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Antibodies that induce phagocytosis of malaria infected erythrocytes: effect of HIV infection and correlation with clinical outcomes.

Ataíde R, Mwapasa V, Molyneux ME, Meshnick SR, Rogerson SJ - PLoS ONE (2011)

Bottom Line: We measured total IgG and phagocytic antibodies against variant surface antigens of placental-type CS2 parasites in 187 secundigravidae (65% HIV infected).In women with placental malaria infection, phagocytic antibodies to CS2(VSA) were decreased in the presence of HIV (p = 0.011) and correlated positively with infant birth weight (coef = 3.57, p = 0.025), whereas total IgG to CS2(VSA) did not.Secundigravidae may be an informative group for identification of correlates of immunity.

View Article: PubMed Central - PubMed

Affiliation: Department of Medicine (RMH/WH), University of Melbourne, Post Office Royal Melbourne Hospital, Melbourne, Victoria, Australia. ric.ataide@gmail.com

ABSTRACT
HIV infection increases the burden of disease of malaria in pregnancy, in part by impairing the development of immunity. We measured total IgG and phagocytic antibodies against variant surface antigens of placental-type CS2 parasites in 187 secundigravidae (65% HIV infected). In women with placental malaria infection, phagocytic antibodies to CS2(VSA) were decreased in the presence of HIV (p = 0.011) and correlated positively with infant birth weight (coef = 3.57, p = 0.025), whereas total IgG to CS2(VSA) did not. Phagocytic antibodies to CS2(VSA) are valuable tools to study acquired immunity to malaria in the context of HIV co-infection. Secundigravidae may be an informative group for identification of correlates of immunity.

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Associations of antibodies measured with HIV and infant birth weight.A) Values for both phagocytic antibodies (circles) and total IgG to CS2VSA (squares) were plotted according to the women's’ HIV status (HIV negative – full symbols; HIV positive – open symbols). Mann-Whitney rank sum tests were performed on HIV positive vs. negative women. *p = 0.010; n.s. ( p = 0.078). Univariate linear regression of total IgG to CS2VSA (B) and phagocytic antibodies (C) and infant birth weight. Both p-values are plotted. Only phagocytic antibodies correlated with infant birth weight (r2 = 0.025, p = 0.034).
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pone-0022491-g001: Associations of antibodies measured with HIV and infant birth weight.A) Values for both phagocytic antibodies (circles) and total IgG to CS2VSA (squares) were plotted according to the women's’ HIV status (HIV negative – full symbols; HIV positive – open symbols). Mann-Whitney rank sum tests were performed on HIV positive vs. negative women. *p = 0.010; n.s. ( p = 0.078). Univariate linear regression of total IgG to CS2VSA (B) and phagocytic antibodies (C) and infant birth weight. Both p-values are plotted. Only phagocytic antibodies correlated with infant birth weight (r2 = 0.025, p = 0.034).

Mentions: We compared antibody levels in women with evidence of placental malaria infection (PM+) and without infection (PM−). For phagocytic antibodies levels were higher in PM+ women (median; IQR 11.79; 5.92–79.45) than in PM− women (6.00; 3.81–8.52; p<0.001). Similarly, Total IgG to CS2VSA was higher in PM+ women (5.35; 1.77–26.07) than PM− women (1.75; 0.45–3.19; p<0.001). Phagocytic antibodies were significantly decreased in HIV infected women (6.72; 4.22–14.78) compared to HIV uninfected women (9.21; 5.36–36.98; p = 0.011), whereas total IgG to CS2VSA showed a non-significant difference between HIV-infected women (2.14; 0.74–6.71) and women who were HIV-negative (2.97; 1.29–26.20; p = 0.079; Figure 1A). Neither phagocytic antibodies nor total IgG to CS2VSA was correlated with maternal haemoglobin levels. Infant birth weight was also not associated with total IgG to CS2VSA (linear regression (LR): coef = 0.007, p = 0.223) (Figure 1B) but it was correlated with phagocytic antibodies (LR: coef = 3.34, p = 0.034) (Figure 1C).


