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Broad-spectrum sun-protective action of Porphyra-334 derived from Porphyra vietnamensis.

Bhatia S, Sharma K, Namdeo AG, Chaugule BB, Kavale M, Nanda S - Pharmacognosy Res (2010)

Bottom Line: Stability studies were performed under different storage and pH conditions.Ultimately a sunscreen formulation was developed and its potential against marketed Aloe vera gel was evaluated by in vitro sunscreen protection method.It was observed that sunscreen potential of Porphyra-334 was 5.11-fold greater than that of the marketed Aloe vera gel preparation.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacognosy, Poona College of Pharmacy, Bharati Vidyapeeth University, Pune, India.

ABSTRACT
There are enormous UV-protective compounds present in the current world market, out of which 98% give protection against UV-B range and the remaining 2% are potent against far UV-A range only. Furthermore, these synthetic compounds have various problems related to photo-stability and cross-stability. There is a vital need of sunscreen agents that will remain stable for prolonged periods and provide broad-spectrum protection against harmful UV range. The Indian Ocean contains large amounts of macro-algae which synthesize varied amount of mycosporine amino acids, "sun-protective compounds" by shikmic acid pathway. In the present study, we have evaluated the sunscreen protection provided by Porphyra-334, a mycosporine amino acid isolated from Indian sp. of Porphyra. Furthermore, the isolated compound was detected by high performance thin layer chromatography (HPTLC) fingerprinting, high performance liquid chromatography (HPLC) and ultraviolet (UV), whereas nuclear magnetic resonance (NMR) spectroscopy and infrared spectrometry were used for its structural characterization. Stability studies were performed under different storage and pH conditions. Ultimately a sunscreen formulation was developed and its potential against marketed Aloe vera gel was evaluated by in vitro sunscreen protection method. It was observed that sunscreen potential of Porphyra-334 was 5.11-fold greater than that of the marketed Aloe vera gel preparation.

No MeSH data available.


Stability studies of Porphyra-334 with different storage periods
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Figure 4: Stability studies of Porphyra-334 with different storage periods

Mentions: In the present study, MAA, known to be present in macro-algae of Porphyra vietnamensis, was isolated and detected by UV (334 nm), HPTLC (Rf- 0.43-0.53) fingerprinting and HPLC (RT 4.9) analysis [Figures 1–3]. In Figure 1, methanolic aliquot showed the sharp peak at 334 nm with an extinction coefficient of 43200 M-1cm-1. Melting temperature of Porphyra-334 (164°C-165°C) was studied by DSC (differential scanning calorimeter). Structural characterization was done on the basis of 13C-NMR and 1H-NMR[Table 2], whereas IR studies gave the range of 3100, 1520, 1670, 1330 and 1100 cm-1. Thereafter, stability studies of gel-loaded drug were performed under different pH and storage conditions simultaneously [Figures 4 and 5].


Broad-spectrum sun-protective action of Porphyra-334 derived from Porphyra vietnamensis.

Bhatia S, Sharma K, Namdeo AG, Chaugule BB, Kavale M, Nanda S - Pharmacognosy Res (2010)

Stability studies of Porphyra-334 with different storage periods
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3140129&req=5

Figure 4: Stability studies of Porphyra-334 with different storage periods
Mentions: In the present study, MAA, known to be present in macro-algae of Porphyra vietnamensis, was isolated and detected by UV (334 nm), HPTLC (Rf- 0.43-0.53) fingerprinting and HPLC (RT 4.9) analysis [Figures 1–3]. In Figure 1, methanolic aliquot showed the sharp peak at 334 nm with an extinction coefficient of 43200 M-1cm-1. Melting temperature of Porphyra-334 (164°C-165°C) was studied by DSC (differential scanning calorimeter). Structural characterization was done on the basis of 13C-NMR and 1H-NMR[Table 2], whereas IR studies gave the range of 3100, 1520, 1670, 1330 and 1100 cm-1. Thereafter, stability studies of gel-loaded drug were performed under different pH and storage conditions simultaneously [Figures 4 and 5].

Bottom Line: Stability studies were performed under different storage and pH conditions.Ultimately a sunscreen formulation was developed and its potential against marketed Aloe vera gel was evaluated by in vitro sunscreen protection method.It was observed that sunscreen potential of Porphyra-334 was 5.11-fold greater than that of the marketed Aloe vera gel preparation.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacognosy, Poona College of Pharmacy, Bharati Vidyapeeth University, Pune, India.

ABSTRACT
There are enormous UV-protective compounds present in the current world market, out of which 98% give protection against UV-B range and the remaining 2% are potent against far UV-A range only. Furthermore, these synthetic compounds have various problems related to photo-stability and cross-stability. There is a vital need of sunscreen agents that will remain stable for prolonged periods and provide broad-spectrum protection against harmful UV range. The Indian Ocean contains large amounts of macro-algae which synthesize varied amount of mycosporine amino acids, "sun-protective compounds" by shikmic acid pathway. In the present study, we have evaluated the sunscreen protection provided by Porphyra-334, a mycosporine amino acid isolated from Indian sp. of Porphyra. Furthermore, the isolated compound was detected by high performance thin layer chromatography (HPTLC) fingerprinting, high performance liquid chromatography (HPLC) and ultraviolet (UV), whereas nuclear magnetic resonance (NMR) spectroscopy and infrared spectrometry were used for its structural characterization. Stability studies were performed under different storage and pH conditions. Ultimately a sunscreen formulation was developed and its potential against marketed Aloe vera gel was evaluated by in vitro sunscreen protection method. It was observed that sunscreen potential of Porphyra-334 was 5.11-fold greater than that of the marketed Aloe vera gel preparation.

No MeSH data available.