Limits...
Analgesic activity, toxicity study and phytochemical screening of standardized Cinnomomum iners leaves methanolic extract.

Mustaffa F, Indurkar J, Ismail S, Mordi MN, Ramanathan S, Mansor SM - Pharmacognosy Res (2010)

Bottom Line: However, CSLE did not show activity in the hot plate and tail flick tests.The results obtained suggest that CSLE acts peripherally to relieve pain.Phytochemical screening of CSLE showed the presence of cardiac glycoside, flavonoid, polyphenol, saponin, sugar, tannin and terpenoid.

View Article: PubMed Central - PubMed

Affiliation: Centre for Drug Research, Universiti Sains Malaysia, 11800, Penang, Malaysia.

ABSTRACT
Cinnomomum iners standardized leaves methanolic extract (CSLE) was subjected to analgesic, toxicity and phytochemical studies. The analgesic activity of CSLE was evaluated using formalin, hot plate and tail flick tests at doses of 100, 200 and 500 mg/kg. CSLE showed significant activity (P < 0.05) in the formalin model (late phase) on the rats at doses of 200 and 500 mg/kg. However, CSLE did not show activity in the hot plate and tail flick tests. The results obtained suggest that CSLE acts peripherally to relieve pain. For the toxicity study, CSLE was orally administered to the Swiss albino mice according to the Organization for Economic Co-Operation and Development (OECD) guideline 423. There was no lethality or toxic symptoms observed for all the tested doses throughout the 14-day period. Phytochemical screening of CSLE showed the presence of cardiac glycoside, flavonoid, polyphenol, saponin, sugar, tannin and terpenoid.

No MeSH data available.


Related in: MedlinePlus

An overlay GC–MS chromatogram of C. iners extract and standard beta-caryophyllene
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC3140111&req=5

Figure 1: An overlay GC–MS chromatogram of C. iners extract and standard beta-caryophyllene

Mentions: Beta-caryophyllene in C. iners leaves extract chromatogram was identified by matching of retention time and mass spectra provided by MS library and purchased marker standard (beta-caryophyllene) [Figures 1 and 2]. Beta-caryophyllene gives well-distinguished peaks at retention time of 6.078 minutes as depicted in Figure 1. As for the standardization, the quantitation of beta-caryophyllene was based on the peak area calculated from the calibration curve equation (y = 11544x – 15831, r2 = 0.997). The amount of beta-caryophyllene present was found to be 0.048 mg (4.8%) in 1 mg of C. iners leaves methanolic extract.


Analgesic activity, toxicity study and phytochemical screening of standardized Cinnomomum iners leaves methanolic extract.

Mustaffa F, Indurkar J, Ismail S, Mordi MN, Ramanathan S, Mansor SM - Pharmacognosy Res (2010)

An overlay GC–MS chromatogram of C. iners extract and standard beta-caryophyllene
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3140111&req=5

Figure 1: An overlay GC–MS chromatogram of C. iners extract and standard beta-caryophyllene
Mentions: Beta-caryophyllene in C. iners leaves extract chromatogram was identified by matching of retention time and mass spectra provided by MS library and purchased marker standard (beta-caryophyllene) [Figures 1 and 2]. Beta-caryophyllene gives well-distinguished peaks at retention time of 6.078 minutes as depicted in Figure 1. As for the standardization, the quantitation of beta-caryophyllene was based on the peak area calculated from the calibration curve equation (y = 11544x – 15831, r2 = 0.997). The amount of beta-caryophyllene present was found to be 0.048 mg (4.8%) in 1 mg of C. iners leaves methanolic extract.

Bottom Line: However, CSLE did not show activity in the hot plate and tail flick tests.The results obtained suggest that CSLE acts peripherally to relieve pain.Phytochemical screening of CSLE showed the presence of cardiac glycoside, flavonoid, polyphenol, saponin, sugar, tannin and terpenoid.

View Article: PubMed Central - PubMed

Affiliation: Centre for Drug Research, Universiti Sains Malaysia, 11800, Penang, Malaysia.

ABSTRACT
Cinnomomum iners standardized leaves methanolic extract (CSLE) was subjected to analgesic, toxicity and phytochemical studies. The analgesic activity of CSLE was evaluated using formalin, hot plate and tail flick tests at doses of 100, 200 and 500 mg/kg. CSLE showed significant activity (P < 0.05) in the formalin model (late phase) on the rats at doses of 200 and 500 mg/kg. However, CSLE did not show activity in the hot plate and tail flick tests. The results obtained suggest that CSLE acts peripherally to relieve pain. For the toxicity study, CSLE was orally administered to the Swiss albino mice according to the Organization for Economic Co-Operation and Development (OECD) guideline 423. There was no lethality or toxic symptoms observed for all the tested doses throughout the 14-day period. Phytochemical screening of CSLE showed the presence of cardiac glycoside, flavonoid, polyphenol, saponin, sugar, tannin and terpenoid.

No MeSH data available.


Related in: MedlinePlus