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Identification of changes in wheat (Triticum aestivum L.) seeds proteome in response to anti-trx s gene.

Guo H, Zhang H, Li Y, Ren J, Wang X, Niu H, Yin J - PLoS ONE (2011)

Bottom Line: Transgenic wheat had been formed after the antisense trx s was transferred into wheat, and it had been certified that the expression of trx h decreased in transgenic wheat, and transgenic wheat has high resistance to pre-harvest sprouting.All these differential proteins are involved in regulation of carbohydrates, esters, nucleic acid, proteins and energy metabolism, and biological stress.The quantitative real time PCR results of some differential proteins, such as trx h, heat shock protein 70, α-amylase, β-amylase, glucose-6-phosphate isomerase, 14-3-3 protein, S3-RNase, glyceraldehyde-3-phosphate dehydrogenase, and WRKY transcription factor 6, represented good correlation between transcripts and proteins.

View Article: PubMed Central - PubMed

Affiliation: National Engineering Research Center for Wheat, Henan Agricultural University, Zhengzhou, China.

ABSTRACT

Background: Thioredoxin h (trx h) is closely related to germination of cereal seeds. The cDNA sequences of the thioredoxin s (trx s) gene from Phalaris coerulescens and the thioredoxin h (trx h) gene from wheat are highly homologous, and their expression products have similar biological functions. Transgenic wheat had been formed after the antisense trx s was transferred into wheat, and it had been certified that the expression of trx h decreased in transgenic wheat, and transgenic wheat has high resistance to pre-harvest sprouting.

Methodology/principal findings: Through analyzing the differential proteome of wheat seeds between transgenic wheat and wild type wheat, the mechanism of transgenic wheat seeds having high resistance to pre-harvest sprouting was studied in the present work. There were 36 differential proteins which had been identified by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF-MS). All these differential proteins are involved in regulation of carbohydrates, esters, nucleic acid, proteins and energy metabolism, and biological stress. The quantitative real time PCR results of some differential proteins, such as trx h, heat shock protein 70, α-amylase, β-amylase, glucose-6-phosphate isomerase, 14-3-3 protein, S3-RNase, glyceraldehyde-3-phosphate dehydrogenase, and WRKY transcription factor 6, represented good correlation between transcripts and proteins. The biological functions of many differential proteins are consistent with the proposed role of trx h in wheat seeds.

Conclusions/significance: A possible model for the role of trx h in wheat seeds germination was proposed in this paper. These results will not only play an important role in clarifying the mechanism that transgenic wheat has high resistance to pre-harvest sprouting, but also provide further evidence for the role of trx h in germination of wheat seeds.

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The predicting results of disulfide bonding state of some differential proteins.AA, amino acid sequence; DB_state, predicted disulfide bonding state (1 = disulfide bonded, 0 = not disulfide bonded); DB_conf, confidence of disulfide bonding state prediction (0 = low to 9 = high); DB_bond, position in sequence of a pair of cysteines predicted to be forming a disulfide bridge; Conn_conf, confidence of connectivity assignment given the predicted disulfide bonding state (real value in [0,1]).
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pone-0022255-g004: The predicting results of disulfide bonding state of some differential proteins.AA, amino acid sequence; DB_state, predicted disulfide bonding state (1 = disulfide bonded, 0 = not disulfide bonded); DB_conf, confidence of disulfide bonding state prediction (0 = low to 9 = high); DB_bond, position in sequence of a pair of cysteines predicted to be forming a disulfide bridge; Conn_conf, confidence of connectivity assignment given the predicted disulfide bonding state (real value in [0,1]).

Mentions: Disulfide bridges play a major role in the stabilization of the folding process for some proteins. Prediction of disulfide bridges from sequence alone is therefore useful for the study of structural and functional properties of specific proteins. In addition, knowledge about the disulfide bonding state of cysteines may help the experimental structure determination process and may be useful in other genomic annotation tasks. In this study, the disulfide bonding state of two differential proteins (Puroindoline b and S3-RNase) were predicted with Cysteines Disulfide Bonding State and Connectivity Predictor (http://disulfind.dsi.unifi.it/) [26]. As shown in Figure 4, Puroindoline b and S3-RNase contain 5 and 2 intrachain disulfide bridges, respectively. Therefore, the structural and functional properties of puroindoline b and S3-RNase might be affected directly by trx h.


