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Antidiabetic Activity of Gnidia glauca and Dioscorea bulbifera: Potent Amylase and Glucosidase Inhibitors.

Ghosh S, Ahire M, Patil S, Jabgunde A, Bhat Dusane M, Joshi BN, Pardesi K, Jachak S, Dhavale DD, Chopade BA - Evid Based Complement Alternat Med (2011)

Bottom Line: Two plants with significant traditional therapeutic potential, namely, Gnidia glauca and Dioscorea bulbifera, were tested for their efficiency to inhibit α-amylase and α-glucosidase.Petroleum ether extract of flower of G. glauca was found to inhibit α-amylase significantly (78.56%).These results provide intense rationale for further in vivo and clinical study.

View Article: PubMed Central - PubMed

Affiliation: Institute of Bioinformatics and Biotechnology, University of Pune, Pune 411007, India.

ABSTRACT
Diabetes is a metabolic disorder affecting about 220 million people worldwide. One of the most critical complications of diabetes is post-prandial hyper-glycemia (PPHG). Glucosidase inhibitor and α-amylase inhibitors are class of compounds that help in managing PPHG. Low-cost herbal treatment is recommended due to their lesser side effect for treatment of diabetes. Two plants with significant traditional therapeutic potential, namely, Gnidia glauca and Dioscorea bulbifera, were tested for their efficiency to inhibit α-amylase and α-glucosidase. Stem, leaf, and flower of G. glauca and bulb of D. bulbifera were sequentially extracted with petroleum ether, ethyl acetate, and methanol as well as separately with 70% ethanol. Petroleum ether extract of flower of G. glauca was found to inhibit α-amylase significantly (78.56%). Extracts were further tested against crude murine pancreatic, small intestinal, and liver glucosidase enzyme which revealed excellent inhibitory properties. α-glucosidase inhibition provided a strong in vitro evidence for confirmation of both G. glauca and D. bulbifera as excellent antidiabetic remedy. This is the first report of its kind that provides a strong biochemical basis for management of type II diabetes using G. glauca and D. bulbifera. These results provide intense rationale for further in vivo and clinical study.

No MeSH data available.


Related in: MedlinePlus

The percent α-glucosidase inhibition by plant extracts. Acarbose is taken as standard inhibitor. The data is indicated as the mean ± SEM; [n = 3].
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fig2: The percent α-glucosidase inhibition by plant extracts. Acarbose is taken as standard inhibitor. The data is indicated as the mean ± SEM; [n = 3].

Mentions: 0.1 Umin−1 of α-glucosidase was taken as 100% enzymatic activity. Petroleum ether extract of D. bulbifera showed strong inhibitory potential with a percentage inhibition of 92.87% as compared to acarbose. Stem, leaf, and flower of G. glauca showed moderate inhibition of 43.54, 21.77 and 51.23%, respectively (Figure 2). Ethyl acetate extract of D. bulbifera bulb was found to be the strongest inhibitor showing an inhibition as high as 99.6%. Similarly, methanol and 70% ethanol extracts of D. bulbifera bulbs exhibited an inhibition of 98.81 and 79.27% which were most significant as compared to others.


Antidiabetic Activity of Gnidia glauca and Dioscorea bulbifera: Potent Amylase and Glucosidase Inhibitors.

Ghosh S, Ahire M, Patil S, Jabgunde A, Bhat Dusane M, Joshi BN, Pardesi K, Jachak S, Dhavale DD, Chopade BA - Evid Based Complement Alternat Med (2011)

The percent α-glucosidase inhibition by plant extracts. Acarbose is taken as standard inhibitor. The data is indicated as the mean ± SEM; [n = 3].
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3140190&req=5

fig2: The percent α-glucosidase inhibition by plant extracts. Acarbose is taken as standard inhibitor. The data is indicated as the mean ± SEM; [n = 3].
Mentions: 0.1 Umin−1 of α-glucosidase was taken as 100% enzymatic activity. Petroleum ether extract of D. bulbifera showed strong inhibitory potential with a percentage inhibition of 92.87% as compared to acarbose. Stem, leaf, and flower of G. glauca showed moderate inhibition of 43.54, 21.77 and 51.23%, respectively (Figure 2). Ethyl acetate extract of D. bulbifera bulb was found to be the strongest inhibitor showing an inhibition as high as 99.6%. Similarly, methanol and 70% ethanol extracts of D. bulbifera bulbs exhibited an inhibition of 98.81 and 79.27% which were most significant as compared to others.

Bottom Line: Two plants with significant traditional therapeutic potential, namely, Gnidia glauca and Dioscorea bulbifera, were tested for their efficiency to inhibit α-amylase and α-glucosidase.Petroleum ether extract of flower of G. glauca was found to inhibit α-amylase significantly (78.56%).These results provide intense rationale for further in vivo and clinical study.

View Article: PubMed Central - PubMed

Affiliation: Institute of Bioinformatics and Biotechnology, University of Pune, Pune 411007, India.

ABSTRACT
Diabetes is a metabolic disorder affecting about 220 million people worldwide. One of the most critical complications of diabetes is post-prandial hyper-glycemia (PPHG). Glucosidase inhibitor and α-amylase inhibitors are class of compounds that help in managing PPHG. Low-cost herbal treatment is recommended due to their lesser side effect for treatment of diabetes. Two plants with significant traditional therapeutic potential, namely, Gnidia glauca and Dioscorea bulbifera, were tested for their efficiency to inhibit α-amylase and α-glucosidase. Stem, leaf, and flower of G. glauca and bulb of D. bulbifera were sequentially extracted with petroleum ether, ethyl acetate, and methanol as well as separately with 70% ethanol. Petroleum ether extract of flower of G. glauca was found to inhibit α-amylase significantly (78.56%). Extracts were further tested against crude murine pancreatic, small intestinal, and liver glucosidase enzyme which revealed excellent inhibitory properties. α-glucosidase inhibition provided a strong in vitro evidence for confirmation of both G. glauca and D. bulbifera as excellent antidiabetic remedy. This is the first report of its kind that provides a strong biochemical basis for management of type II diabetes using G. glauca and D. bulbifera. These results provide intense rationale for further in vivo and clinical study.

No MeSH data available.


Related in: MedlinePlus