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Differential cathelicidin expression in duodenal and gastric biopsies from Tanzanian and German patients.

Rogoll D, Schauber J, Mheta KK, Stich A, Scheppach W - PLoS ONE (2011)

Bottom Line: Also, in the antrum and corpus/fundus regions of the stomach significantly higher cathelicidin transcript levels were measured in Tanzanian compared to German patients.In gastric biopsies from African patients cathelicidin expression was increased in HP positive compared to HP negative subjects.A higher duodenal and gastric cathelicidin expression in African (compared with European) individuals may be due to upregulation by antigenic stimulation and may confer a higher resistance against enteric infections.

View Article: PubMed Central - PubMed

Affiliation: Department of Medicine II, University of Wuerzburg, Wuerzburg, Germany. stuebs_d@medizin.uni-wuerzburg.de

ABSTRACT

Background: Epithelial surfaces such as the gastrointestinal mucosa depend on expression of antimicrobial peptides like cathelicidin for immune defence against pathogens. The mechanisms behind mucosal cathelicidin regulation are incompletely understood.

Methods: Cathelicidin expression was analysed in duodenal, antral and corpus/fundic mucosal biopsies from African and German patients. Additionally, cathelicidin expression was correlated with Helicobacter pylori (HP) infection and the inflammatory status of the mucosa.

Results: High cathelicidin transcript abundance was detected in duodenal biopsies from African subjects. On the contrary, cathelicidin mRNA expression was either undetectable or very low in tissue specimens from German patients. Also, in the antrum and corpus/fundus regions of the stomach significantly higher cathelicidin transcript levels were measured in Tanzanian compared to German patients. In gastric biopsies from African patients cathelicidin expression was increased in HP positive compared to HP negative subjects. Additionally, the inflammatory status measured by IL-8 expression correlated well with the HP infection status.

Conclusions: A higher duodenal and gastric cathelicidin expression in African (compared with European) individuals may be due to upregulation by antigenic stimulation and may confer a higher resistance against enteric infections.

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Related in: MedlinePlus

Expression of cathelicidin mRNA, interleukin-8 (IL-8) and beta-defensin 2 (hbd2) in biopsies.From the gastric antrum (A) and corpus/fundus (B) of African and German patients. Data were correlated with helicobacter pylori (HP) infection status. Levels are normalized to glyceraldehydes-3-phosphate dehydrogenase (GAPDH). Data are expressed as ∼fold change in mRNA transcript levels relative to German subjects. Horizontal bars represent median cathelicidin expression. Cathelicidin and hbd2 levels were significantly increased in HP positive compared with HP negative patients. The inflammatory status measured by IL-8 expression correlated well with the HP infection status.
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pone-0022049-g003: Expression of cathelicidin mRNA, interleukin-8 (IL-8) and beta-defensin 2 (hbd2) in biopsies.From the gastric antrum (A) and corpus/fundus (B) of African and German patients. Data were correlated with helicobacter pylori (HP) infection status. Levels are normalized to glyceraldehydes-3-phosphate dehydrogenase (GAPDH). Data are expressed as ∼fold change in mRNA transcript levels relative to German subjects. Horizontal bars represent median cathelicidin expression. Cathelicidin and hbd2 levels were significantly increased in HP positive compared with HP negative patients. The inflammatory status measured by IL-8 expression correlated well with the HP infection status.

Mentions: In biopsies from African and German patients cathelicidin expression was analysed in relation to helicobacter pylori (HP) infection status: 19/27 african antral biopsies and 7/11 african corpus or fundus biopsies tested positive for HP. In contrast only 1/10 german antral biopsies and 3/20 corpus or fundus biopsies were tested positive for HP in Germany. As controls we also assessed mRNA levels from IL8 and hBD2, which was upregulated by mucosal inflammation. Figure 3A showed that cathelicidin, hBD2 and IL8 mRNA levels were significantly increased in HP positive compared with HP negative subjects. Figure 3B showed the african corpus/fundus biopsies correlated with the HP infections status. We could show that the IL8 as well as the hBD2 expression level correlated well with the HP infection status.


