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Bioactivity of Syzygium jambos methanolic extracts: Antibacterial activity and toxicity.

Mohanty S, Cock IE - Pharmacognosy Res (2010)

Bottom Line: Methanol extracts from S. jambos leaves were tested for antimicrobial activity and toxicity.The leaf extract also proved to be toxic in the Artemia franciscana bioassay, with a 48-h LC(50) of 387.9 ± 38.8 µg/mL, making it slightly more toxic than Mevinphos (505.3± 37.7 µg/mL) and approximately 5-fold less toxic than potassium dichromate (80.4 ± 4.3 µg/mL).Whilst potassium dichromate's LC(50) remained constant across the 72-hour test period (24-h LC(50), 86.3 ± 5.1; 72-h LC(50), 77.9 ± 4.9), the extract and Mevinphos LC(50) values decreased by 72 hours (87.0 ± 11.3 µg/mL and 103.9 ± 12.8 µg/mL, respectively), indicating their similar levels of toxicity in the assay.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomolecular and Physical Sciences, Nathan Campus, Griffith University, 170 Kessels Rd., Nathan, Brisbane, Queensland 4111, Australia.

ABSTRACT
Methanol extracts from S. jambos leaves were tested for antimicrobial activity and toxicity. S. jambos leaf extract inhibited the growth of 4 of the 14 bacteria tested (29%). Both gram-positive and gram-negative bacterial growths were inhibited by S. jambos leaf extract, although gram-positive bacteria appeared more susceptible. Two of the 10 gram-negative bacteria (20%) and 2 of the 4 gram-positive bacteria (50%) tested had their growths inhibited by the extract. The leaf extract also proved to be toxic in the Artemia franciscana bioassay, with a 48-h LC(50) of 387.9 ± 38.8 µg/mL, making it slightly more toxic than Mevinphos (505.3± 37.7 µg/mL) and approximately 5-fold less toxic than potassium dichromate (80.4 ± 4.3 µg/mL). Whilst potassium dichromate's LC(50) remained constant across the 72-hour test period (24-h LC(50), 86.3 ± 5.1; 72-h LC(50), 77.9 ± 4.9), the extract and Mevinphos LC(50) values decreased by 72 hours (87.0 ± 11.3 µg/mL and 103.9 ± 12.8 µg/mL, respectively), indicating their similar levels of toxicity in the assay.

No MeSH data available.


Related in: MedlinePlus

Dependence of A. franciscana mortality on S. jambos leaf extract concentration following exposure for (a) 24 h, (b) 48 h and (c) 72 h. All bioassays were performed in at least triplicate and are expressed as mean ± standard deviation
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Figure 2: Dependence of A. franciscana mortality on S. jambos leaf extract concentration following exposure for (a) 24 h, (b) 48 h and (c) 72 h. All bioassays were performed in at least triplicate and are expressed as mean ± standard deviation

Mentions: To determine the effect of toxin concentration on the induction of mortality, the extract was diluted in artificial seawater to test across the concentration range 1000 µg/ mL to 15µg/mL in the Artemia nauplii bioassay at 24, 48 and 72 hours [Figure 2]. Table 3 shows the LC50 values of S. jambos extracts towards A. franciscana. No 24- hour LC50 values are reported for the S. jambos extracts as less than 50% mortality was seen by this time for all concentrations tested. The S. jambos leaf extract was slightly more toxic than Mevinphos at 48 hours, with LC50 values of 387.9 ± 38.8 and 505.3 ± 37.7, respectively. Potassium dichromate was substantially more toxic (80.4 ± 4.3) at 48 hours. The extract and both reference toxins showed similar toxicity at 72 hours as seen by their LC50 values.


Bioactivity of Syzygium jambos methanolic extracts: Antibacterial activity and toxicity.

Mohanty S, Cock IE - Pharmacognosy Res (2010)

Dependence of A. franciscana mortality on S. jambos leaf extract concentration following exposure for (a) 24 h, (b) 48 h and (c) 72 h. All bioassays were performed in at least triplicate and are expressed as mean ± standard deviation
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3140127&req=5

Figure 2: Dependence of A. franciscana mortality on S. jambos leaf extract concentration following exposure for (a) 24 h, (b) 48 h and (c) 72 h. All bioassays were performed in at least triplicate and are expressed as mean ± standard deviation
Mentions: To determine the effect of toxin concentration on the induction of mortality, the extract was diluted in artificial seawater to test across the concentration range 1000 µg/ mL to 15µg/mL in the Artemia nauplii bioassay at 24, 48 and 72 hours [Figure 2]. Table 3 shows the LC50 values of S. jambos extracts towards A. franciscana. No 24- hour LC50 values are reported for the S. jambos extracts as less than 50% mortality was seen by this time for all concentrations tested. The S. jambos leaf extract was slightly more toxic than Mevinphos at 48 hours, with LC50 values of 387.9 ± 38.8 and 505.3 ± 37.7, respectively. Potassium dichromate was substantially more toxic (80.4 ± 4.3) at 48 hours. The extract and both reference toxins showed similar toxicity at 72 hours as seen by their LC50 values.

Bottom Line: Methanol extracts from S. jambos leaves were tested for antimicrobial activity and toxicity.The leaf extract also proved to be toxic in the Artemia franciscana bioassay, with a 48-h LC(50) of 387.9 ± 38.8 µg/mL, making it slightly more toxic than Mevinphos (505.3± 37.7 µg/mL) and approximately 5-fold less toxic than potassium dichromate (80.4 ± 4.3 µg/mL).Whilst potassium dichromate's LC(50) remained constant across the 72-hour test period (24-h LC(50), 86.3 ± 5.1; 72-h LC(50), 77.9 ± 4.9), the extract and Mevinphos LC(50) values decreased by 72 hours (87.0 ± 11.3 µg/mL and 103.9 ± 12.8 µg/mL, respectively), indicating their similar levels of toxicity in the assay.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomolecular and Physical Sciences, Nathan Campus, Griffith University, 170 Kessels Rd., Nathan, Brisbane, Queensland 4111, Australia.

ABSTRACT
Methanol extracts from S. jambos leaves were tested for antimicrobial activity and toxicity. S. jambos leaf extract inhibited the growth of 4 of the 14 bacteria tested (29%). Both gram-positive and gram-negative bacterial growths were inhibited by S. jambos leaf extract, although gram-positive bacteria appeared more susceptible. Two of the 10 gram-negative bacteria (20%) and 2 of the 4 gram-positive bacteria (50%) tested had their growths inhibited by the extract. The leaf extract also proved to be toxic in the Artemia franciscana bioassay, with a 48-h LC(50) of 387.9 ± 38.8 µg/mL, making it slightly more toxic than Mevinphos (505.3± 37.7 µg/mL) and approximately 5-fold less toxic than potassium dichromate (80.4 ± 4.3 µg/mL). Whilst potassium dichromate's LC(50) remained constant across the 72-hour test period (24-h LC(50), 86.3 ± 5.1; 72-h LC(50), 77.9 ± 4.9), the extract and Mevinphos LC(50) values decreased by 72 hours (87.0 ± 11.3 µg/mL and 103.9 ± 12.8 µg/mL, respectively), indicating their similar levels of toxicity in the assay.

No MeSH data available.


Related in: MedlinePlus