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Broad-spectrum sun-protective action of Porphyra-334 derived from Porphyra vietnamensis.

Bhatia S, Sharma K, Namdeo AG, Chaugule BB, Kavale M, Nanda S - Pharmacognosy Res (2010)

Bottom Line: Stability studies were performed under different storage and pH conditions.Ultimately a sunscreen formulation was developed and its potential against marketed Aloe vera gel was evaluated by in vitro sunscreen protection method.It was observed that sunscreen potential of Porphyra-334 was 5.11-fold greater than that of the marketed Aloe vera gel preparation.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacognosy, Poona College of Pharmacy, Bharati Vidyapeeth University, Pune, India.

ABSTRACT
There are enormous UV-protective compounds present in the current world market, out of which 98% give protection against UV-B range and the remaining 2% are potent against far UV-A range only. Furthermore, these synthetic compounds have various problems related to photo-stability and cross-stability. There is a vital need of sunscreen agents that will remain stable for prolonged periods and provide broad-spectrum protection against harmful UV range. The Indian Ocean contains large amounts of macro-algae which synthesize varied amount of mycosporine amino acids, "sun-protective compounds" by shikmic acid pathway. In the present study, we have evaluated the sunscreen protection provided by Porphyra-334, a mycosporine amino acid isolated from Indian sp. of Porphyra. Furthermore, the isolated compound was detected by high performance thin layer chromatography (HPTLC) fingerprinting, high performance liquid chromatography (HPLC) and ultraviolet (UV), whereas nuclear magnetic resonance (NMR) spectroscopy and infrared spectrometry were used for its structural characterization. Stability studies were performed under different storage and pH conditions. Ultimately a sunscreen formulation was developed and its potential against marketed Aloe vera gel was evaluated by in vitro sunscreen protection method. It was observed that sunscreen potential of Porphyra-334 was 5.11-fold greater than that of the marketed Aloe vera gel preparation.

No MeSH data available.


Detection of mycosporine-like amino acid (334 nm) by ultraviolet from the methanolic aliquot of Porphyra
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Figure 1: Detection of mycosporine-like amino acid (334 nm) by ultraviolet from the methanolic aliquot of Porphyra

Mentions: Ten grams of dried algae was powdered. Extraction of the powder was carried out with a mixture of methanol-water (80:20, v/v), followed by CH2Cl2. The aqueous phase was lyophilized till dryness, to yield 5.1 g of a pale yellow powder. An aliquot of the powder was re-dissolved in 80% MeOH and checked for its UV absorbance. A sharp peak with an extinction coefficient of 42,300 M-1cm-1 was obtained at 334 nm [Figure 1]. The resulting powder was chromatographed on a silica gel column, using a gradient condition ranging from 20% MeOH and 80% EtOH to 80% MeOH and 20% EtOH, to yield 400 mg of a pale yellow powder. The collected fractions were evaporated and then lyophilized till dryness to yield 50 mg of colorless powder. Purity of the isolated compound was confirmed by HPLC analysis, performed on a Jasco 900 instrument, and 5 µL of isolated fraction was loaded onto ODS (5 µm; Inertsil) column (150 × 4.6 mm). Acetonitrile:Water (45:55) was eluted at a flow rate of 1 mL/min, which showed a single sharp peak with a retention time of 4.9 minutes. The analysis was carried out on highly polar water-soluble compound that absorbs strongly in the UV-A region. Further analysis was done by HPTLC to know the exact position of isolated compound on the chromatogram. The spotting device was Linomat IV Automatic Sampler (Camag, Muttenz, Switzerland), 100 µL syringe (Hamilton Bonaduz, Switzerland). The TLC chamber; glass twin-trough chamber (20 × 10 × 4 cm) (Camag); TLC Scanner 3 linked to WINCATS software (Camag) as densitometer; the HPTLC plates measuring 20 × 10 cm, 0.2 mm thickness, pre-coated with silica gel 60 F254 (E. Merck Kga A, Cat. no. 1.05548, Darmstadt, Germany), were used. Structural characterization was done with the help of NMR spectroscopy and infrared spectrometry. Stability studies of isolated fraction were performed with different storage periods and pH conditions by using calibrated pH meter.


