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Bronchodilator activity of aqueous extract of stem bark of Ailanthus excelsa Roxb.

Kumar D, Bhujbal SS, Deoda RS, Mudgade SC - Pharmacognosy Res (2010)

Bottom Line: Stem bark of A. excelsa Roxb. has been used as a decoction in traditional claims.So, our traditional claims enforced us to evaluate its bronchodilator activity.The aqueous extract of stem bark in doses of 100, 200, 400 mg/kg showed significant activity.

View Article: PubMed Central - PubMed

Affiliation: Rayat Institute of Pharmacy, Railmajra, S.B.S. Nagar, Punjab, India.

ABSTRACT
Biologically active compounds from natural sources are of interest as possible new drugs for infectious diseases. Ailanthus excelsa Roxb. has been used in Indian system of medicine in the treatment of asthma, bronchitis, cold, colic pain, etc. Stem bark of A. excelsa Roxb. has been used as a decoction in traditional claims. So, our traditional claims enforced us to evaluate its bronchodilator activity. We have evaluated its bronchodilator activity in milk-induced leukocytosis and eosinophilia, clonidine-induced mast cell degranulation, bronchoalveolar lavage fluid (BALF), and lung histopathology models. The aqueous extract of stem bark in doses of 100, 200, 400 mg/kg showed significant activity.

No MeSH data available.


Related in: MedlinePlus

Effect of AESAq on histopathologic evaluation of lung tissue. Light micrograph of rat lungs collected from different treatment groups and the lungs were fixed in formalin and embedded in paraffin wax. Sections of lung tissue were cut at 5 μm thickness, mounted on glass slides and stained with hematoxylin and eosin (H × E) and cells were identified as either eosinophils, neutrophils or mononuclear cells by standard morphology and 200 cells counted under 400× magnification. a) NS - Absence of inflammatory cells, no edema in the lung tissue (25×). b) S- A low magnification lung section from an antigen-challenged animal showing evidence of Fluid accumulation along with inflammatory cells, blood cells, edema. c) A low magnification lung section from an antigenchallenged animal, received (Std.) Dexamethasone (1mg/kg, i.p.) showing absence of fluid accumulation around the blood vessel. d) A lung section from an antigen-challenged animal showing massive recruitment of eosinophils around the airway, blood vessels and bronchoconstuction, received AESAq100 (25×). e) A lung section from an antigen-challenged animal showing bronchodilation and blood vessels eosinophils around the airway, received AESAq200 (25×). f) A lung section from an antigenchallenged animal showing partial resolution of the tissue eosinophils around the airway and bronchodilation, received AESAq400
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Figure 4: Effect of AESAq on histopathologic evaluation of lung tissue. Light micrograph of rat lungs collected from different treatment groups and the lungs were fixed in formalin and embedded in paraffin wax. Sections of lung tissue were cut at 5 μm thickness, mounted on glass slides and stained with hematoxylin and eosin (H × E) and cells were identified as either eosinophils, neutrophils or mononuclear cells by standard morphology and 200 cells counted under 400× magnification. a) NS - Absence of inflammatory cells, no edema in the lung tissue (25×). b) S- A low magnification lung section from an antigen-challenged animal showing evidence of Fluid accumulation along with inflammatory cells, blood cells, edema. c) A low magnification lung section from an antigenchallenged animal, received (Std.) Dexamethasone (1mg/kg, i.p.) showing absence of fluid accumulation around the blood vessel. d) A lung section from an antigen-challenged animal showing massive recruitment of eosinophils around the airway, blood vessels and bronchoconstuction, received AESAq100 (25×). e) A lung section from an antigen-challenged animal showing bronchodilation and blood vessels eosinophils around the airway, received AESAq200 (25×). f) A lung section from an antigenchallenged animal showing partial resolution of the tissue eosinophils around the airway and bronchodilation, received AESAq400

Mentions: Histopathologic evaluation of lung tissue showed the significant bronchodilator at 400 mg/kg. (a) Absence of inflammatory cells, no edema in the lung tissue (NS). (b) Fluid accumulation along with inflammatory cells, blood cells, edema, (S). (c) Absence of fluid accumulation around the blood vessel, (Std.-dexamethasone). (d) Massive recruitment of eosinophils around the airway, blood vessels and bronchoconstuction (AESAq100). (e) Bronchodilation and blood vessels eosinophils around the airway (AESAq200). (f) Partial resolution of the tissue eosinophils around the airway and showing bronchodilator (AESAq400) [Figure 4].


