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The effects of omega-3 Fatty acids on matrix metalloproteinase-9 production and cell migration in human immune cells: implications for multiple sclerosis.

Shinto L, Marracci G, Bumgarner L, Yadav V - Autoimmune Dis (2011)

Bottom Line: Matrix metalloproteinase-9 (MMP-9) is associated with BBB disruption and subsequent T cell migration into the CNS.The aim of this paper was to evaluate the effects of omega-3 fatty acids on MMP-9 levels and T cell migration.EPA and DHA significantly decreased MMP-9 protein levels, MMP-9 activity, and significantly inhibited human T cell migration.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurology, Oregon Health & Science University, 3181 SW Sam Jackson Park Road, CR 120, Portland, OR 97239, USA.

ABSTRACT
In multiple sclerosis (MS), compromised blood-brain barrier (BBB) integrity contributes to inflammatory T cell migration into the central nervous system. Matrix metalloproteinase-9 (MMP-9) is associated with BBB disruption and subsequent T cell migration into the CNS. The aim of this paper was to evaluate the effects of omega-3 fatty acids on MMP-9 levels and T cell migration. Peripheral blood mononuclear cells (PBMC) from healthy controls were pretreated with two types of omega-3 fatty acids, eicosapentaenoic acid (EPA), and docosahexaenoic acid (DHA). Cell supernatants were used to determine MMP-9 protein and activity levels. Jurkat cells were pretreated with EPA and DHA and were added to fibronectin-coated transwells to measure T cell migration. EPA and DHA significantly decreased MMP-9 protein levels, MMP-9 activity, and significantly inhibited human T cell migration. The data suggest that omega-3 fatty acids may benefit patients with multiple sclerosis by modulating immune cell production of MMP-9.

No MeSH data available.


Related in: MedlinePlus

Jurkat cell migration is decreased following treatment with DHA or EPA when compared to an untreated control (100% migration). MMP-9 activity from Jurkat cell supernatants is decreased following treatment with DHA or EPA when compared to an untreated control (100% activity). Supernatants were collected from the cultures undergoing migration to determine MMP-9 activity by gelatin substrate zymography. Jurkat cells were incubated for 20 hr in the presence of 10, 30, and 100 μg/mL of DHA or EPA. OA (30 μg/mL) served as an oil control for cell migration. Migration assays were performed three separate times, representative data is displayed. *Significant decrease in migration compared to control (P ≤ 0.05). **Significant decrease in Jurkat cell migration and MMP-9 activity compared to control (P ≤ 0.05).
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fig3: Jurkat cell migration is decreased following treatment with DHA or EPA when compared to an untreated control (100% migration). MMP-9 activity from Jurkat cell supernatants is decreased following treatment with DHA or EPA when compared to an untreated control (100% activity). Supernatants were collected from the cultures undergoing migration to determine MMP-9 activity by gelatin substrate zymography. Jurkat cells were incubated for 20 hr in the presence of 10, 30, and 100 μg/mL of DHA or EPA. OA (30 μg/mL) served as an oil control for cell migration. Migration assays were performed three separate times, representative data is displayed. *Significant decrease in migration compared to control (P ≤ 0.05). **Significant decrease in Jurkat cell migration and MMP-9 activity compared to control (P ≤ 0.05).

Mentions: Coincubation of human Jurkat T cells with DHA and EPA reduced their migratory capacity across a pseudo-blood brain barrier in a dose dependent manner (Figures 3(a) and 3(b)). DHA at 30 μg/mL reduced Jurkat cell migration by 46.2% and at 100 μg/mL by 83.9% (P ≤ 0.05 for both). Culture supernatants collected at the end of the migration period were subjected to electrophoresis and gelatin substrate zymography to assess MMP-9 activity. Corresponding to the effect on migratory capacity, DHA at 30 μg/mL reduced MMP-9 activity by 36.3% and at 100 μg/mL by 97.6% [P < 0.05 for both; Figure 3(a)]. Similarly, EPA treatment of Jurkat cell cultures significantly reduced the ability of Jurkat cells to cross a fibronectin barrier with a corresponding reduction in MMP-9 activity. EPA at 10 μg/mL reduced Jurkat cell migration by 46.9%, at 30 μg/mL by 51.8%, and at 100 μg/mL by 83.1% (P ≤ 0.05 for all). EPA at 30 μg/mL reduced MMP-9 activity by 69.6% and at 100 μg/mL by 88.3% [P ≤ 0.05 for both; Figure 3(b)]. OA (oil control) at 30 μg/mL reduced Jurkat cell migration by 5.5% (P = 0.66), MMP-9 activity was not assessed in cells treated with OA.