Antibodies that induce phagocytosis of malaria infected erythrocytes: effect of HIV infection and correlation with clinical outcomes.

Ataíde R, Mwapasa V, Molyneux ME, Meshnick SR, Rogerson SJ - PLoS ONE (2011)

Associations of antibodies measured with HIV and infant birth weight.A) Values for both phagocytic antibodies (circles) and total IgG to CS2VSA (squares) were plotted according to the women's’ HIV status (HIV negative – full symbols; HIV positive – open symbols). Mann-Whitney rank sum tests were performed on HIV positive vs. negative women. *p = 0.010; n.s. ( p = 0.078). Univariate linear regression of total IgG to CS2VSA (B) and phagocytic antibodies (C) and infant birth weight. Both p-values are plotted. Only phagocytic antibodies correlated with infant birth weight (r2 = 0.025, p = 0.034).
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Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3139654&req=5

pone-0022491-g001: Associations of antibodies measured with HIV and infant birth weight.A) Values for both phagocytic antibodies (circles) and total IgG to CS2VSA (squares) were plotted according to the women's’ HIV status (HIV negative – full symbols; HIV positive – open symbols). Mann-Whitney rank sum tests were performed on HIV positive vs. negative women. *p = 0.010; n.s. ( p = 0.078). Univariate linear regression of total IgG to CS2VSA (B) and phagocytic antibodies (C) and infant birth weight. Both p-values are plotted. Only phagocytic antibodies correlated with infant birth weight (r2 = 0.025, p = 0.034).
Mentions: We compared antibody levels in women with evidence of placental malaria infection (PM+) and without infection (PM−). For phagocytic antibodies levels were higher in PM+ women (median; IQR 11.79; 5.92–79.45) than in PM− women (6.00; 3.81–8.52; p<0.001). Similarly, Total IgG to CS2VSA was higher in PM+ women (5.35; 1.77–26.07) than PM− women (1.75; 0.45–3.19; p<0.001). Phagocytic antibodies were significantly decreased in HIV infected women (6.72; 4.22–14.78) compared to HIV uninfected women (9.21; 5.36–36.98; p = 0.011), whereas total IgG to CS2VSA showed a non-significant difference between HIV-infected women (2.14; 0.74–6.71) and women who were HIV-negative (2.97; 1.29–26.20; p = 0.079; Figure 1A). Neither phagocytic antibodies nor total IgG to CS2VSA was correlated with maternal haemoglobin levels. Infant birth weight was also not associated with total IgG to CS2VSA (linear regression (LR): coef = 0.007, p = 0.223) (Figure 1B) but it was correlated with phagocytic antibodies (LR: coef = 3.34, p = 0.034) (Figure 1C).

Bottom Line: We measured total IgG and phagocytic antibodies against variant surface antigens of placental-type CS2 parasites in 187 secundigravidae (65% HIV infected).In women with placental malaria infection, phagocytic antibodies to CS2(VSA) were decreased in the presence of HIV (p = 0.011) and correlated positively with infant birth weight (coef = 3.57, p = 0.025), whereas total IgG to CS2(VSA) did not.Secundigravidae may be an informative group for identification of correlates of immunity.

View Article: PubMed Central - PubMed

Affiliation: Department of Medicine (RMH/WH), University of Melbourne, Post Office Royal Melbourne Hospital, Melbourne, Victoria, Australia. ric.ataide@gmail.com

ABSTRACT
HIV infection increases the burden of disease of malaria in pregnancy, in part by impairing the development of immunity. We measured total IgG and phagocytic antibodies against variant surface antigens of placental-type CS2 parasites in 187 secundigravidae (65% HIV infected). In women with placental malaria infection, phagocytic antibodies to CS2(VSA) were decreased in the presence of HIV (p = 0.011) and correlated positively with infant birth weight (coef = 3.57, p = 0.025), whereas total IgG to CS2(VSA) did not. Phagocytic antibodies to CS2(VSA) are valuable tools to study acquired immunity to malaria in the context of HIV co-infection. Secundigravidae may be an informative group for identification of correlates of immunity.

Show MeSH
Related in: MedlinePlus