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Expression patterns of cancer-testis antigens in human embryonic stem cells and their cell derivatives indicate lineage tracks.

Lifantseva N, Koltsova A, Krylova T, Yakovleva T, Poljanskaya G, Gordeeva O - Stem Cells Int (2011)

Bottom Line: Undifferentiated hES cells and embryoid body cells expressed MAGE-A3, -A6, -A4, -A8, and GAGEs while later differentiated derivatives expressed only MAGE-A8 or MAGE-A4.Despite similarity of the hES and hEC cell expression patterns, MAGE-A2 and MAGE-B2 were detected only in hEC cells but not in hES cells.The identification of CTA expression patterns in pluripotent stem cells and their derivatives may be useful for isolation of abnormally CTA-expressing cells to improve the safety of stem-cell based therapy.

View Article: PubMed Central - PubMed

Affiliation: Kol'tsov Institute of Developmental Biology, Russian Academy of Sciences, 26 Vavilov Street, Moscow 119334, Russia.

ABSTRACT
Pluripotent stem cells can differentiate into various lineages but undergo genetic and epigenetic changes during long-term cultivation and, therefore, require regular monitoring. The expression patterns of cancer-testis antigens (CTAs) MAGE-A2, -A3, -A4, -A6, -A8, -B2, and GAGE were examined in undifferentiated human embryonic stem (hES) cells, their differentiated derivatives, teratocarcinoma (hEC) cells, and cancer cell lines of neuroectodermal and mesodermal origin. Undifferentiated hES cells and embryoid body cells expressed MAGE-A3, -A6, -A4, -A8, and GAGEs while later differentiated derivatives expressed only MAGE-A8 or MAGE-A4. Likewise, mouse pluripotent stem cells also express CTAs of Magea but not Mageb family. Despite similarity of the hES and hEC cell expression patterns, MAGE-A2 and MAGE-B2 were detected only in hEC cells but not in hES cells. Moreover, our analysis has shown that CTAs are aberrantly expressed in cancer cell lines and display low tissue specificity. The identification of CTA expression patterns in pluripotent stem cells and their derivatives may be useful for isolation of abnormally CTA-expressing cells to improve the safety of stem-cell based therapy.

No MeSH data available.


Related in: MedlinePlus

CTA expression in adult human and mouse testicular and brain samples. Designations: T: human testes, B: human brain.
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Related In: Results  -  Collection


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fig1: CTA expression in adult human and mouse testicular and brain samples. Designations: T: human testes, B: human brain.

Mentions: At first, we tested the primers for the detection of CTA expression by RT-PCR in normal human and mouse tissues used as positive and negative controls. The expression of MAGE-A2, -A3, -A6, -A4, -A8, MAGE-B2, MAGE-D1, -D2, and several members of the GAGE family (GAGE-1, -2, -10, -12, -13) was detected in human testes whereas only MAGE-D1 and MAGE-D2 were expressed in human brain samples (Figures 1(a)–1(c)). Likewise, we detected the expression of Mage-a4, Mage-a 1, 2, 3, 5, 6, 8, Mage-b 1-3, Mage-b3, Mage-d1, -d2 in mouse testes and Mage-d1, -d2 in brains correspondingly (Figures 1(d) and 1(e)). Our analysis has demonstrated that all primers detected only PCR sequences of expected size and did not detect additional nonspecific sequences. In addition, the expression of all CTAs studied was detected only in the testes whereas in normal somatic tissue (brain samples) only MAGE-D1, -D2/Mage-d1, -d2 have expressed as expected.


Expression patterns of cancer-testis antigens in human embryonic stem cells and their cell derivatives indicate lineage tracks.

Lifantseva N, Koltsova A, Krylova T, Yakovleva T, Poljanskaya G, Gordeeva O - Stem Cells Int (2011)

CTA expression in adult human and mouse testicular and brain samples. Designations: T: human testes, B: human brain.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3140037&req=5

fig1: CTA expression in adult human and mouse testicular and brain samples. Designations: T: human testes, B: human brain.
Mentions: At first, we tested the primers for the detection of CTA expression by RT-PCR in normal human and mouse tissues used as positive and negative controls. The expression of MAGE-A2, -A3, -A6, -A4, -A8, MAGE-B2, MAGE-D1, -D2, and several members of the GAGE family (GAGE-1, -2, -10, -12, -13) was detected in human testes whereas only MAGE-D1 and MAGE-D2 were expressed in human brain samples (Figures 1(a)–1(c)). Likewise, we detected the expression of Mage-a4, Mage-a 1, 2, 3, 5, 6, 8, Mage-b 1-3, Mage-b3, Mage-d1, -d2 in mouse testes and Mage-d1, -d2 in brains correspondingly (Figures 1(d) and 1(e)). Our analysis has demonstrated that all primers detected only PCR sequences of expected size and did not detect additional nonspecific sequences. In addition, the expression of all CTAs studied was detected only in the testes whereas in normal somatic tissue (brain samples) only MAGE-D1, -D2/Mage-d1, -d2 have expressed as expected.

Bottom Line: Undifferentiated hES cells and embryoid body cells expressed MAGE-A3, -A6, -A4, -A8, and GAGEs while later differentiated derivatives expressed only MAGE-A8 or MAGE-A4.Despite similarity of the hES and hEC cell expression patterns, MAGE-A2 and MAGE-B2 were detected only in hEC cells but not in hES cells.The identification of CTA expression patterns in pluripotent stem cells and their derivatives may be useful for isolation of abnormally CTA-expressing cells to improve the safety of stem-cell based therapy.

View Article: PubMed Central - PubMed

Affiliation: Kol'tsov Institute of Developmental Biology, Russian Academy of Sciences, 26 Vavilov Street, Moscow 119334, Russia.

ABSTRACT
Pluripotent stem cells can differentiate into various lineages but undergo genetic and epigenetic changes during long-term cultivation and, therefore, require regular monitoring. The expression patterns of cancer-testis antigens (CTAs) MAGE-A2, -A3, -A4, -A6, -A8, -B2, and GAGE were examined in undifferentiated human embryonic stem (hES) cells, their differentiated derivatives, teratocarcinoma (hEC) cells, and cancer cell lines of neuroectodermal and mesodermal origin. Undifferentiated hES cells and embryoid body cells expressed MAGE-A3, -A6, -A4, -A8, and GAGEs while later differentiated derivatives expressed only MAGE-A8 or MAGE-A4. Likewise, mouse pluripotent stem cells also express CTAs of Magea but not Mageb family. Despite similarity of the hES and hEC cell expression patterns, MAGE-A2 and MAGE-B2 were detected only in hEC cells but not in hES cells. Moreover, our analysis has shown that CTAs are aberrantly expressed in cancer cell lines and display low tissue specificity. The identification of CTA expression patterns in pluripotent stem cells and their derivatives may be useful for isolation of abnormally CTA-expressing cells to improve the safety of stem-cell based therapy.

No MeSH data available.


Related in: MedlinePlus