The effects of omega-3 Fatty acids on matrix metalloproteinase-9 production and cell migration in human immune cells: implications for multiple sclerosis.

Shinto L, Marracci G, Bumgarner L, Yadav V - Autoimmune Dis (2011)

Jurkat cell migration is decreased following treatment with DHA or EPA when compared to an untreated control (100% migration). MMP-9 activity from Jurkat cell supernatants is decreased following treatment with DHA or EPA when compared to an untreated control (100% activity). Supernatants were collected from the cultures undergoing migration to determine MMP-9 activity by gelatin substrate zymography. Jurkat cells were incubated for 20 hr in the presence of 10, 30, and 100 μg/mL of DHA or EPA. OA (30 μg/mL) served as an oil control for cell migration. Migration assays were performed three separate times, representative data is displayed. *Significant decrease in migration compared to control (P ≤ 0.05). **Significant decrease in Jurkat cell migration and MMP-9 activity compared to control (P ≤ 0.05).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3140187&req=5

fig3: Jurkat cell migration is decreased following treatment with DHA or EPA when compared to an untreated control (100% migration). MMP-9 activity from Jurkat cell supernatants is decreased following treatment with DHA or EPA when compared to an untreated control (100% activity). Supernatants were collected from the cultures undergoing migration to determine MMP-9 activity by gelatin substrate zymography. Jurkat cells were incubated for 20 hr in the presence of 10, 30, and 100 μg/mL of DHA or EPA. OA (30 μg/mL) served as an oil control for cell migration. Migration assays were performed three separate times, representative data is displayed. *Significant decrease in migration compared to control (P ≤ 0.05). **Significant decrease in Jurkat cell migration and MMP-9 activity compared to control (P ≤ 0.05).
Mentions: Coincubation of human Jurkat T cells with DHA and EPA reduced their migratory capacity across a pseudo-blood brain barrier in a dose dependent manner (Figures 3(a) and 3(b)). DHA at 30 μg/mL reduced Jurkat cell migration by 46.2% and at 100 μg/mL by 83.9% (P ≤ 0.05 for both). Culture supernatants collected at the end of the migration period were subjected to electrophoresis and gelatin substrate zymography to assess MMP-9 activity. Corresponding to the effect on migratory capacity, DHA at 30 μg/mL reduced MMP-9 activity by 36.3% and at 100 μg/mL by 97.6% [P < 0.05 for both; Figure 3(a)]. Similarly, EPA treatment of Jurkat cell cultures significantly reduced the ability of Jurkat cells to cross a fibronectin barrier with a corresponding reduction in MMP-9 activity. EPA at 10 μg/mL reduced Jurkat cell migration by 46.9%, at 30 μg/mL by 51.8%, and at 100 μg/mL by 83.1% (P ≤ 0.05 for all). EPA at 30 μg/mL reduced MMP-9 activity by 69.6% and at 100 μg/mL by 88.3% [P ≤ 0.05 for both; Figure 3(b)]. OA (oil control) at 30 μg/mL reduced Jurkat cell migration by 5.5% (P = 0.66), MMP-9 activity was not assessed in cells treated with OA.

Bottom Line: Matrix metalloproteinase-9 (MMP-9) is associated with BBB disruption and subsequent T cell migration into the CNS.The aim of this paper was to evaluate the effects of omega-3 fatty acids on MMP-9 levels and T cell migration.EPA and DHA significantly decreased MMP-9 protein levels, MMP-9 activity, and significantly inhibited human T cell migration.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurology, Oregon Health & Science University, 3181 SW Sam Jackson Park Road, CR 120, Portland, OR 97239, USA.

ABSTRACT
In multiple sclerosis (MS), compromised blood-brain barrier (BBB) integrity contributes to inflammatory T cell migration into the central nervous system. Matrix metalloproteinase-9 (MMP-9) is associated with BBB disruption and subsequent T cell migration into the CNS. The aim of this paper was to evaluate the effects of omega-3 fatty acids on MMP-9 levels and T cell migration. Peripheral blood mononuclear cells (PBMC) from healthy controls were pretreated with two types of omega-3 fatty acids, eicosapentaenoic acid (EPA), and docosahexaenoic acid (DHA). Cell supernatants were used to determine MMP-9 protein and activity levels. Jurkat cells were pretreated with EPA and DHA and were added to fibronectin-coated transwells to measure T cell migration. EPA and DHA significantly decreased MMP-9 protein levels, MMP-9 activity, and significantly inhibited human T cell migration. The data suggest that omega-3 fatty acids may benefit patients with multiple sclerosis by modulating immune cell production of MMP-9.

No MeSH data available.


Related in: MedlinePlus