pulp (Figure 1(b)). Treatment of nanoconjugated vancomycin in VRSA-infection group caused regeneration of red pulp (Figure 1(c)).

Bottom Line: VRSA-infected mice were treated with nanoconjugated vancomycin at its effective dose for 10 days.Conclusion.These findings suggest the potential use and beneficial role of nanoconjugated vancomycin against VRSA-infection-induced oxidative stress and DNA damage in spleen.

View Article: PubMed Central - PubMed

Affiliation: Immunology and Microbiology Laboratory, Department of Human Physiology with Community Health, Vidyasagar University, West Bengal, Midnapore 721 102, India.

ABSTRACT
Objective. The aim of the present study was to evaluate the possible antioxidant effects of nanoconjugated vancomycin against VRSA infection on select makers of oxidative damage and antioxidant status in spleen. Methods. A coagulase-positive VRSA strain was used for this study. VRSA infection was developed in Swiss mice by intraperitoneal injection of 5 × 10(6) CFU/mL bacterial solutions. VRSA-infected mice were treated with nanoconjugated vancomycin at its effective dose for 10 days. After decapitation, blood was used for determination of viable bacteria count and spleen was excised from control and experimental groups, homogenized and used for different biochemical estimations. Results. Nitrate level, myeloperoxidase activity, lipid peroxidation, protein oxidation, oxidized glutathione, and DNA fragmentation level were increased significantly (P < 0.05) in spleen of VRSA-infected group as compared to control group, and reduced glutathione level, activity of SOD, CAT, GPx, GR, and GST were decreased significantly (P < 0.05); which were increased or decreased significantly (P < 0.05) near to normal in nanoconjugated vancomycin-treated group. Conclusion. These findings suggest the potential use and beneficial role of nanoconjugated vancomycin against VRSA-infection-induced oxidative stress and DNA damage in spleen.

No MeSH data available.


Related in: MedlinePlus