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The effects of omega-3 Fatty acids on matrix metalloproteinase-9 production and cell migration in human immune cells: implications for multiple sclerosis.

Shinto L, Marracci G, Bumgarner L, Yadav V - Autoimmune Dis (2011)

Bottom Line: Matrix metalloproteinase-9 (MMP-9) is associated with BBB disruption and subsequent T cell migration into the CNS.The aim of this paper was to evaluate the effects of omega-3 fatty acids on MMP-9 levels and T cell migration.EPA and DHA significantly decreased MMP-9 protein levels, MMP-9 activity, and significantly inhibited human T cell migration.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurology, Oregon Health & Science University, 3181 SW Sam Jackson Park Road, CR 120, Portland, OR 97239, USA.

ABSTRACT
In multiple sclerosis (MS), compromised blood-brain barrier (BBB) integrity contributes to inflammatory T cell migration into the central nervous system. Matrix metalloproteinase-9 (MMP-9) is associated with BBB disruption and subsequent T cell migration into the CNS. The aim of this paper was to evaluate the effects of omega-3 fatty acids on MMP-9 levels and T cell migration. Peripheral blood mononuclear cells (PBMC) from healthy controls were pretreated with two types of omega-3 fatty acids, eicosapentaenoic acid (EPA), and docosahexaenoic acid (DHA). Cell supernatants were used to determine MMP-9 protein and activity levels. Jurkat cells were pretreated with EPA and DHA and were added to fibronectin-coated transwells to measure T cell migration. EPA and DHA significantly decreased MMP-9 protein levels, MMP-9 activity, and significantly inhibited human T cell migration. The data suggest that omega-3 fatty acids may benefit patients with multiple sclerosis by modulating immune cell production of MMP-9.

No MeSH data available.


Related in: MedlinePlus

Mean MMP-9 protein levels secreted from PBMC. PBMC from three different normal controls. MMP-9 levels secreted from ConA-stimulated PBMC is decreased following treatment with DHA and EPA when compared to an untreated ConA-stimulated PBMC control. OA served as an oil control and showed no significant decrease in MMP-9 levels at any concentration. PBMC were pretreated for 2 hr in the presence of 10, 25, and 50 μg/mL of one of the following, DHA, EPA, or OA. Cells were then stimulated with ConA and supernatants were collected to determine MMP-9 levels by ELISA. Error bars represent standard error (SE). *Significant decrease compared to control (P ≤ 0.05).
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fig1: Mean MMP-9 protein levels secreted from PBMC. PBMC from three different normal controls. MMP-9 levels secreted from ConA-stimulated PBMC is decreased following treatment with DHA and EPA when compared to an untreated ConA-stimulated PBMC control. OA served as an oil control and showed no significant decrease in MMP-9 levels at any concentration. PBMC were pretreated for 2 hr in the presence of 10, 25, and 50 μg/mL of one of the following, DHA, EPA, or OA. Cells were then stimulated with ConA and supernatants were collected to determine MMP-9 levels by ELISA. Error bars represent standard error (SE). *Significant decrease compared to control (P ≤ 0.05).

Mentions: Figure 1 shows the effects of DHA, EPA, and the oil control OA on MMP-9 protein levels. DHA at 10 μg/mL decreased mean MMP-9 levels to 85.5 ng/mL (SE ± 10.9; P = 1.0), at 25 μg/mL decreased mean MMP-9 levels to 41.5 ng/mL (SE ± 7.5; P = 0.29), and at 50 μg/mL deceased MMP-9 levels to non-detectable (P = 0.05). EPA at 10 μg/mL decreased mean MMP-9 levels to 50.9 ng/mL (SE ± 9.2; P = 0.61), at 25 μg/mL decreased mean MMP-9 levels to 4.4 ng/mL (SE ± 4.4; P = 0.05), and at 50 μg/mL decreased MMP-9 levels to non-detectable (P = 0.04). There was no significant decrease in MMP-9 levels for any of the OA concentrations (P = 1.0 for all concentrations).


