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Antidiabetic Activity of Gnidia glauca and Dioscorea bulbifera: Potent Amylase and Glucosidase Inhibitors.

Ghosh S, Ahire M, Patil S, Jabgunde A, Bhat Dusane M, Joshi BN, Pardesi K, Jachak S, Dhavale DD, Chopade BA - Evid Based Complement Alternat Med (2011)

Bottom Line: Two plants with significant traditional therapeutic potential, namely, Gnidia glauca and Dioscorea bulbifera, were tested for their efficiency to inhibit α-amylase and α-glucosidase.Petroleum ether extract of flower of G. glauca was found to inhibit α-amylase significantly (78.56%).These results provide intense rationale for further in vivo and clinical study.

View Article: PubMed Central - PubMed

Affiliation: Institute of Bioinformatics and Biotechnology, University of Pune, Pune 411007, India.

ABSTRACT
Diabetes is a metabolic disorder affecting about 220 million people worldwide. One of the most critical complications of diabetes is post-prandial hyper-glycemia (PPHG). Glucosidase inhibitor and α-amylase inhibitors are class of compounds that help in managing PPHG. Low-cost herbal treatment is recommended due to their lesser side effect for treatment of diabetes. Two plants with significant traditional therapeutic potential, namely, Gnidia glauca and Dioscorea bulbifera, were tested for their efficiency to inhibit α-amylase and α-glucosidase. Stem, leaf, and flower of G. glauca and bulb of D. bulbifera were sequentially extracted with petroleum ether, ethyl acetate, and methanol as well as separately with 70% ethanol. Petroleum ether extract of flower of G. glauca was found to inhibit α-amylase significantly (78.56%). Extracts were further tested against crude murine pancreatic, small intestinal, and liver glucosidase enzyme which revealed excellent inhibitory properties. α-glucosidase inhibition provided a strong in vitro evidence for confirmation of both G. glauca and D. bulbifera as excellent antidiabetic remedy. This is the first report of its kind that provides a strong biochemical basis for management of type II diabetes using G. glauca and D. bulbifera. These results provide intense rationale for further in vivo and clinical study.

No MeSH data available.


Related in: MedlinePlus

Representative dose response curves for plant extracts. (a) Petroleum ether extracts against porcine pancreatic α-amylase; (b) ethyl acetate extracts against murine pancreatic glucosidase; (c) petroleum ether extracts against murine small intestinal glucosidase; (d) petroleum ether extracts against murine liver glucosidase: (e) ethyl acetate extracts against α-glucosidase.
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fig4: Representative dose response curves for plant extracts. (a) Petroleum ether extracts against porcine pancreatic α-amylase; (b) ethyl acetate extracts against murine pancreatic glucosidase; (c) petroleum ether extracts against murine small intestinal glucosidase; (d) petroleum ether extracts against murine liver glucosidase: (e) ethyl acetate extracts against α-glucosidase.

Mentions: Plant extracts are known to be potent α-amlase inhibitors due to their rich phenolic content that bind to the reactive sites of enzymes, thus altering its catalytic activity [44]. It has been suggested that the mechanism of inhibition of α-amylase may occur through the direct blockage of the active centre at several subsites of the enzyme as also suggested for other inhibitors [45]. A significant variability in the levels of enzyme inhibition between the parts of the same plant evident in the dose response curves (Figure 4) suggests the phytochemical diversity among the parts resulting in variation in the antidiabetic property. Dose response curves for all extracts against, α-amylase, murine pancreatic, intestinal, liver glucosidases, and α-glucosidase are given in the supplementary information. Our study revealed that petroleum ether extract of G. glauca flower (78.56%) and methanolic extract of D. bulbifera bulbs (73.54%) were potent α-amylase inhibitors. Various herbal extracts like Bougainvillea spectabilis and Trigonella foenum-graecum seeds are known as  α-amylase inhibitors showing 29.43% and 59%, respectively [35, 46]. Thus, G. glauca and D. bulbifera exhibited comparatively more efficient α-amylase inhibition.


