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Wound healing properties and kill kinetics of Clerodendron splendens G. Don, a Ghanaian wound healing plant.

Gbedema SY, Emelia K, Francis A, Kofi A, Eric W - Pharmacognosy Res (2010)

Bottom Line: The extract exhibited biostatic action against all the test microorganisms with a Minimum Inhibition Concentration (MIC) ranging between 64 and 512 μg/ml and a free radical scavenging property with an IC(50) value of 103.2 μg/ml.Significant increase in the tensile strength and hydroxyproline content were also observed as compared to the control and was comparable to nitrofurazone.The above results appear to justify the traditional use of C. splendens in wound healing and treatment of skin infections in Ghana.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmaceutics, Faculty of Pharmacy and Pharmaceutical Sciences, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana, West Africa.

ABSTRACT
As part of our general objective of investigating indigenous plants used in wound healing in Ghana, we hereby report our findings from some in vitro and in vivo studies related to wound healing activities of Clerodendron splendens G. Don (Verbanaceae). Methanolic extract of the aerial parts of the plant was tested for antimicrobial activity against Gram positive bacteria (Bacillus subtilis, Staphylococcus aureus, Streptococcus faecalis, Micrococcus flavus, as well as resistant strains of Staph. aureus SA1199B, RN4220 and XU212), Gram negative bacteria (Escherichia coli, Pseudomonas aeruginosa, Proteous mirabilis, Klebsiella pneumoniae) and Candida albicans using the micro-well dilution method. Survivor-time studies of the microorganisms, radical scavenging activity using 2,2'-diphenylpicrylhydrazyl (DPPH) and various in vivo wound healing activity studies were also conducted on the extract. The extract exhibited biostatic action against all the test microorganisms with a Minimum Inhibition Concentration (MIC) ranging between 64 and 512 μg/ml and a free radical scavenging property with an IC(50) value of 103.2 μg/ml. The results of the in vivo wound healing tests showed that upon application of C. splendens ointment, there was a reduction in the epithelization period from 26.7 days (control) to 13.6 days along with a marked decrease in the scar area from 54.2 mm(2) (control) to 25.2 mm(2). Significant increase in the tensile strength and hydroxyproline content were also observed as compared to the control and was comparable to nitrofurazone. The above results appear to justify the traditional use of C. splendens in wound healing and treatment of skin infections in Ghana.

No MeSH data available.


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Survival of (a) Staph. aureus (b) P. aeruginosa and (c) C. albicans in the presence of various concentrations of C. splendens extract
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Figure 1: Survival of (a) Staph. aureus (b) P. aeruginosa and (c) C. albicans in the presence of various concentrations of C. splendens extract

Mentions: The MIC of the extract against the resistant organisms were however, higher. The controls used (tetracycline and neomycin) recorded very low MICs thus confirming their superiority over the plant extract as an antimicrobial agent. The means of survivors obtained at each concentration plated for each organism are graphically presented and samples are as shown in Figure 1 for S. aureus, P. aeruginosa and C. albicans. The graphs of the other test organisms (not shown) followed similar partten. All the organisms exhibited a decline in survivors in the first 4–6 h of exposure to the extract in concentration-dependent manner whilst the controls showed a steady rise in population within these same periods, followed by some level of recovery of the organisms even at higher concentrations. This depicts biostatic mode of action of the extract. The recoveries may be due to inactivation of the bioactive constituents of the extract with time when in the nutrient medium. This might have necessitated the daily collection of this herb for treatment by the local herbal practitioners.


Wound healing properties and kill kinetics of Clerodendron splendens G. Don, a Ghanaian wound healing plant.

Gbedema SY, Emelia K, Francis A, Kofi A, Eric W - Pharmacognosy Res (2010)

Survival of (a) Staph. aureus (b) P. aeruginosa and (c) C. albicans in the presence of various concentrations of C. splendens extract
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3140108&req=5

Figure 1: Survival of (a) Staph. aureus (b) P. aeruginosa and (c) C. albicans in the presence of various concentrations of C. splendens extract
Mentions: The MIC of the extract against the resistant organisms were however, higher. The controls used (tetracycline and neomycin) recorded very low MICs thus confirming their superiority over the plant extract as an antimicrobial agent. The means of survivors obtained at each concentration plated for each organism are graphically presented and samples are as shown in Figure 1 for S. aureus, P. aeruginosa and C. albicans. The graphs of the other test organisms (not shown) followed similar partten. All the organisms exhibited a decline in survivors in the first 4–6 h of exposure to the extract in concentration-dependent manner whilst the controls showed a steady rise in population within these same periods, followed by some level of recovery of the organisms even at higher concentrations. This depicts biostatic mode of action of the extract. The recoveries may be due to inactivation of the bioactive constituents of the extract with time when in the nutrient medium. This might have necessitated the daily collection of this herb for treatment by the local herbal practitioners.

Bottom Line: The extract exhibited biostatic action against all the test microorganisms with a Minimum Inhibition Concentration (MIC) ranging between 64 and 512 μg/ml and a free radical scavenging property with an IC(50) value of 103.2 μg/ml.Significant increase in the tensile strength and hydroxyproline content were also observed as compared to the control and was comparable to nitrofurazone.The above results appear to justify the traditional use of C. splendens in wound healing and treatment of skin infections in Ghana.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmaceutics, Faculty of Pharmacy and Pharmaceutical Sciences, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana, West Africa.

ABSTRACT
As part of our general objective of investigating indigenous plants used in wound healing in Ghana, we hereby report our findings from some in vitro and in vivo studies related to wound healing activities of Clerodendron splendens G. Don (Verbanaceae). Methanolic extract of the aerial parts of the plant was tested for antimicrobial activity against Gram positive bacteria (Bacillus subtilis, Staphylococcus aureus, Streptococcus faecalis, Micrococcus flavus, as well as resistant strains of Staph. aureus SA1199B, RN4220 and XU212), Gram negative bacteria (Escherichia coli, Pseudomonas aeruginosa, Proteous mirabilis, Klebsiella pneumoniae) and Candida albicans using the micro-well dilution method. Survivor-time studies of the microorganisms, radical scavenging activity using 2,2'-diphenylpicrylhydrazyl (DPPH) and various in vivo wound healing activity studies were also conducted on the extract. The extract exhibited biostatic action against all the test microorganisms with a Minimum Inhibition Concentration (MIC) ranging between 64 and 512 μg/ml and a free radical scavenging property with an IC(50) value of 103.2 μg/ml. The results of the in vivo wound healing tests showed that upon application of C. splendens ointment, there was a reduction in the epithelization period from 26.7 days (control) to 13.6 days along with a marked decrease in the scar area from 54.2 mm(2) (control) to 25.2 mm(2). Significant increase in the tensile strength and hydroxyproline content were also observed as compared to the control and was comparable to nitrofurazone. The above results appear to justify the traditional use of C. splendens in wound healing and treatment of skin infections in Ghana.

No MeSH data available.


Related in: MedlinePlus