Limits...
Bronchodilator activity of aqueous extract of stem bark of Ailanthus excelsa Roxb.

Kumar D, Bhujbal SS, Deoda RS, Mudgade SC - Pharmacognosy Res (2010)

Bottom Line: Stem bark of A. excelsa Roxb. has been used as a decoction in traditional claims.So, our traditional claims enforced us to evaluate its bronchodilator activity.The aqueous extract of stem bark in doses of 100, 200, 400 mg/kg showed significant activity.

View Article: PubMed Central - PubMed

Affiliation: Rayat Institute of Pharmacy, Railmajra, S.B.S. Nagar, Punjab, India.

ABSTRACT
Biologically active compounds from natural sources are of interest as possible new drugs for infectious diseases. Ailanthus excelsa Roxb. has been used in Indian system of medicine in the treatment of asthma, bronchitis, cold, colic pain, etc. Stem bark of A. excelsa Roxb. has been used as a decoction in traditional claims. So, our traditional claims enforced us to evaluate its bronchodilator activity. We have evaluated its bronchodilator activity in milk-induced leukocytosis and eosinophilia, clonidine-induced mast cell degranulation, bronchoalveolar lavage fluid (BALF), and lung histopathology models. The aqueous extract of stem bark in doses of 100, 200, 400 mg/kg showed significant activity.

No MeSH data available.


Related in: MedlinePlus

Effect of AESAq on bronchoalveolar lavage in rats; n = 5, values are expressed in mean ± SEM. NS = nonsensitized group, distilled water + 8 mg alum in 1 ml (i.p.); S = sensitized group, ovalbumin 20 μg + 8 mg alum in 1 ml (i.p.) on days 1 and 7 and 1% OVA - aerosol on 15th day, dose was increased by 500 folds. ; Std. = dexamethasone (1 mg/kg, i.p.); AESAq 100 = A. excelsa Roxb. stem bark aqueous extract (100 mg/kg p.o.); AESAq 200 = A. excelsa Roxb. stem bark aqueous extract (200 mg/kg p.o); AESAq400 = A. excelsa Roxb. stem bark aqueous extract (400 mg/kg p.o.). NS compared with S by using student's-t test (***P < 0.001); Std., AESAq100, AESAq200, AESAq400 compared with S by ANOVA followed by Dunnett's test (*P < 0.05, **P < 0.01)
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC3140105&req=5

Figure 3: Effect of AESAq on bronchoalveolar lavage in rats; n = 5, values are expressed in mean ± SEM. NS = nonsensitized group, distilled water + 8 mg alum in 1 ml (i.p.); S = sensitized group, ovalbumin 20 μg + 8 mg alum in 1 ml (i.p.) on days 1 and 7 and 1% OVA - aerosol on 15th day, dose was increased by 500 folds. ; Std. = dexamethasone (1 mg/kg, i.p.); AESAq 100 = A. excelsa Roxb. stem bark aqueous extract (100 mg/kg p.o.); AESAq 200 = A. excelsa Roxb. stem bark aqueous extract (200 mg/kg p.o); AESAq400 = A. excelsa Roxb. stem bark aqueous extract (400 mg/kg p.o.). NS compared with S by using student's-t test (***P < 0.001); Std., AESAq100, AESAq200, AESAq400 compared with S by ANOVA followed by Dunnett's test (*P < 0.05, **P < 0.01)

Mentions: Persistent mucosal airway inflammation, associated with an increase in T helper type 2 (Th2) cytokine levels, eosinophil infiltration into the airways, and mucus and immunoglobulin (I) E production, are the main features of allergic asthma. The infiltration of cells like eosinophils, neutrophils, monocots, macrophages, lymphocytes, etc., increases the allergic asthmatic effect. Injection of ovalbumin 20 μg + 8 mg alum in 1 ml (i.p.) on days 1 and 7 and 1% OVA aerosol on 15th day produced a significant (***P < 0.001) increase in the TLC i.e. 4.41 fold than the nonsensitized group and differential leukocyte count. In the groups pretreated with standard drug dexamethasone (1 mg/kg i.p.), there was a significant (**P < 0.01) inhibition of ovalbumin-induced TLC and differential leukocyte count. The aqueous extract of A. excelsa Roxb. at doses of 100, 200, and 400mg/kg showed significant decrease in TLC and neutrophils, lymphocytes and monocots, (*P < 0.05, **P < 0.01) but in case of other differential leukocytes like macrophages and eosinophils, it showed an inhibition at 200 and 400 mg/kg p.o. (*P < 0.05, **P < 0.01) [Figure 3].


