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Diuretic and natriuretic activity of two mistletoe species in rats.

Jadhav N, Patil CR, Chaudhari KB, Wagh JP, Surana SJ, Jadhav RB - Pharmacognosy Res (2010)

Bottom Line: It was found that while VA mimics the furosemide pattern, HE demonstrated a dose-dependent increase in diuresis, along with an increase in potassium-sparing effects.It was also found that in different combinations, these phytochemicals differed in the way they influenced the electrolyte excretion.A higher content of polyphenolics in association with lower triterpenoid content was found to favor potassium-sparing effects.

View Article: PubMed Central - PubMed

Affiliation: Bioecology Laboratory, Department of Pharmacognosy, R. C. Patel Institute of Pharmaceutical Education and Research, Shirpur - 425 405, India.

ABSTRACT
In different cultural groups, the hemiparasitic plants of the families Loranthaceae and Viscaceae (mistletoes) are frequently used in the treatment of hypertension and/or as diuretic agents. However, it remains unclear as to what commonality makes them diuretic agents or a remedy for hypertension. In this article, the diuretic activity of methanol extracts of Viscum articulatum (VA) Burm. f. and Helicanthus elastica (HE) (Ders.) Dans. in rats is reported. The extracts were administered orally at doses of 100, 200 and 400 mg/kg to rats that had been fasted and deprived of water for 18 hours. Investigations were carried out for diuretic, saluretic and natriuretic effects. The polyphenolic and triterpenoid contents were determined quantitatively using chemical assays and high performance liquid chromatography (HPLC) analysis, respectively. The extracts of VA and HE demonstrated significant and dose-dependent diuretic activity in rats. It was found that while VA mimics the furosemide pattern, HE demonstrated a dose-dependent increase in diuresis, along with an increase in potassium-sparing effects. Phytochemical analysis revealed that polyphenolics and triterpenoids, such as oleanolic acid and lupeol, are the major phytochemicals involved. It was also found that in different combinations, these phytochemicals differed in the way they influenced the electrolyte excretion. A higher content of polyphenolics in association with lower triterpenoid content was found to favor potassium-sparing effects.

No MeSH data available.


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HPLC analysis of methanol extract of Helicanthus elastica (a) and marker compound lupeol (b) unidentified triterpenoid, UIT
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Figure 4: HPLC analysis of methanol extract of Helicanthus elastica (a) and marker compound lupeol (b) unidentified triterpenoid, UIT

Mentions: The sodium and potassium levels in HE and VA extracts were 94.56 mMol/L, 40.51 mMol/L; and 131 mMol/L, 51.92 mMol/L, respectively. Further, the quantitative chemical assays revealed a preponderance of polyphenolic compounds in the HE extract in comparison with the VA extract. The gross phenolic content in HE and VA was 5.02 ± 0.61 g % and 0.39 ± 0.02 g %, respectively [Table 2]. Among the polyphenolic compounds, flavonoids constituted a major part, followed by proanthocyanidin compounds. The results of the HPLC analysis show that oleanolic acid is the major triterpenoid of the VA extract, with a small betulinic acid content [Figure 3 and Table 2]. The results further indicate that neither oleanolic acid nor betulinic acid is present in HE and that lupeol is the major triterpenoid. However, one additional peak representing an unidentified triterpenoid was also detected [Figure 4]. Quantification further suggests that, in contrast to the polyphenolics, the triterpenoid allocation is the opposite, with the VA extract having a 19% higher triterpenoid content in comparison with the HE extract [Table 2].


Diuretic and natriuretic activity of two mistletoe species in rats.

Jadhav N, Patil CR, Chaudhari KB, Wagh JP, Surana SJ, Jadhav RB - Pharmacognosy Res (2010)

HPLC analysis of methanol extract of Helicanthus elastica (a) and marker compound lupeol (b) unidentified triterpenoid, UIT
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3140130&req=5

Figure 4: HPLC analysis of methanol extract of Helicanthus elastica (a) and marker compound lupeol (b) unidentified triterpenoid, UIT
Mentions: The sodium and potassium levels in HE and VA extracts were 94.56 mMol/L, 40.51 mMol/L; and 131 mMol/L, 51.92 mMol/L, respectively. Further, the quantitative chemical assays revealed a preponderance of polyphenolic compounds in the HE extract in comparison with the VA extract. The gross phenolic content in HE and VA was 5.02 ± 0.61 g % and 0.39 ± 0.02 g %, respectively [Table 2]. Among the polyphenolic compounds, flavonoids constituted a major part, followed by proanthocyanidin compounds. The results of the HPLC analysis show that oleanolic acid is the major triterpenoid of the VA extract, with a small betulinic acid content [Figure 3 and Table 2]. The results further indicate that neither oleanolic acid nor betulinic acid is present in HE and that lupeol is the major triterpenoid. However, one additional peak representing an unidentified triterpenoid was also detected [Figure 4]. Quantification further suggests that, in contrast to the polyphenolics, the triterpenoid allocation is the opposite, with the VA extract having a 19% higher triterpenoid content in comparison with the HE extract [Table 2].

Bottom Line: It was found that while VA mimics the furosemide pattern, HE demonstrated a dose-dependent increase in diuresis, along with an increase in potassium-sparing effects.It was also found that in different combinations, these phytochemicals differed in the way they influenced the electrolyte excretion.A higher content of polyphenolics in association with lower triterpenoid content was found to favor potassium-sparing effects.

View Article: PubMed Central - PubMed

Affiliation: Bioecology Laboratory, Department of Pharmacognosy, R. C. Patel Institute of Pharmaceutical Education and Research, Shirpur - 425 405, India.

ABSTRACT
In different cultural groups, the hemiparasitic plants of the families Loranthaceae and Viscaceae (mistletoes) are frequently used in the treatment of hypertension and/or as diuretic agents. However, it remains unclear as to what commonality makes them diuretic agents or a remedy for hypertension. In this article, the diuretic activity of methanol extracts of Viscum articulatum (VA) Burm. f. and Helicanthus elastica (HE) (Ders.) Dans. in rats is reported. The extracts were administered orally at doses of 100, 200 and 400 mg/kg to rats that had been fasted and deprived of water for 18 hours. Investigations were carried out for diuretic, saluretic and natriuretic effects. The polyphenolic and triterpenoid contents were determined quantitatively using chemical assays and high performance liquid chromatography (HPLC) analysis, respectively. The extracts of VA and HE demonstrated significant and dose-dependent diuretic activity in rats. It was found that while VA mimics the furosemide pattern, HE demonstrated a dose-dependent increase in diuresis, along with an increase in potassium-sparing effects. Phytochemical analysis revealed that polyphenolics and triterpenoids, such as oleanolic acid and lupeol, are the major phytochemicals involved. It was also found that in different combinations, these phytochemicals differed in the way they influenced the electrolyte excretion. A higher content of polyphenolics in association with lower triterpenoid content was found to favor potassium-sparing effects.

No MeSH data available.


Related in: MedlinePlus