Differential cathelicidin expression in duodenal and gastric biopsies from Tanzanian and German patients.

Rogoll D, Schauber J, Mheta KK, Stich A, Scheppach W - PLoS ONE (2011)

Expression of cathelicidin mRNA, interleukin-8 (IL-8) and beta-defensin 2 (hbd2) in biopsies.From the gastric antrum (A) and corpus/fundus (B) of African and German patients. Data were correlated with helicobacter pylori (HP) infection status. Levels are normalized to glyceraldehydes-3-phosphate dehydrogenase (GAPDH). Data are expressed as ∼fold change in mRNA transcript levels relative to German subjects. Horizontal bars represent median cathelicidin expression. Cathelicidin and hbd2 levels were significantly increased in HP positive compared with HP negative patients. The inflammatory status measured by IL-8 expression correlated well with the HP infection status.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3139610&req=5

pone-0022049-g003: Expression of cathelicidin mRNA, interleukin-8 (IL-8) and beta-defensin 2 (hbd2) in biopsies.From the gastric antrum (A) and corpus/fundus (B) of African and German patients. Data were correlated with helicobacter pylori (HP) infection status. Levels are normalized to glyceraldehydes-3-phosphate dehydrogenase (GAPDH). Data are expressed as ∼fold change in mRNA transcript levels relative to German subjects. Horizontal bars represent median cathelicidin expression. Cathelicidin and hbd2 levels were significantly increased in HP positive compared with HP negative patients. The inflammatory status measured by IL-8 expression correlated well with the HP infection status.
Mentions: In biopsies from African and German patients cathelicidin expression was analysed in relation to helicobacter pylori (HP) infection status: 19/27 african antral biopsies and 7/11 african corpus or fundus biopsies tested positive for HP. In contrast only 1/10 german antral biopsies and 3/20 corpus or fundus biopsies were tested positive for HP in Germany. As controls we also assessed mRNA levels from IL8 and hBD2, which was upregulated by mucosal inflammation. Figure 3A showed that cathelicidin, hBD2 and IL8 mRNA levels were significantly increased in HP positive compared with HP negative subjects. Figure 3B showed the african corpus/fundus biopsies correlated with the HP infections status. We could show that the IL8 as well as the hBD2 expression level correlated well with the HP infection status.

Bottom Line: Also, in the antrum and corpus/fundus regions of the stomach significantly higher cathelicidin transcript levels were measured in Tanzanian compared to German patients.In gastric biopsies from African patients cathelicidin expression was increased in HP positive compared to HP negative subjects.A higher duodenal and gastric cathelicidin expression in African (compared with European) individuals may be due to upregulation by antigenic stimulation and may confer a higher resistance against enteric infections.

View Article: PubMed Central - PubMed

Affiliation: Department of Medicine II, University of Wuerzburg, Wuerzburg, Germany. stuebs_d@medizin.uni-wuerzburg.de

ABSTRACT

Background: Epithelial surfaces such as the gastrointestinal mucosa depend on expression of antimicrobial peptides like cathelicidin for immune defence against pathogens. The mechanisms behind mucosal cathelicidin regulation are incompletely understood.

Methods: Cathelicidin expression was analysed in duodenal, antral and corpus/fundic mucosal biopsies from African and German patients. Additionally, cathelicidin expression was correlated with Helicobacter pylori (HP) infection and the inflammatory status of the mucosa.

Results: High cathelicidin transcript abundance was detected in duodenal biopsies from African subjects. On the contrary, cathelicidin mRNA expression was either undetectable or very low in tissue specimens from German patients. Also, in the antrum and corpus/fundus regions of the stomach significantly higher cathelicidin transcript levels were measured in Tanzanian compared to German patients. In gastric biopsies from African patients cathelicidin expression was increased in HP positive compared to HP negative subjects. Additionally, the inflammatory status measured by IL-8 expression correlated well with the HP infection status.

Conclusions: A higher duodenal and gastric cathelicidin expression in African (compared with European) individuals may be due to upregulation by antigenic stimulation and may confer a higher resistance against enteric infections.

Show MeSH
Related in: MedlinePlus