Bronchodilator activity of aqueous extract of stem bark of Ailanthus excelsa Roxb.

Kumar D, Bhujbal SS, Deoda RS, Mudgade SC - Pharmacognosy Res (2010)

Effect of AESAq on bronchoalveolar lavage in rats; n = 5, values are expressed in mean ± SEM. NS = nonsensitized group, distilled water + 8 mg alum in 1 ml (i.p.); S = sensitized group, ovalbumin 20 μg + 8 mg alum in 1 ml (i.p.) on days 1 and 7 and 1% OVA - aerosol on 15th day, dose was increased by 500 folds. ; Std. = dexamethasone (1 mg/kg, i.p.); AESAq 100 = A. excelsa Roxb. stem bark aqueous extract (100 mg/kg p.o.); AESAq 200 = A. excelsa Roxb. stem bark aqueous extract (200 mg/kg p.o); AESAq400 = A. excelsa Roxb. stem bark aqueous extract (400 mg/kg p.o.). NS compared with S by using student's-t test (***P < 0.001); Std., AESAq100, AESAq200, AESAq400 compared with S by ANOVA followed by Dunnett's test (*P < 0.05, **P < 0.01)
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3140105&req=5

Figure 3: Effect of AESAq on bronchoalveolar lavage in rats; n = 5, values are expressed in mean ± SEM. NS = nonsensitized group, distilled water + 8 mg alum in 1 ml (i.p.); S = sensitized group, ovalbumin 20 μg + 8 mg alum in 1 ml (i.p.) on days 1 and 7 and 1% OVA - aerosol on 15th day, dose was increased by 500 folds. ; Std. = dexamethasone (1 mg/kg, i.p.); AESAq 100 = A. excelsa Roxb. stem bark aqueous extract (100 mg/kg p.o.); AESAq 200 = A. excelsa Roxb. stem bark aqueous extract (200 mg/kg p.o); AESAq400 = A. excelsa Roxb. stem bark aqueous extract (400 mg/kg p.o.). NS compared with S by using student's-t test (***P < 0.001); Std., AESAq100, AESAq200, AESAq400 compared with S by ANOVA followed by Dunnett's test (*P < 0.05, **P < 0.01)
Mentions: Persistent mucosal airway inflammation, associated with an increase in T helper type 2 (Th2) cytokine levels, eosinophil infiltration into the airways, and mucus and immunoglobulin (I) E production, are the main features of allergic asthma. The infiltration of cells like eosinophils, neutrophils, monocots, macrophages, lymphocytes, etc., increases the allergic asthmatic effect. Injection of ovalbumin 20 μg + 8 mg alum in 1 ml (i.p.) on days 1 and 7 and 1% OVA aerosol on 15th day produced a significant (***P < 0.001) increase in the TLC i.e. 4.41 fold than the nonsensitized group and differential leukocyte count. In the groups pretreated with standard drug dexamethasone (1 mg/kg i.p.), there was a significant (**P < 0.01) inhibition of ovalbumin-induced TLC and differential leukocyte count. The aqueous extract of A. excelsa Roxb. at doses of 100, 200, and 400mg/kg showed significant decrease in TLC and neutrophils, lymphocytes and monocots, (*P < 0.05, **P < 0.01) but in case of other differential leukocytes like macrophages and eosinophils, it showed an inhibition at 200 and 400 mg/kg p.o. (*P < 0.05, **P < 0.01) [Figure 3].

Bottom Line: Stem bark of A. excelsa Roxb. has been used as a decoction in traditional claims.So, our traditional claims enforced us to evaluate its bronchodilator activity.The aqueous extract of stem bark in doses of 100, 200, 400 mg/kg showed significant activity.

View Article: PubMed Central - PubMed

Affiliation: Rayat Institute of Pharmacy, Railmajra, S.B.S. Nagar, Punjab, India.

ABSTRACT
Biologically active compounds from natural sources are of interest as possible new drugs for infectious diseases. Ailanthus excelsa Roxb. has been used in Indian system of medicine in the treatment of asthma, bronchitis, cold, colic pain, etc. Stem bark of A. excelsa Roxb. has been used as a decoction in traditional claims. So, our traditional claims enforced us to evaluate its bronchodilator activity. We have evaluated its bronchodilator activity in milk-induced leukocytosis and eosinophilia, clonidine-induced mast cell degranulation, bronchoalveolar lavage fluid (BALF), and lung histopathology models. The aqueous extract of stem bark in doses of 100, 200, 400 mg/kg showed significant activity.

No MeSH data available.


Related in: MedlinePlus