Antidiabetic Activity of Gnidia glauca and Dioscorea bulbifera: Potent Amylase and Glucosidase Inhibitors.

Ghosh S, Ahire M, Patil S, Jabgunde A, Bhat Dusane M, Joshi BN, Pardesi K, Jachak S, Dhavale DD, Chopade BA - Evid Based Complement Alternat Med (2011)

Representative dose response curves for plant extracts. (a) Petroleum ether extracts against porcine pancreatic α-amylase; (b) ethyl acetate extracts against murine pancreatic glucosidase; (c) petroleum ether extracts against murine small intestinal glucosidase; (d) petroleum ether extracts against murine liver glucosidase: (e) ethyl acetate extracts against α-glucosidase.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3140190&req=5

fig4: Representative dose response curves for plant extracts. (a) Petroleum ether extracts against porcine pancreatic α-amylase; (b) ethyl acetate extracts against murine pancreatic glucosidase; (c) petroleum ether extracts against murine small intestinal glucosidase; (d) petroleum ether extracts against murine liver glucosidase: (e) ethyl acetate extracts against α-glucosidase.
Mentions: Plant extracts are known to be potent α-amlase inhibitors due to their rich phenolic content that bind to the reactive sites of enzymes, thus altering its catalytic activity [44]. It has been suggested that the mechanism of inhibition of α-amylase may occur through the direct blockage of the active centre at several subsites of the enzyme as also suggested for other inhibitors [45]. A significant variability in the levels of enzyme inhibition between the parts of the same plant evident in the dose response curves (Figure 4) suggests the phytochemical diversity among the parts resulting in variation in the antidiabetic property. Dose response curves for all extracts against, α-amylase, murine pancreatic, intestinal, liver glucosidases, and α-glucosidase are given in the supplementary information. Our study revealed that petroleum ether extract of G. glauca flower (78.56%) and methanolic extract of D. bulbifera bulbs (73.54%) were potent α-amylase inhibitors. Various herbal extracts like Bougainvillea spectabilis and Trigonella foenum-graecum seeds are known as  α-amylase inhibitors showing 29.43% and 59%, respectively [35, 46]. Thus, G. glauca and D. bulbifera exhibited comparatively more efficient α-amylase inhibition.

Bottom Line: Two plants with significant traditional therapeutic potential, namely, Gnidia glauca and Dioscorea bulbifera, were tested for their efficiency to inhibit α-amylase and α-glucosidase.Petroleum ether extract of flower of G. glauca was found to inhibit α-amylase significantly (78.56%).These results provide intense rationale for further in vivo and clinical study.

View Article: PubMed Central - PubMed

Affiliation: Institute of Bioinformatics and Biotechnology, University of Pune, Pune 411007, India.

ABSTRACT
Diabetes is a metabolic disorder affecting about 220 million people worldwide. One of the most critical complications of diabetes is post-prandial hyper-glycemia (PPHG). Glucosidase inhibitor and α-amylase inhibitors are class of compounds that help in managing PPHG. Low-cost herbal treatment is recommended due to their lesser side effect for treatment of diabetes. Two plants with significant traditional therapeutic potential, namely, Gnidia glauca and Dioscorea bulbifera, were tested for their efficiency to inhibit α-amylase and α-glucosidase. Stem, leaf, and flower of G. glauca and bulb of D. bulbifera were sequentially extracted with petroleum ether, ethyl acetate, and methanol as well as separately with 70% ethanol. Petroleum ether extract of flower of G. glauca was found to inhibit α-amylase significantly (78.56%). Extracts were further tested against crude murine pancreatic, small intestinal, and liver glucosidase enzyme which revealed excellent inhibitory properties. α-glucosidase inhibition provided a strong in vitro evidence for confirmation of both G. glauca and D. bulbifera as excellent antidiabetic remedy. This is the first report of its kind that provides a strong biochemical basis for management of type II diabetes using G. glauca and D. bulbifera. These results provide intense rationale for further in vivo and clinical study.

No MeSH data available.


Related in: MedlinePlus