Bronchodilator activity of aqueous extract of stem bark of Ailanthus excelsa Roxb.

Kumar D, Bhujbal SS, Deoda RS, Mudgade SC - Pharmacognosy Res (2010)

Effect of AESAq on histopathologic evaluation of lung tissue. Light micrograph of rat lungs collected from different treatment groups and the lungs were fixed in formalin and embedded in paraffin wax. Sections of lung tissue were cut at 5 μm thickness, mounted on glass slides and stained with hematoxylin and eosin (H × E) and cells were identified as either eosinophils, neutrophils or mononuclear cells by standard morphology and 200 cells counted under 400× magnification. a) NS - Absence of inflammatory cells, no edema in the lung tissue (25×). b) S- A low magnification lung section from an antigen-challenged animal showing evidence of Fluid accumulation along with inflammatory cells, blood cells, edema. c) A low magnification lung section from an antigenchallenged animal, received (Std.) Dexamethasone (1mg/kg, i.p.) showing absence of fluid accumulation around the blood vessel. d) A lung section from an antigen-challenged animal showing massive recruitment of eosinophils around the airway, blood vessels and bronchoconstuction, received AESAq100 (25×). e) A lung section from an antigen-challenged animal showing bronchodilation and blood vessels eosinophils around the airway, received AESAq200 (25×). f) A lung section from an antigenchallenged animal showing partial resolution of the tissue eosinophils around the airway and bronchodilation, received AESAq400
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3140105&req=5

Figure 4: Effect of AESAq on histopathologic evaluation of lung tissue. Light micrograph of rat lungs collected from different treatment groups and the lungs were fixed in formalin and embedded in paraffin wax. Sections of lung tissue were cut at 5 μm thickness, mounted on glass slides and stained with hematoxylin and eosin (H × E) and cells were identified as either eosinophils, neutrophils or mononuclear cells by standard morphology and 200 cells counted under 400× magnification. a) NS - Absence of inflammatory cells, no edema in the lung tissue (25×). b) S- A low magnification lung section from an antigen-challenged animal showing evidence of Fluid accumulation along with inflammatory cells, blood cells, edema. c) A low magnification lung section from an antigenchallenged animal, received (Std.) Dexamethasone (1mg/kg, i.p.) showing absence of fluid accumulation around the blood vessel. d) A lung section from an antigen-challenged animal showing massive recruitment of eosinophils around the airway, blood vessels and bronchoconstuction, received AESAq100 (25×). e) A lung section from an antigen-challenged animal showing bronchodilation and blood vessels eosinophils around the airway, received AESAq200 (25×). f) A lung section from an antigenchallenged animal showing partial resolution of the tissue eosinophils around the airway and bronchodilation, received AESAq400
Mentions: Histopathologic evaluation of lung tissue showed the significant bronchodilator at 400 mg/kg. (a) Absence of inflammatory cells, no edema in the lung tissue (NS). (b) Fluid accumulation along with inflammatory cells, blood cells, edema, (S). (c) Absence of fluid accumulation around the blood vessel, (Std.-dexamethasone). (d) Massive recruitment of eosinophils around the airway, blood vessels and bronchoconstuction (AESAq100). (e) Bronchodilation and blood vessels eosinophils around the airway (AESAq200). (f) Partial resolution of the tissue eosinophils around the airway and showing bronchodilator (AESAq400) [Figure 4].

Bottom Line: Stem bark of A. excelsa Roxb. has been used as a decoction in traditional claims.So, our traditional claims enforced us to evaluate its bronchodilator activity.The aqueous extract of stem bark in doses of 100, 200, 400 mg/kg showed significant activity.

View Article: PubMed Central - PubMed

Affiliation: Rayat Institute of Pharmacy, Railmajra, S.B.S. Nagar, Punjab, India.

ABSTRACT
Biologically active compounds from natural sources are of interest as possible new drugs for infectious diseases. Ailanthus excelsa Roxb. has been used in Indian system of medicine in the treatment of asthma, bronchitis, cold, colic pain, etc. Stem bark of A. excelsa Roxb. has been used as a decoction in traditional claims. So, our traditional claims enforced us to evaluate its bronchodilator activity. We have evaluated its bronchodilator activity in milk-induced leukocytosis and eosinophilia, clonidine-induced mast cell degranulation, bronchoalveolar lavage fluid (BALF), and lung histopathology models. The aqueous extract of stem bark in doses of 100, 200, 400 mg/kg showed significant activity.

No MeSH data available.


Related in: MedlinePlus