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Antiarthritic and antioxidant effects of the leaf extract of Ficus exasperata P. Beauv. (Moraceae).

Abotsi WM, Woode E, Ainooson GK, Amo-Barimah AK, Boakye-Gyasi E - Pharmacognosy Res (2010)

Bottom Line: The disease-modifying antirheumatic drug methotrexate (0.1-1 mg/kg i.p.) and the steroidal anti-inflammatory agent dexamethasone (0.3-3 mg/kg i.p.) also reduced very significantly the total polyarthritic edema as well as the spread of the arthritis from the ipsilateral to the contralateral paws of the treated animals.The extract also exhibited reducing activity (EC(50) = 8.105 ± 18.49), scavenged 2,2-diphenyl-1-picrylhydrazyl (DPPH, EC(50) = 0.499 ± 0.302) and prevented lipid peroxidation (IC(50) = 1.283 ± 0.923) in rat brain homogenates.These results suggest that ethanolic extract of the leaves of F. exasperata exerts antiarthritic activity after oral administration and also has antioxidant properties which may contribute to its activity.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, Faculty of Pharmacy and Pharmaceutical Sciences, College of Health Sciences, Kwame Nkrumah University of Science & Technology, Kumasi, Ghana.

ABSTRACT
Leaf extracts of Ficus exasperata P. Beauv. (Moraceae) are commonly used in Ghanaian traditional medicine for the treatment of several pathological states including inflammatory disorders. The present study was undertaken to evaluate the antiarthritic effect of an ethanolic extract of F. exasperata (FEE) in the Freund's adjuvant-induced arthritis model in rats. Since free radicals and reactive oxygen species are implicated in inflammatory joint diseases such as rheumatoid arthritis, the antioxidant potential of the extract was investigated in in vitro experimental models. FEE as well as the positive controls, dexamethasone and methotrexate, showed significant dose-dependent antiarthritic properties when applied to established adjuvant arthritis. Oral administration of FEE (30-300 mg/kg p.o.) significantly reduced the arthritic edema in the ipsilateral paw of rats with a maximal inhibition of 34.46 ± 11.42%. FEE (30-300 mg/kg p.o.) also significantly prevented the spread of the edema from the ipsilateral to the contralateral paws indicating inhibition of systemic spread. The disease-modifying antirheumatic drug methotrexate (0.1-1 mg/kg i.p.) and the steroidal anti-inflammatory agent dexamethasone (0.3-3 mg/kg i.p.) also reduced very significantly the total polyarthritic edema as well as the spread of the arthritis from the ipsilateral to the contralateral paws of the treated animals. The extract also exhibited reducing activity (EC(50) = 8.105 ± 18.49), scavenged 2,2-diphenyl-1-picrylhydrazyl (DPPH, EC(50) = 0.499 ± 0.302) and prevented lipid peroxidation (IC(50) = 1.283 ± 0.923) in rat brain homogenates. Phenols were detected in the extract. These results suggest that ethanolic extract of the leaves of F. exasperata exerts antiarthritic activity after oral administration and also has antioxidant properties which may contribute to its activity.

No MeSH data available.


Related in: MedlinePlus

Dose–response curves for FEE (30–300 mg/kg p.o.), methotrexate (0.1–1 mg/kg i.p.) and dexamethasone (0.3–3.0 mg/kg i.p.) on AIA in rats
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Figure 2: Dose–response curves for FEE (30–300 mg/kg p.o.), methotrexate (0.1–1 mg/kg i.p.) and dexamethasone (0.3–3.0 mg/kg i.p.) on AIA in rats

Mentions: Dose–response curves for the inhibition of foot edema are shown in Fig. 2. FEE (ED50 531.3 ± 237.83 mg/kg) was the least potent compared to methotrexate (ED50 0.41 ± 0.14 mg/kg; F1,28 = 52.45, P < 0.0001) and dexamethasone (ED50 0.11 ±0.02 mg/kg; F1,25 = 337.1, P < 0.0001) while dexamethasone was the most potent [Figure 2 and Table 1].


