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Antiarthritic and antioxidant effects of the leaf extract of Ficus exasperata P. Beauv. (Moraceae).

Abotsi WM, Woode E, Ainooson GK, Amo-Barimah AK, Boakye-Gyasi E - Pharmacognosy Res (2010)

Bottom Line: The disease-modifying antirheumatic drug methotrexate (0.1-1 mg/kg i.p.) and the steroidal anti-inflammatory agent dexamethasone (0.3-3 mg/kg i.p.) also reduced very significantly the total polyarthritic edema as well as the spread of the arthritis from the ipsilateral to the contralateral paws of the treated animals.The extract also exhibited reducing activity (EC(50) = 8.105 ± 18.49), scavenged 2,2-diphenyl-1-picrylhydrazyl (DPPH, EC(50) = 0.499 ± 0.302) and prevented lipid peroxidation (IC(50) = 1.283 ± 0.923) in rat brain homogenates.These results suggest that ethanolic extract of the leaves of F. exasperata exerts antiarthritic activity after oral administration and also has antioxidant properties which may contribute to its activity.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, Faculty of Pharmacy and Pharmaceutical Sciences, College of Health Sciences, Kwame Nkrumah University of Science & Technology, Kumasi, Ghana.

ABSTRACT
Leaf extracts of Ficus exasperata P. Beauv. (Moraceae) are commonly used in Ghanaian traditional medicine for the treatment of several pathological states including inflammatory disorders. The present study was undertaken to evaluate the antiarthritic effect of an ethanolic extract of F. exasperata (FEE) in the Freund's adjuvant-induced arthritis model in rats. Since free radicals and reactive oxygen species are implicated in inflammatory joint diseases such as rheumatoid arthritis, the antioxidant potential of the extract was investigated in in vitro experimental models. FEE as well as the positive controls, dexamethasone and methotrexate, showed significant dose-dependent antiarthritic properties when applied to established adjuvant arthritis. Oral administration of FEE (30-300 mg/kg p.o.) significantly reduced the arthritic edema in the ipsilateral paw of rats with a maximal inhibition of 34.46 ± 11.42%. FEE (30-300 mg/kg p.o.) also significantly prevented the spread of the edema from the ipsilateral to the contralateral paws indicating inhibition of systemic spread. The disease-modifying antirheumatic drug methotrexate (0.1-1 mg/kg i.p.) and the steroidal anti-inflammatory agent dexamethasone (0.3-3 mg/kg i.p.) also reduced very significantly the total polyarthritic edema as well as the spread of the arthritis from the ipsilateral to the contralateral paws of the treated animals. The extract also exhibited reducing activity (EC(50) = 8.105 ± 18.49), scavenged 2,2-diphenyl-1-picrylhydrazyl (DPPH, EC(50) = 0.499 ± 0.302) and prevented lipid peroxidation (IC(50) = 1.283 ± 0.923) in rat brain homogenates. Phenols were detected in the extract. These results suggest that ethanolic extract of the leaves of F. exasperata exerts antiarthritic activity after oral administration and also has antioxidant properties which may contribute to its activity.

No MeSH data available.


Related in: MedlinePlus

Effect of FEE (30–300 mg/kg p.o.), dexamethasone (0.3–3 mg/kg i.p.) and methotrexate (0.1–1 mg/kg i.p.) on time–course curve (a, c and e, respectively) and the total edema response (b, d and f, respectively) in AIA in rats. The total edema was calculated as AUCs over the 19-day period of drug treatment. Values are means ± SEM (n = 5). ***P < 0.001; **P < 0.01; *P < 0.05 compared to vehicle-treated group (two-way ANOVA followed by Bonferroni's post hoc test). †††P < 0.001; ††P < 0.01; †P < 0.05 compared to vehicle-treated group (oneway ANOVA followed by Newman–Keul's post hoc test)
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Figure 1: Effect of FEE (30–300 mg/kg p.o.), dexamethasone (0.3–3 mg/kg i.p.) and methotrexate (0.1–1 mg/kg i.p.) on time–course curve (a, c and e, respectively) and the total edema response (b, d and f, respectively) in AIA in rats. The total edema was calculated as AUCs over the 19-day period of drug treatment. Values are means ± SEM (n = 5). ***P < 0.001; **P < 0.01; *P < 0.05 compared to vehicle-treated group (two-way ANOVA followed by Bonferroni's post hoc test). †††P < 0.001; ††P < 0.01; †P < 0.05 compared to vehicle-treated group (oneway ANOVA followed by Newman–Keul's post hoc test)

Mentions: FEE (30–300 mg/kg p.o.) dose-dependently and significantly reduced the total ipslateral paw edema response over the 19 days of treatment with a maximal inhibition of 34.46 ± 11.42% [Figure 1b]. Similarly, the DMARD methotrexate (0.1–1 mg/kg i.p.) and the steroidal anti-inflammatory agent dexamethasone (0.3–3 mg/kg i.p.) profoundly reduced the total ipslateral paw edema by 53.09 ± 8.87% [Figure 1f] and 91.59 ± 2.06% [Figure 1d], respectively. FEE (30–300 mg/kg) also significantly (F3,144 = 5.38, P = 0.0094) reduced the extent of spread of edema form the ipsilateral to the contralateral paws indicating inhibition of systemic spread [Figure 1a, b]. Dexamethasone (F3,144 = 32.23, P < 0.0001) and methotrexate (F3,16 = 19.54, P < 0.0001) also completely prevented the spread of the arthritis from the ipsilateral to the contralateral paws of the treated animals [Figure 1d, f].


