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Widespread expression of BORIS/CTCFL in normal and cancer cells.

Jones TA, Ogunkolade BW, Szary J, Aarum J, Mumin MA, Patel S, Pieri CA, Sheer D - PLoS ONE (2011)

Bottom Line: Live imaging of cells transiently transfected with GFP tagged BORIS confirmed the nucleolar accumulation of BORIS.While BORIS transcript levels are low compared to CTCF, its protein levels are readily detectable.These findings show that BORIS expression is more widespread than previously believed, and suggest a role for BORIS in nucleolar function.

View Article: PubMed Central - PubMed

Affiliation: Queen Mary University of London, Centre for Neuroscience and Trauma, Blizard Institute, Barts and the London School of Medicine and Dentistry, London, United Kingdom.

ABSTRACT
BORIS (CTCFL) is the paralog of CTCF (CCCTC-binding factor; NM_006565), a ubiquitously expressed DNA-binding protein with diverse roles in gene expression and chromatin organisation. BORIS and CTCF have virtually identical zinc finger domains, yet display major differences in their respective C- and N-terminal regions. Unlike CTCF, BORIS expression has been reported only in the testis and certain malignancies, leading to its classification as a "cancer-testis" antigen. However, the expression pattern of BORIS is both a significant and unresolved question in the field of DNA binding proteins. Here, we identify BORIS in the cytoplasm and nucleus of a wide range of normal and cancer cells. We compare the localization of CTCF and BORIS in the nucleus and demonstrate enrichment of BORIS within the nucleolus, inside the nucleolin core structure and adjacent to fibrillarin in the dense fibrillar component. In contrast, CTCF is not enriched in the nucleolus. Live imaging of cells transiently transfected with GFP tagged BORIS confirmed the nucleolar accumulation of BORIS. While BORIS transcript levels are low compared to CTCF, its protein levels are readily detectable. These findings show that BORIS expression is more widespread than previously believed, and suggest a role for BORIS in nucleolar function.

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Related in: MedlinePlus

BORIS and CTCF expression in normal human tissues.BORIS transcript levels in normal human tissues (First choice® Human Total RNA Survey Panel, Ambion, UK). Error bars represent the standard deviation.
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pone-0022399-g001: BORIS and CTCF expression in normal human tissues.BORIS transcript levels in normal human tissues (First choice® Human Total RNA Survey Panel, Ambion, UK). Error bars represent the standard deviation.

Mentions: To determine the level of BORIS expression in normal human tissues, total RNA was obtained from human adipose, bladder, brain, cervix, colon, esophagus, kidney, liver, ovary, placenta, prostate, skeletal muscle, small intestine, spleen, testis, thymus, thyroid and trachea (Ambion). Real-time RT-PCR showed the highest BORIS transcript levels in the testis (1010 transcripts/µg total RNA), while lower levels were detected in the other tissues (105-108 transcripts/µg total RNA) in agreement with other reports [7] (Fig. 1). We did not detect BORIS in heart or lung, although this may be due to a limitation in the sensitivity of our assay. CTCF transcript levels were relatively constant in all tissues (109–1010 transcripts/µg total RNA) (Fig 1).


Widespread expression of BORIS/CTCFL in normal and cancer cells.

Jones TA, Ogunkolade BW, Szary J, Aarum J, Mumin MA, Patel S, Pieri CA, Sheer D - PLoS ONE (2011)

BORIS and CTCF expression in normal human tissues.BORIS transcript levels in normal human tissues (First choice® Human Total RNA Survey Panel, Ambion, UK). Error bars represent the standard deviation.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3139640&req=5

pone-0022399-g001: BORIS and CTCF expression in normal human tissues.BORIS transcript levels in normal human tissues (First choice® Human Total RNA Survey Panel, Ambion, UK). Error bars represent the standard deviation.
Mentions: To determine the level of BORIS expression in normal human tissues, total RNA was obtained from human adipose, bladder, brain, cervix, colon, esophagus, kidney, liver, ovary, placenta, prostate, skeletal muscle, small intestine, spleen, testis, thymus, thyroid and trachea (Ambion). Real-time RT-PCR showed the highest BORIS transcript levels in the testis (1010 transcripts/µg total RNA), while lower levels were detected in the other tissues (105-108 transcripts/µg total RNA) in agreement with other reports [7] (Fig. 1). We did not detect BORIS in heart or lung, although this may be due to a limitation in the sensitivity of our assay. CTCF transcript levels were relatively constant in all tissues (109–1010 transcripts/µg total RNA) (Fig 1).

Bottom Line: Live imaging of cells transiently transfected with GFP tagged BORIS confirmed the nucleolar accumulation of BORIS.While BORIS transcript levels are low compared to CTCF, its protein levels are readily detectable.These findings show that BORIS expression is more widespread than previously believed, and suggest a role for BORIS in nucleolar function.

View Article: PubMed Central - PubMed

Affiliation: Queen Mary University of London, Centre for Neuroscience and Trauma, Blizard Institute, Barts and the London School of Medicine and Dentistry, London, United Kingdom.

ABSTRACT
BORIS (CTCFL) is the paralog of CTCF (CCCTC-binding factor; NM_006565), a ubiquitously expressed DNA-binding protein with diverse roles in gene expression and chromatin organisation. BORIS and CTCF have virtually identical zinc finger domains, yet display major differences in their respective C- and N-terminal regions. Unlike CTCF, BORIS expression has been reported only in the testis and certain malignancies, leading to its classification as a "cancer-testis" antigen. However, the expression pattern of BORIS is both a significant and unresolved question in the field of DNA binding proteins. Here, we identify BORIS in the cytoplasm and nucleus of a wide range of normal and cancer cells. We compare the localization of CTCF and BORIS in the nucleus and demonstrate enrichment of BORIS within the nucleolus, inside the nucleolin core structure and adjacent to fibrillarin in the dense fibrillar component. In contrast, CTCF is not enriched in the nucleolus. Live imaging of cells transiently transfected with GFP tagged BORIS confirmed the nucleolar accumulation of BORIS. While BORIS transcript levels are low compared to CTCF, its protein levels are readily detectable. These findings show that BORIS expression is more widespread than previously believed, and suggest a role for BORIS in nucleolar function.

Show MeSH
Related in: MedlinePlus