Antiarthritic and antioxidant effects of the leaf extract of Ficus exasperata P. Beauv. (Moraceae).

Abotsi WM, Woode E, Ainooson GK, Amo-Barimah AK, Boakye-Gyasi E - Pharmacognosy Res (2010)

Dose–response curves for FEE (30–300 mg/kg p.o.), methotrexate (0.1–1 mg/kg i.p.) and dexamethasone (0.3–3.0 mg/kg i.p.) on AIA in rats
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3140113&req=5

Figure 2: Dose–response curves for FEE (30–300 mg/kg p.o.), methotrexate (0.1–1 mg/kg i.p.) and dexamethasone (0.3–3.0 mg/kg i.p.) on AIA in rats
Mentions: Dose–response curves for the inhibition of foot edema are shown in Fig. 2. FEE (ED50 531.3 ± 237.83 mg/kg) was the least potent compared to methotrexate (ED50 0.41 ± 0.14 mg/kg; F1,28 = 52.45, P < 0.0001) and dexamethasone (ED50 0.11 ±0.02 mg/kg; F1,25 = 337.1, P < 0.0001) while dexamethasone was the most potent [Figure 2 and Table 1].

Bottom Line: The disease-modifying antirheumatic drug methotrexate (0.1-1 mg/kg i.p.) and the steroidal anti-inflammatory agent dexamethasone (0.3-3 mg/kg i.p.) also reduced very significantly the total polyarthritic edema as well as the spread of the arthritis from the ipsilateral to the contralateral paws of the treated animals.The extract also exhibited reducing activity (EC(50) = 8.105 ± 18.49), scavenged 2,2-diphenyl-1-picrylhydrazyl (DPPH, EC(50) = 0.499 ± 0.302) and prevented lipid peroxidation (IC(50) = 1.283 ± 0.923) in rat brain homogenates.These results suggest that ethanolic extract of the leaves of F. exasperata exerts antiarthritic activity after oral administration and also has antioxidant properties which may contribute to its activity.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, Faculty of Pharmacy and Pharmaceutical Sciences, College of Health Sciences, Kwame Nkrumah University of Science & Technology, Kumasi, Ghana.

ABSTRACT
Leaf extracts of Ficus exasperata P. Beauv. (Moraceae) are commonly used in Ghanaian traditional medicine for the treatment of several pathological states including inflammatory disorders. The present study was undertaken to evaluate the antiarthritic effect of an ethanolic extract of F. exasperata (FEE) in the Freund's adjuvant-induced arthritis model in rats. Since free radicals and reactive oxygen species are implicated in inflammatory joint diseases such as rheumatoid arthritis, the antioxidant potential of the extract was investigated in in vitro experimental models. FEE as well as the positive controls, dexamethasone and methotrexate, showed significant dose-dependent antiarthritic properties when applied to established adjuvant arthritis. Oral administration of FEE (30-300 mg/kg p.o.) significantly reduced the arthritic edema in the ipsilateral paw of rats with a maximal inhibition of 34.46 ± 11.42%. FEE (30-300 mg/kg p.o.) also significantly prevented the spread of the edema from the ipsilateral to the contralateral paws indicating inhibition of systemic spread. The disease-modifying antirheumatic drug methotrexate (0.1-1 mg/kg i.p.) and the steroidal anti-inflammatory agent dexamethasone (0.3-3 mg/kg i.p.) also reduced very significantly the total polyarthritic edema as well as the spread of the arthritis from the ipsilateral to the contralateral paws of the treated animals. The extract also exhibited reducing activity (EC(50) = 8.105 ± 18.49), scavenged 2,2-diphenyl-1-picrylhydrazyl (DPPH, EC(50) = 0.499 ± 0.302) and prevented lipid peroxidation (IC(50) = 1.283 ± 0.923) in rat brain homogenates. Phenols were detected in the extract. These results suggest that ethanolic extract of the leaves of F. exasperata exerts antiarthritic activity after oral administration and also has antioxidant properties which may contribute to its activity.

No MeSH data available.


Related in: MedlinePlus