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Identification of Differentially Expressed MicroRNAs in Osteosarcoma.

Lulla RR, Costa FF, Bischof JM, Chou PM, de F Bonaldo M, Vanin EF, Soares MB - Sarcoma (2011)

Bottom Line: In this study, we performed miRNA expression profiling of osteosarcoma cell lines, tumor samples, and normal human osteoblasts.Both miR-135b and miR-150 have been previously shown to be important in cancer.We hypothesize that dysregulation of differentially expressed microRNAs may contribute to tumorigenesis.

View Article: PubMed Central - PubMed

Affiliation: Division of Hematology, Oncology and Stem Cell Transplantation, Children's Memorial Hospital, 2300 Children's Plaza, Chicago, IL 60614, USA.

ABSTRACT
A limited number of reports have investigated the role of microRNAs in osteosarcoma. In this study, we performed miRNA expression profiling of osteosarcoma cell lines, tumor samples, and normal human osteoblasts. Twenty-two differentially expressed microRNAs were identified using high throughput real-time PCR analysis, and 4 (miR-135b, miR-150, miR-542-5p, and miR-652) were confirmed and validated in a different group of tumors. Both miR-135b and miR-150 have been previously shown to be important in cancer. We hypothesize that dysregulation of differentially expressed microRNAs may contribute to tumorigenesis. They might also represent molecular biomarkers or targets for drug development in osteosarcoma.

No MeSH data available.


Related in: MedlinePlus

Heat Map showing unsupervised hierarchical clustering performed using Pearson's correlation (Multiexperiment Viewer, Version 4.5.1).  Relative expression values from normal osteoblasts (HOB), 2 osteosarcoma cell lines (HOS and 143B), and 4 human tumors samples (OS 1, 2, 3, and 5) are represented in the main tree (a).  A higher magnification of the column dendrogram showing that each group clusters together suggesting that the samples have similar miRNA expression profiles (b).
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fig1: Heat Map showing unsupervised hierarchical clustering performed using Pearson's correlation (Multiexperiment Viewer, Version 4.5.1). Relative expression values from normal osteoblasts (HOB), 2 osteosarcoma cell lines (HOS and 143B), and 4 human tumors samples (OS 1, 2, 3, and 5) are represented in the main tree (a). A higher magnification of the column dendrogram showing that each group clusters together suggesting that the samples have similar miRNA expression profiles (b).

Mentions: Real-time qPCR was used to evaluate the relative expression of 762 mature miRNA expression levels in 4 human tumors and 2 osteosarcoma cell lines (HOS and 143B) compared to a normal human osteoblast cell line (HOB). Unsupervised hierarchical clustering was performed using the relative expression values for all samples and all miRNA probes. Based on their expression values, each group (normal osteoblasts, human tumors, and osteosarcoma cell lines) clustered together, thus confirming similar miRNA expression profiles (Figure 1). Additionally, the tumor samples and osteosarcoma cell lines clustered independently from the normal human osteoblasts.


Identification of Differentially Expressed MicroRNAs in Osteosarcoma.

Lulla RR, Costa FF, Bischof JM, Chou PM, de F Bonaldo M, Vanin EF, Soares MB - Sarcoma (2011)

Heat Map showing unsupervised hierarchical clustering performed using Pearson's correlation (Multiexperiment Viewer, Version 4.5.1).  Relative expression values from normal osteoblasts (HOB), 2 osteosarcoma cell lines (HOS and 143B), and 4 human tumors samples (OS 1, 2, 3, and 5) are represented in the main tree (a).  A higher magnification of the column dendrogram showing that each group clusters together suggesting that the samples have similar miRNA expression profiles (b).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3140035&req=5

fig1: Heat Map showing unsupervised hierarchical clustering performed using Pearson's correlation (Multiexperiment Viewer, Version 4.5.1). Relative expression values from normal osteoblasts (HOB), 2 osteosarcoma cell lines (HOS and 143B), and 4 human tumors samples (OS 1, 2, 3, and 5) are represented in the main tree (a). A higher magnification of the column dendrogram showing that each group clusters together suggesting that the samples have similar miRNA expression profiles (b).
Mentions: Real-time qPCR was used to evaluate the relative expression of 762 mature miRNA expression levels in 4 human tumors and 2 osteosarcoma cell lines (HOS and 143B) compared to a normal human osteoblast cell line (HOB). Unsupervised hierarchical clustering was performed using the relative expression values for all samples and all miRNA probes. Based on their expression values, each group (normal osteoblasts, human tumors, and osteosarcoma cell lines) clustered together, thus confirming similar miRNA expression profiles (Figure 1). Additionally, the tumor samples and osteosarcoma cell lines clustered independently from the normal human osteoblasts.

Bottom Line: In this study, we performed miRNA expression profiling of osteosarcoma cell lines, tumor samples, and normal human osteoblasts.Both miR-135b and miR-150 have been previously shown to be important in cancer.We hypothesize that dysregulation of differentially expressed microRNAs may contribute to tumorigenesis.

View Article: PubMed Central - PubMed

Affiliation: Division of Hematology, Oncology and Stem Cell Transplantation, Children's Memorial Hospital, 2300 Children's Plaza, Chicago, IL 60614, USA.

ABSTRACT
A limited number of reports have investigated the role of microRNAs in osteosarcoma. In this study, we performed miRNA expression profiling of osteosarcoma cell lines, tumor samples, and normal human osteoblasts. Twenty-two differentially expressed microRNAs were identified using high throughput real-time PCR analysis, and 4 (miR-135b, miR-150, miR-542-5p, and miR-652) were confirmed and validated in a different group of tumors. Both miR-135b and miR-150 have been previously shown to be important in cancer. We hypothesize that dysregulation of differentially expressed microRNAs may contribute to tumorigenesis. They might also represent molecular biomarkers or targets for drug development in osteosarcoma.

No MeSH data available.


Related in: MedlinePlus