Broad-spectrum sun-protective action of Porphyra-334 derived from Porphyra vietnamensis.

Bhatia S, Sharma K, Namdeo AG, Chaugule BB, Kavale M, Nanda S - Pharmacognosy Res (2010)

Detection of mycosporine-like amino acid (334 nm) by ultraviolet from the methanolic aliquot of Porphyra
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3140129&req=5

Figure 1: Detection of mycosporine-like amino acid (334 nm) by ultraviolet from the methanolic aliquot of Porphyra
Mentions: Ten grams of dried algae was powdered. Extraction of the powder was carried out with a mixture of methanol-water (80:20, v/v), followed by CH2Cl2. The aqueous phase was lyophilized till dryness, to yield 5.1 g of a pale yellow powder. An aliquot of the powder was re-dissolved in 80% MeOH and checked for its UV absorbance. A sharp peak with an extinction coefficient of 42,300 M-1cm-1 was obtained at 334 nm [Figure 1]. The resulting powder was chromatographed on a silica gel column, using a gradient condition ranging from 20% MeOH and 80% EtOH to 80% MeOH and 20% EtOH, to yield 400 mg of a pale yellow powder. The collected fractions were evaporated and then lyophilized till dryness to yield 50 mg of colorless powder. Purity of the isolated compound was confirmed by HPLC analysis, performed on a Jasco 900 instrument, and 5 µL of isolated fraction was loaded onto ODS (5 µm; Inertsil) column (150 × 4.6 mm). Acetonitrile:Water (45:55) was eluted at a flow rate of 1 mL/min, which showed a single sharp peak with a retention time of 4.9 minutes. The analysis was carried out on highly polar water-soluble compound that absorbs strongly in the UV-A region. Further analysis was done by HPTLC to know the exact position of isolated compound on the chromatogram. The spotting device was Linomat IV Automatic Sampler (Camag, Muttenz, Switzerland), 100 µL syringe (Hamilton Bonaduz, Switzerland). The TLC chamber; glass twin-trough chamber (20 × 10 × 4 cm) (Camag); TLC Scanner 3 linked to WINCATS software (Camag) as densitometer; the HPTLC plates measuring 20 × 10 cm, 0.2 mm thickness, pre-coated with silica gel 60 F254 (E. Merck Kga A, Cat. no. 1.05548, Darmstadt, Germany), were used. Structural characterization was done with the help of NMR spectroscopy and infrared spectrometry. Stability studies of isolated fraction were performed with different storage periods and pH conditions by using calibrated pH meter.

Bottom Line: Stability studies were performed under different storage and pH conditions.Ultimately a sunscreen formulation was developed and its potential against marketed Aloe vera gel was evaluated by in vitro sunscreen protection method.It was observed that sunscreen potential of Porphyra-334 was 5.11-fold greater than that of the marketed Aloe vera gel preparation.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacognosy, Poona College of Pharmacy, Bharati Vidyapeeth University, Pune, India.

ABSTRACT
There are enormous UV-protective compounds present in the current world market, out of which 98% give protection against UV-B range and the remaining 2% are potent against far UV-A range only. Furthermore, these synthetic compounds have various problems related to photo-stability and cross-stability. There is a vital need of sunscreen agents that will remain stable for prolonged periods and provide broad-spectrum protection against harmful UV range. The Indian Ocean contains large amounts of macro-algae which synthesize varied amount of mycosporine amino acids, "sun-protective compounds" by shikmic acid pathway. In the present study, we have evaluated the sunscreen protection provided by Porphyra-334, a mycosporine amino acid isolated from Indian sp. of Porphyra. Furthermore, the isolated compound was detected by high performance thin layer chromatography (HPTLC) fingerprinting, high performance liquid chromatography (HPLC) and ultraviolet (UV), whereas nuclear magnetic resonance (NMR) spectroscopy and infrared spectrometry were used for its structural characterization. Stability studies were performed under different storage and pH conditions. Ultimately a sunscreen formulation was developed and its potential against marketed Aloe vera gel was evaluated by in vitro sunscreen protection method. It was observed that sunscreen potential of Porphyra-334 was 5.11-fold greater than that of the marketed Aloe vera gel preparation.

No MeSH data available.