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Mumijo traditional medicine: fossil deposits from antarctica (chemical composition and beneficial bioactivity).

Aiello A, Fattorusso E, Menna M, Vitalone R, Schröder HC, Müller WE - Evid Based Complement Alternat Med (2010)

Bottom Line: Extensive purification and chemical analysis revealed that the fossil samples are a mixture of glycerol derivatives.In addition, the fraction rich in glycerol ethers/wax esters displayed a significant growth-promoting activity in permanent neuronal PC12 cells.It is concluded that this new Mumijo preparation has distinct and marked neuroprotective activity, very likely due to the content of glycerol ether derivatives.

View Article: PubMed Central - PubMed

Affiliation: Dipartimento di Chimica delle Sostanze Naturali, Università di Napoli "Federico II", via D. Montesano 49, 80131 Napoli, Italy.

ABSTRACT
Mumijo is a widely used traditional medicine, especially in Russia, Altai Mountains, Mongolia, Iran Kasachstan and in Kirgistan. Mumijo preparations have been successfully used for the prevention and treatment of infectious diseases; they display immune-stimulating and antiallergic activity as well. In the present study, we investigate the chemical composition and the biomedical potential of a Mumijo(-related) product collected from the Antarctica. The yellow material originates from the snow petrels, Pagodroma nivea. Extensive purification and chemical analysis revealed that the fossil samples are a mixture of glycerol derivatives. In vitro experiments showed that the Mumijo extract caused in cortical neurons a strong neuroprotective effect against the apoptosis-inducing amyloid peptide fragment β-fragment 25-35 (Aβ25-35). In addition, the fraction rich in glycerol ethers/wax esters displayed a significant growth-promoting activity in permanent neuronal PC12 cells. It is concluded that this new Mumijo preparation has distinct and marked neuroprotective activity, very likely due to the content of glycerol ether derivatives.

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Related in: MedlinePlus

Effect of Mumijo on the cell growth of neuronal PC12 cells. (a) Effect of non-purified Mumijo extract on growth of permanent PC12 cells. (b) Fraction D/3, containing glyceryl ether diacetates caused a dose-dependent stimulation of proliferation. Incubation conditions are given under Section 2.
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fig3: Effect of Mumijo on the cell growth of neuronal PC12 cells. (a) Effect of non-purified Mumijo extract on growth of permanent PC12 cells. (b) Fraction D/3, containing glyceryl ether diacetates caused a dose-dependent stimulation of proliferation. Incubation conditions are given under Section 2.

Mentions: The permanent PC12 cell line, a model system for neuronal differentiation [28], was used as a second cell system to assess the biological activity of Mumijo (Figure 3). The non-purified extract displaced no significant growth stimulatory effect between 0.1 and 10 μg/ml. However, after purification, Mumijo fraction D/3 was effective and resulted in a significant stimulation of cell growth. Already at a concentration of 0.3 μg/ml, a significant increase in the growth stimulatory activity could be measured (114.0 ± 6.8%; P < .001), while the maximal growth promoting function was determined between 3.0 and 10.0 μg/ml (139.2 ± 12.3% or 129.2 ± 10.3%, resp.).


Mumijo traditional medicine: fossil deposits from antarctica (chemical composition and beneficial bioactivity).

Aiello A, Fattorusso E, Menna M, Vitalone R, Schröder HC, Müller WE - Evid Based Complement Alternat Med (2010)

Effect of Mumijo on the cell growth of neuronal PC12 cells. (a) Effect of non-purified Mumijo extract on growth of permanent PC12 cells. (b) Fraction D/3, containing glyceryl ether diacetates caused a dose-dependent stimulation of proliferation. Incubation conditions are given under Section 2.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3139983&req=5

fig3: Effect of Mumijo on the cell growth of neuronal PC12 cells. (a) Effect of non-purified Mumijo extract on growth of permanent PC12 cells. (b) Fraction D/3, containing glyceryl ether diacetates caused a dose-dependent stimulation of proliferation. Incubation conditions are given under Section 2.
Mentions: The permanent PC12 cell line, a model system for neuronal differentiation [28], was used as a second cell system to assess the biological activity of Mumijo (Figure 3). The non-purified extract displaced no significant growth stimulatory effect between 0.1 and 10 μg/ml. However, after purification, Mumijo fraction D/3 was effective and resulted in a significant stimulation of cell growth. Already at a concentration of 0.3 μg/ml, a significant increase in the growth stimulatory activity could be measured (114.0 ± 6.8%; P < .001), while the maximal growth promoting function was determined between 3.0 and 10.0 μg/ml (139.2 ± 12.3% or 129.2 ± 10.3%, resp.).

Bottom Line: Extensive purification and chemical analysis revealed that the fossil samples are a mixture of glycerol derivatives.In addition, the fraction rich in glycerol ethers/wax esters displayed a significant growth-promoting activity in permanent neuronal PC12 cells.It is concluded that this new Mumijo preparation has distinct and marked neuroprotective activity, very likely due to the content of glycerol ether derivatives.

View Article: PubMed Central - PubMed

Affiliation: Dipartimento di Chimica delle Sostanze Naturali, Università di Napoli "Federico II", via D. Montesano 49, 80131 Napoli, Italy.

ABSTRACT
Mumijo is a widely used traditional medicine, especially in Russia, Altai Mountains, Mongolia, Iran Kasachstan and in Kirgistan. Mumijo preparations have been successfully used for the prevention and treatment of infectious diseases; they display immune-stimulating and antiallergic activity as well. In the present study, we investigate the chemical composition and the biomedical potential of a Mumijo(-related) product collected from the Antarctica. The yellow material originates from the snow petrels, Pagodroma nivea. Extensive purification and chemical analysis revealed that the fossil samples are a mixture of glycerol derivatives. In vitro experiments showed that the Mumijo extract caused in cortical neurons a strong neuroprotective effect against the apoptosis-inducing amyloid peptide fragment β-fragment 25-35 (Aβ25-35). In addition, the fraction rich in glycerol ethers/wax esters displayed a significant growth-promoting activity in permanent neuronal PC12 cells. It is concluded that this new Mumijo preparation has distinct and marked neuroprotective activity, very likely due to the content of glycerol ether derivatives.

No MeSH data available.


Related in: MedlinePlus