Antiarthritic and antioxidant effects of the leaf extract of Ficus exasperata P. Beauv. (Moraceae).

Abotsi WM, Woode E, Ainooson GK, Amo-Barimah AK, Boakye-Gyasi E - Pharmacognosy Res (2010)

Effect of FEE (30–300 mg/kg p.o.), dexamethasone (0.3–3 mg/kg i.p.) and methotrexate (0.1–1 mg/kg i.p.) on time–course curve (a, c and e, respectively) and the total edema response (b, d and f, respectively) in AIA in rats. The total edema was calculated as AUCs over the 19-day period of drug treatment. Values are means ± SEM (n = 5). ***P < 0.001; **P < 0.01; *P < 0.05 compared to vehicle-treated group (two-way ANOVA followed by Bonferroni's post hoc test). †††P < 0.001; ††P < 0.01; †P < 0.05 compared to vehicle-treated group (oneway ANOVA followed by Newman–Keul's post hoc test)
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3140113&req=5

Figure 1: Effect of FEE (30–300 mg/kg p.o.), dexamethasone (0.3–3 mg/kg i.p.) and methotrexate (0.1–1 mg/kg i.p.) on time–course curve (a, c and e, respectively) and the total edema response (b, d and f, respectively) in AIA in rats. The total edema was calculated as AUCs over the 19-day period of drug treatment. Values are means ± SEM (n = 5). ***P < 0.001; **P < 0.01; *P < 0.05 compared to vehicle-treated group (two-way ANOVA followed by Bonferroni's post hoc test). †††P < 0.001; ††P < 0.01; †P < 0.05 compared to vehicle-treated group (oneway ANOVA followed by Newman–Keul's post hoc test)
Mentions: FEE (30–300 mg/kg p.o.) dose-dependently and significantly reduced the total ipslateral paw edema response over the 19 days of treatment with a maximal inhibition of 34.46 ± 11.42% [Figure 1b]. Similarly, the DMARD methotrexate (0.1–1 mg/kg i.p.) and the steroidal anti-inflammatory agent dexamethasone (0.3–3 mg/kg i.p.) profoundly reduced the total ipslateral paw edema by 53.09 ± 8.87% [Figure 1f] and 91.59 ± 2.06% [Figure 1d], respectively. FEE (30–300 mg/kg) also significantly (F3,144 = 5.38, P = 0.0094) reduced the extent of spread of edema form the ipsilateral to the contralateral paws indicating inhibition of systemic spread [Figure 1a, b]. Dexamethasone (F3,144 = 32.23, P < 0.0001) and methotrexate (F3,16 = 19.54, P < 0.0001) also completely prevented the spread of the arthritis from the ipsilateral to the contralateral paws of the treated animals [Figure 1d, f].

Bottom Line: The disease-modifying antirheumatic drug methotrexate (0.1-1 mg/kg i.p.) and the steroidal anti-inflammatory agent dexamethasone (0.3-3 mg/kg i.p.) also reduced very significantly the total polyarthritic edema as well as the spread of the arthritis from the ipsilateral to the contralateral paws of the treated animals.The extract also exhibited reducing activity (EC(50) = 8.105 ± 18.49), scavenged 2,2-diphenyl-1-picrylhydrazyl (DPPH, EC(50) = 0.499 ± 0.302) and prevented lipid peroxidation (IC(50) = 1.283 ± 0.923) in rat brain homogenates.These results suggest that ethanolic extract of the leaves of F. exasperata exerts antiarthritic activity after oral administration and also has antioxidant properties which may contribute to its activity.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, Faculty of Pharmacy and Pharmaceutical Sciences, College of Health Sciences, Kwame Nkrumah University of Science & Technology, Kumasi, Ghana.

ABSTRACT
Leaf extracts of Ficus exasperata P. Beauv. (Moraceae) are commonly used in Ghanaian traditional medicine for the treatment of several pathological states including inflammatory disorders. The present study was undertaken to evaluate the antiarthritic effect of an ethanolic extract of F. exasperata (FEE) in the Freund's adjuvant-induced arthritis model in rats. Since free radicals and reactive oxygen species are implicated in inflammatory joint diseases such as rheumatoid arthritis, the antioxidant potential of the extract was investigated in in vitro experimental models. FEE as well as the positive controls, dexamethasone and methotrexate, showed significant dose-dependent antiarthritic properties when applied to established adjuvant arthritis. Oral administration of FEE (30-300 mg/kg p.o.) significantly reduced the arthritic edema in the ipsilateral paw of rats with a maximal inhibition of 34.46 ± 11.42%. FEE (30-300 mg/kg p.o.) also significantly prevented the spread of the edema from the ipsilateral to the contralateral paws indicating inhibition of systemic spread. The disease-modifying antirheumatic drug methotrexate (0.1-1 mg/kg i.p.) and the steroidal anti-inflammatory agent dexamethasone (0.3-3 mg/kg i.p.) also reduced very significantly the total polyarthritic edema as well as the spread of the arthritis from the ipsilateral to the contralateral paws of the treated animals. The extract also exhibited reducing activity (EC(50) = 8.105 ± 18.49), scavenged 2,2-diphenyl-1-picrylhydrazyl (DPPH, EC(50) = 0.499 ± 0.302) and prevented lipid peroxidation (IC(50) = 1.283 ± 0.923) in rat brain homogenates. Phenols were detected in the extract. These results suggest that ethanolic extract of the leaves of F. exasperata exerts antiarthritic activity after oral administration and also has antioxidant properties which may contribute to its activity.

No MeSH data available.


Related in: MedlinePlus