Identification of changes in wheat (Triticum aestivum L.) seeds proteome in response to anti-trx s gene.

Guo H, Zhang H, Li Y, Ren J, Wang X, Niu H, Yin J - PLoS ONE (2011)

The predicting results of disulfide bonding state of some differential proteins.AA, amino acid sequence; DB_state, predicted disulfide bonding state (1 = disulfide bonded, 0 = not disulfide bonded); DB_conf, confidence of disulfide bonding state prediction (0 = low to 9 = high); DB_bond, position in sequence of a pair of cysteines predicted to be forming a disulfide bridge; Conn_conf, confidence of connectivity assignment given the predicted disulfide bonding state (real value in [0,1]).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3139615&req=5

pone-0022255-g004: The predicting results of disulfide bonding state of some differential proteins.AA, amino acid sequence; DB_state, predicted disulfide bonding state (1 = disulfide bonded, 0 = not disulfide bonded); DB_conf, confidence of disulfide bonding state prediction (0 = low to 9 = high); DB_bond, position in sequence of a pair of cysteines predicted to be forming a disulfide bridge; Conn_conf, confidence of connectivity assignment given the predicted disulfide bonding state (real value in [0,1]).
Mentions: Disulfide bridges play a major role in the stabilization of the folding process for some proteins. Prediction of disulfide bridges from sequence alone is therefore useful for the study of structural and functional properties of specific proteins. In addition, knowledge about the disulfide bonding state of cysteines may help the experimental structure determination process and may be useful in other genomic annotation tasks. In this study, the disulfide bonding state of two differential proteins (Puroindoline b and S3-RNase) were predicted with Cysteines Disulfide Bonding State and Connectivity Predictor (http://disulfind.dsi.unifi.it/) [26]. As shown in Figure 4, Puroindoline b and S3-RNase contain 5 and 2 intrachain disulfide bridges, respectively. Therefore, the structural and functional properties of puroindoline b and S3-RNase might be affected directly by trx h.

Bottom Line: Transgenic wheat had been formed after the antisense trx s was transferred into wheat, and it had been certified that the expression of trx h decreased in transgenic wheat, and transgenic wheat has high resistance to pre-harvest sprouting.All these differential proteins are involved in regulation of carbohydrates, esters, nucleic acid, proteins and energy metabolism, and biological stress.The quantitative real time PCR results of some differential proteins, such as trx h, heat shock protein 70, α-amylase, β-amylase, glucose-6-phosphate isomerase, 14-3-3 protein, S3-RNase, glyceraldehyde-3-phosphate dehydrogenase, and WRKY transcription factor 6, represented good correlation between transcripts and proteins.

View Article: PubMed Central - PubMed

Affiliation: National Engineering Research Center for Wheat, Henan Agricultural University, Zhengzhou, China.

ABSTRACT

Background: Thioredoxin h (trx h) is closely related to germination of cereal seeds. The cDNA sequences of the thioredoxin s (trx s) gene from Phalaris coerulescens and the thioredoxin h (trx h) gene from wheat are highly homologous, and their expression products have similar biological functions. Transgenic wheat had been formed after the antisense trx s was transferred into wheat, and it had been certified that the expression of trx h decreased in transgenic wheat, and transgenic wheat has high resistance to pre-harvest sprouting.

Methodology/principal findings: Through analyzing the differential proteome of wheat seeds between transgenic wheat and wild type wheat, the mechanism of transgenic wheat seeds having high resistance to pre-harvest sprouting was studied in the present work. There were 36 differential proteins which had been identified by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF-MS). All these differential proteins are involved in regulation of carbohydrates, esters, nucleic acid, proteins and energy metabolism, and biological stress. The quantitative real time PCR results of some differential proteins, such as trx h, heat shock protein 70, α-amylase, β-amylase, glucose-6-phosphate isomerase, 14-3-3 protein, S3-RNase, glyceraldehyde-3-phosphate dehydrogenase, and WRKY transcription factor 6, represented good correlation between transcripts and proteins. The biological functions of many differential proteins are consistent with the proposed role of trx h in wheat seeds.

Conclusions/significance: A possible model for the role of trx h in wheat seeds germination was proposed in this paper. These results will not only play an important role in clarifying the mechanism that transgenic wheat has high resistance to pre-harvest sprouting, but also provide further evidence for the role of trx h in germination of wheat seeds.

Show MeSH