The effects of omega-3 Fatty acids on matrix metalloproteinase-9 production and cell migration in human immune cells: implications for multiple sclerosis.

Shinto L, Marracci G, Bumgarner L, Yadav V - Autoimmune Dis (2011)

Mean MMP-9 protein levels secreted from PBMC. PBMC from three different normal controls. MMP-9 levels secreted from ConA-stimulated PBMC is decreased following treatment with DHA and EPA when compared to an untreated ConA-stimulated PBMC control. OA served as an oil control and showed no significant decrease in MMP-9 levels at any concentration. PBMC were pretreated for 2 hr in the presence of 10, 25, and 50 μg/mL of one of the following, DHA, EPA, or OA. Cells were then stimulated with ConA and supernatants were collected to determine MMP-9 levels by ELISA. Error bars represent standard error (SE). *Significant decrease compared to control (P ≤ 0.05).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3140187&req=5

fig1: Mean MMP-9 protein levels secreted from PBMC. PBMC from three different normal controls. MMP-9 levels secreted from ConA-stimulated PBMC is decreased following treatment with DHA and EPA when compared to an untreated ConA-stimulated PBMC control. OA served as an oil control and showed no significant decrease in MMP-9 levels at any concentration. PBMC were pretreated for 2 hr in the presence of 10, 25, and 50 μg/mL of one of the following, DHA, EPA, or OA. Cells were then stimulated with ConA and supernatants were collected to determine MMP-9 levels by ELISA. Error bars represent standard error (SE). *Significant decrease compared to control (P ≤ 0.05).
Mentions: Figure 1 shows the effects of DHA, EPA, and the oil control OA on MMP-9 protein levels. DHA at 10 μg/mL decreased mean MMP-9 levels to 85.5 ng/mL (SE ± 10.9; P = 1.0), at 25 μg/mL decreased mean MMP-9 levels to 41.5 ng/mL (SE ± 7.5; P = 0.29), and at 50 μg/mL deceased MMP-9 levels to non-detectable (P = 0.05). EPA at 10 μg/mL decreased mean MMP-9 levels to 50.9 ng/mL (SE ± 9.2; P = 0.61), at 25 μg/mL decreased mean MMP-9 levels to 4.4 ng/mL (SE ± 4.4; P = 0.05), and at 50 μg/mL decreased MMP-9 levels to non-detectable (P = 0.04). There was no significant decrease in MMP-9 levels for any of the OA concentrations (P = 1.0 for all concentrations).

Bottom Line: Matrix metalloproteinase-9 (MMP-9) is associated with BBB disruption and subsequent T cell migration into the CNS.The aim of this paper was to evaluate the effects of omega-3 fatty acids on MMP-9 levels and T cell migration.EPA and DHA significantly decreased MMP-9 protein levels, MMP-9 activity, and significantly inhibited human T cell migration.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurology, Oregon Health & Science University, 3181 SW Sam Jackson Park Road, CR 120, Portland, OR 97239, USA.

ABSTRACT
In multiple sclerosis (MS), compromised blood-brain barrier (BBB) integrity contributes to inflammatory T cell migration into the central nervous system. Matrix metalloproteinase-9 (MMP-9) is associated with BBB disruption and subsequent T cell migration into the CNS. The aim of this paper was to evaluate the effects of omega-3 fatty acids on MMP-9 levels and T cell migration. Peripheral blood mononuclear cells (PBMC) from healthy controls were pretreated with two types of omega-3 fatty acids, eicosapentaenoic acid (EPA), and docosahexaenoic acid (DHA). Cell supernatants were used to determine MMP-9 protein and activity levels. Jurkat cells were pretreated with EPA and DHA and were added to fibronectin-coated transwells to measure T cell migration. EPA and DHA significantly decreased MMP-9 protein levels, MMP-9 activity, and significantly inhibited human T cell migration. The data suggest that omega-3 fatty acids may benefit patients with multiple sclerosis by modulating immune cell production of MMP-9.

No MeSH data available.


Related in: MedlinePlus