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Effective Control of Postprandial Glucose Level through Inhibition of Intestinal Alpha Glucosidase by Cymbopogon martinii (Roxb.).

Ghadyale V, Takalikar S, Haldavnekar V, Arvindekar A - Evid Based Complement Alternat Med (2011)

Bottom Line: Intestinal absorption studies using noneverted intestinal sacs, as well as in vivo studies in streptozotocin-induced diabetic rats using oral glucose tolerance with maltose and sucrose load, revealed better inhibition of alpha glucosidase as compared to acarbose.In vivo studies with maltose load of 2 mg and 3 mg/gm body weight showed a noncompetitive pattern of inhibition at 5 mg/kg body weight of CM as against 60 mg/kg body weight of acarbose.Thus CM is more effective alpha glucosidase inhibitor and at lower concentration than acarbose.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry, Shivaji University, Kolhapur 416 004, India.

ABSTRACT
Inhibition of intestinal alpha glucosidase plays a major role in preventing rise in postprandial glucose level in diabetics. Cymbopogon martinii (CM) (family Poaceae) is used in traditional Indian medicine in treatment of diabetes mellitus. The alpha glucosidase inhibitory action of the plant is studied. The active component was separated using hot water extraction of the whole plant powder, differential solvent extraction, and silica gel column chromatography. The 30 : 70 toluene : ethyl acetate fraction showed optimum activity. The silica gel chromatography fraction demonstrated 98, 98, and 68% inhibition for starch, maltose, and sucrose, respectively, at 5 mg/kg body weight of rats. Intestinal absorption studies using noneverted intestinal sacs, as well as in vivo studies in streptozotocin-induced diabetic rats using oral glucose tolerance with maltose and sucrose load, revealed better inhibition of alpha glucosidase as compared to acarbose. Kinetic studies using Lineweaver Burk plot showed mixed to noncompetitive type of inhibition by CM. In vivo studies with maltose load of 2 mg and 3 mg/gm body weight showed a noncompetitive pattern of inhibition at 5 mg/kg body weight of CM as against 60 mg/kg body weight of acarbose. Thus CM is more effective alpha glucosidase inhibitor and at lower concentration than acarbose.

No MeSH data available.


Related in: MedlinePlus

Effect of maltose load (2 mg/g of body weight) on diabetic rats in absence of inhibitor (♦) and in presence of CM (■), acarbose (▲); as well as in normal rats without inhibitor (□) with inhibitor CM (◊), acarbose (∆) (n = 6, *P < 0.05).
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fig3: Effect of maltose load (2 mg/g of body weight) on diabetic rats in absence of inhibitor (♦) and in presence of CM (■), acarbose (▲); as well as in normal rats without inhibitor (□) with inhibitor CM (◊), acarbose (∆) (n = 6, *P < 0.05).

Mentions: The in vivo alpha glucosidase inhibition can be demonstrated by administration of oral maltose and sucrose load. It can be seen from Figure 3 that, as compared to diabetic control, CM shows decrease in the level of blood glucose on maltose load. It should be noted that although CM shows a 98% inhibition in vitro, it demonstrates better lowering of blood glucose, that is, 153 ± 8.6 mg/dL in 2 hours as compared to acarbose. With sucrose as the substrate (Figure 4), acarbose which has very high affinity for sucrose shows lower blood glucose value after two hours. CM which demonstrates about 68% inhibition in vitro shows an increase by 50 ± 7.05 mg/dL as compared to acarbose. In normal animals too, a decrease in the glucose shoot up is seen at 16 min for maltose and sucrose. Administration of CM to normal rats also showed a lowering of postprandial glucose level as compared to the normal rats.


Effective Control of Postprandial Glucose Level through Inhibition of Intestinal Alpha Glucosidase by Cymbopogon martinii (Roxb.).

Ghadyale V, Takalikar S, Haldavnekar V, Arvindekar A - Evid Based Complement Alternat Med (2011)

Effect of maltose load (2 mg/g of body weight) on diabetic rats in absence of inhibitor (♦) and in presence of CM (■), acarbose (▲); as well as in normal rats without inhibitor (□) with inhibitor CM (◊), acarbose (∆) (n = 6, *P < 0.05).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3139892&req=5

fig3: Effect of maltose load (2 mg/g of body weight) on diabetic rats in absence of inhibitor (♦) and in presence of CM (■), acarbose (▲); as well as in normal rats without inhibitor (□) with inhibitor CM (◊), acarbose (∆) (n = 6, *P < 0.05).
Mentions: The in vivo alpha glucosidase inhibition can be demonstrated by administration of oral maltose and sucrose load. It can be seen from Figure 3 that, as compared to diabetic control, CM shows decrease in the level of blood glucose on maltose load. It should be noted that although CM shows a 98% inhibition in vitro, it demonstrates better lowering of blood glucose, that is, 153 ± 8.6 mg/dL in 2 hours as compared to acarbose. With sucrose as the substrate (Figure 4), acarbose which has very high affinity for sucrose shows lower blood glucose value after two hours. CM which demonstrates about 68% inhibition in vitro shows an increase by 50 ± 7.05 mg/dL as compared to acarbose. In normal animals too, a decrease in the glucose shoot up is seen at 16 min for maltose and sucrose. Administration of CM to normal rats also showed a lowering of postprandial glucose level as compared to the normal rats.

Bottom Line: Intestinal absorption studies using noneverted intestinal sacs, as well as in vivo studies in streptozotocin-induced diabetic rats using oral glucose tolerance with maltose and sucrose load, revealed better inhibition of alpha glucosidase as compared to acarbose.In vivo studies with maltose load of 2 mg and 3 mg/gm body weight showed a noncompetitive pattern of inhibition at 5 mg/kg body weight of CM as against 60 mg/kg body weight of acarbose.Thus CM is more effective alpha glucosidase inhibitor and at lower concentration than acarbose.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry, Shivaji University, Kolhapur 416 004, India.

ABSTRACT
Inhibition of intestinal alpha glucosidase plays a major role in preventing rise in postprandial glucose level in diabetics. Cymbopogon martinii (CM) (family Poaceae) is used in traditional Indian medicine in treatment of diabetes mellitus. The alpha glucosidase inhibitory action of the plant is studied. The active component was separated using hot water extraction of the whole plant powder, differential solvent extraction, and silica gel column chromatography. The 30 : 70 toluene : ethyl acetate fraction showed optimum activity. The silica gel chromatography fraction demonstrated 98, 98, and 68% inhibition for starch, maltose, and sucrose, respectively, at 5 mg/kg body weight of rats. Intestinal absorption studies using noneverted intestinal sacs, as well as in vivo studies in streptozotocin-induced diabetic rats using oral glucose tolerance with maltose and sucrose load, revealed better inhibition of alpha glucosidase as compared to acarbose. Kinetic studies using Lineweaver Burk plot showed mixed to noncompetitive type of inhibition by CM. In vivo studies with maltose load of 2 mg and 3 mg/gm body weight showed a noncompetitive pattern of inhibition at 5 mg/kg body weight of CM as against 60 mg/kg body weight of acarbose. Thus CM is more effective alpha glucosidase inhibitor and at lower concentration than acarbose.

No MeSH data available.


Related in: MedlinePlus