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An antioxidant phytotherapy to rescue neuronal oxidative stress.

Lin Z, Zhu D, Yan Y, Yu B, Wang Q, Shen P, Ruan K - Evid Based Complement Alternat Med (2011)

Bottom Line: The neurological deficit and brain infarction were also significantly elevated by MCAO as compared with sham-operated group.All the brain oxidative stress and damage were significantly attenuated by 7 days pretreatment with the aqueous extract of FBD (250 mg kg(-1), p.o.).In conclusion, FBD was a distinct antioxidant phytotherapy to rescue neuronal oxidative stress, through blocking LPO, restoring endogenous antioxidant system, but not scavenging free radicals.

View Article: PubMed Central - PubMed

Affiliation: Department of Chinese Medicinal Prescription, China Pharmaceutical University, 639 Longmian Avenue, Jiangning University City, Nanjing, Jiangsu 211198, China.

ABSTRACT
Oxidative stress is involved in the pathogenesis of ischemic neuronal injury. A Chinese herbal formula composed of Poria cocos (Chinese name: Fu Ling), Atractylodes macrocephala (Chinese name: Bai Zhu) and Angelica sinensis (Chinese names: Danggui, Dong quai, Donggui; Korean name: Danggwi) (FBD), has been proved to be beneficial in the treatment of cerebral ischemia/reperfusion (I/R).This study was carried out to evaluate the protective effect of FBD against neuronal oxidative stress in vivo and in vitro. Rat I/R were established by middle cerebral artery occlusion (MCAO) for 1 h, followed by 24 h reperfusion. MCAO led to significant depletion in superoxide dismutase and glutathione and rise in lipid peroxidation (LPO) and nitric oxide in brain. The neurological deficit and brain infarction were also significantly elevated by MCAO as compared with sham-operated group. All the brain oxidative stress and damage were significantly attenuated by 7 days pretreatment with the aqueous extract of FBD (250 mg kg(-1), p.o.). Moreover, cerebrospinal fluid sampled from FBD-pretreated rats protected PC12 cells against oxidative insult induced by 0.2 mM hydrogen peroxide, in a concentration and time-dependent manner (IC(50) 10.6%, ET(50) 1.2 h). However, aqueous extract of FBD just slightly scavenged superoxide anion radical generated in xanthine-xanthine oxidase system (IC(50) 2.4 mg ml(-1)) and hydroxyl radical generated in Fenton reaction system (IC(50) 3.6 mg ml(-1)). In conclusion, FBD was a distinct antioxidant phytotherapy to rescue neuronal oxidative stress, through blocking LPO, restoring endogenous antioxidant system, but not scavenging free radicals.

No MeSH data available.


Related in: MedlinePlus

Scavenging activities of aqueous extract of FBD against superoxide anion radical generated in xanthine-XO system and hydroxyl radical generated in Fe2+/H2O2 Fenton reaction system with ascorbic acid as a positive control. Values were means ± SD, n = 6.
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fig3: Scavenging activities of aqueous extract of FBD against superoxide anion radical generated in xanthine-XO system and hydroxyl radical generated in Fe2+/H2O2 Fenton reaction system with ascorbic acid as a positive control. Values were means ± SD, n = 6.

Mentions: Direct free radical scavenging activity by FBD is shown in Figure 3. At concentrations of 0.05–5.0 mg ml−1, FBD exhibited concentration-dependent scavenging activities against O2•− generated in xanthine-XO system and •OH generated in a Fenton reaction system, with IC50 2.4 mg ml−1 and 3.6 mg kg−1, respectively, higher than those of Vit C (IC50 0.01 mg ml−1 and 0.25 mg ml−1, resp.).


An antioxidant phytotherapy to rescue neuronal oxidative stress.

Lin Z, Zhu D, Yan Y, Yu B, Wang Q, Shen P, Ruan K - Evid Based Complement Alternat Med (2011)

Scavenging activities of aqueous extract of FBD against superoxide anion radical generated in xanthine-XO system and hydroxyl radical generated in Fe2+/H2O2 Fenton reaction system with ascorbic acid as a positive control. Values were means ± SD, n = 6.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3139923&req=5

fig3: Scavenging activities of aqueous extract of FBD against superoxide anion radical generated in xanthine-XO system and hydroxyl radical generated in Fe2+/H2O2 Fenton reaction system with ascorbic acid as a positive control. Values were means ± SD, n = 6.
Mentions: Direct free radical scavenging activity by FBD is shown in Figure 3. At concentrations of 0.05–5.0 mg ml−1, FBD exhibited concentration-dependent scavenging activities against O2•− generated in xanthine-XO system and •OH generated in a Fenton reaction system, with IC50 2.4 mg ml−1 and 3.6 mg kg−1, respectively, higher than those of Vit C (IC50 0.01 mg ml−1 and 0.25 mg ml−1, resp.).

Bottom Line: The neurological deficit and brain infarction were also significantly elevated by MCAO as compared with sham-operated group.All the brain oxidative stress and damage were significantly attenuated by 7 days pretreatment with the aqueous extract of FBD (250 mg kg(-1), p.o.).In conclusion, FBD was a distinct antioxidant phytotherapy to rescue neuronal oxidative stress, through blocking LPO, restoring endogenous antioxidant system, but not scavenging free radicals.

View Article: PubMed Central - PubMed

Affiliation: Department of Chinese Medicinal Prescription, China Pharmaceutical University, 639 Longmian Avenue, Jiangning University City, Nanjing, Jiangsu 211198, China.

ABSTRACT
Oxidative stress is involved in the pathogenesis of ischemic neuronal injury. A Chinese herbal formula composed of Poria cocos (Chinese name: Fu Ling), Atractylodes macrocephala (Chinese name: Bai Zhu) and Angelica sinensis (Chinese names: Danggui, Dong quai, Donggui; Korean name: Danggwi) (FBD), has been proved to be beneficial in the treatment of cerebral ischemia/reperfusion (I/R).This study was carried out to evaluate the protective effect of FBD against neuronal oxidative stress in vivo and in vitro. Rat I/R were established by middle cerebral artery occlusion (MCAO) for 1 h, followed by 24 h reperfusion. MCAO led to significant depletion in superoxide dismutase and glutathione and rise in lipid peroxidation (LPO) and nitric oxide in brain. The neurological deficit and brain infarction were also significantly elevated by MCAO as compared with sham-operated group. All the brain oxidative stress and damage were significantly attenuated by 7 days pretreatment with the aqueous extract of FBD (250 mg kg(-1), p.o.). Moreover, cerebrospinal fluid sampled from FBD-pretreated rats protected PC12 cells against oxidative insult induced by 0.2 mM hydrogen peroxide, in a concentration and time-dependent manner (IC(50) 10.6%, ET(50) 1.2 h). However, aqueous extract of FBD just slightly scavenged superoxide anion radical generated in xanthine-xanthine oxidase system (IC(50) 2.4 mg ml(-1)) and hydroxyl radical generated in Fenton reaction system (IC(50) 3.6 mg ml(-1)). In conclusion, FBD was a distinct antioxidant phytotherapy to rescue neuronal oxidative stress, through blocking LPO, restoring endogenous antioxidant system, but not scavenging free radicals.

No MeSH data available.


Related in: MedlinePlus