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Differential cathelicidin expression in duodenal and gastric biopsies from Tanzanian and German patients.

Rogoll D, Schauber J, Mheta KK, Stich A, Scheppach W - PLoS ONE (2011)

Bottom Line: Also, in the antrum and corpus/fundus regions of the stomach significantly higher cathelicidin transcript levels were measured in Tanzanian compared to German patients.In gastric biopsies from African patients cathelicidin expression was increased in HP positive compared to HP negative subjects.A higher duodenal and gastric cathelicidin expression in African (compared with European) individuals may be due to upregulation by antigenic stimulation and may confer a higher resistance against enteric infections.

View Article: PubMed Central - PubMed

Affiliation: Department of Medicine II, University of Wuerzburg, Wuerzburg, Germany. stuebs_d@medizin.uni-wuerzburg.de

ABSTRACT

Background: Epithelial surfaces such as the gastrointestinal mucosa depend on expression of antimicrobial peptides like cathelicidin for immune defence against pathogens. The mechanisms behind mucosal cathelicidin regulation are incompletely understood.

Methods: Cathelicidin expression was analysed in duodenal, antral and corpus/fundic mucosal biopsies from African and German patients. Additionally, cathelicidin expression was correlated with Helicobacter pylori (HP) infection and the inflammatory status of the mucosa.

Results: High cathelicidin transcript abundance was detected in duodenal biopsies from African subjects. On the contrary, cathelicidin mRNA expression was either undetectable or very low in tissue specimens from German patients. Also, in the antrum and corpus/fundus regions of the stomach significantly higher cathelicidin transcript levels were measured in Tanzanian compared to German patients. In gastric biopsies from African patients cathelicidin expression was increased in HP positive compared to HP negative subjects. Additionally, the inflammatory status measured by IL-8 expression correlated well with the HP infection status.

Conclusions: A higher duodenal and gastric cathelicidin expression in African (compared with European) individuals may be due to upregulation by antigenic stimulation and may confer a higher resistance against enteric infections.

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Related in: MedlinePlus

Expression of cathelicidin mRNA in human duodenal biopsies.Duodenal biopsies from 9 German and 12 African patients were analyzed by real-time PCR for cathelicidin mRNA expression. Levels are normalized to glyceraldehydes-3-phosphate dehydrogenase (GAPDH). Data are expressed as ∼fold change in mRNA transcript levels relative to German subjects. Horizontal bars represent median cathelicidin expression.
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pone-0022049-g001: Expression of cathelicidin mRNA in human duodenal biopsies.Duodenal biopsies from 9 German and 12 African patients were analyzed by real-time PCR for cathelicidin mRNA expression. Levels are normalized to glyceraldehydes-3-phosphate dehydrogenase (GAPDH). Data are expressed as ∼fold change in mRNA transcript levels relative to German subjects. Horizontal bars represent median cathelicidin expression.

Mentions: High cathelicidin transcript abundance was detected in the duodenal biopsies of African subjects. On the contrary, cathelicidin mRNA expression was either undetectable or very low in tissue specimens from German patients (Fig. 1). In addition, cathelicidin expression was also assessed in duodenal biopsies from five German travellers who had recently returned from Africa with diarrheal diseases (data not shown). Cathelicidin transcript levels were similarly undetectable or low as in German subjects without a history of overseas travel. Patient data are given in table 1.


Differential cathelicidin expression in duodenal and gastric biopsies from Tanzanian and German patients.

Rogoll D, Schauber J, Mheta KK, Stich A, Scheppach W - PLoS ONE (2011)

Expression of cathelicidin mRNA in human duodenal biopsies.Duodenal biopsies from 9 German and 12 African patients were analyzed by real-time PCR for cathelicidin mRNA expression. Levels are normalized to glyceraldehydes-3-phosphate dehydrogenase (GAPDH). Data are expressed as ∼fold change in mRNA transcript levels relative to German subjects. Horizontal bars represent median cathelicidin expression.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3139610&req=5

pone-0022049-g001: Expression of cathelicidin mRNA in human duodenal biopsies.Duodenal biopsies from 9 German and 12 African patients were analyzed by real-time PCR for cathelicidin mRNA expression. Levels are normalized to glyceraldehydes-3-phosphate dehydrogenase (GAPDH). Data are expressed as ∼fold change in mRNA transcript levels relative to German subjects. Horizontal bars represent median cathelicidin expression.
Mentions: High cathelicidin transcript abundance was detected in the duodenal biopsies of African subjects. On the contrary, cathelicidin mRNA expression was either undetectable or very low in tissue specimens from German patients (Fig. 1). In addition, cathelicidin expression was also assessed in duodenal biopsies from five German travellers who had recently returned from Africa with diarrheal diseases (data not shown). Cathelicidin transcript levels were similarly undetectable or low as in German subjects without a history of overseas travel. Patient data are given in table 1.

Bottom Line: Also, in the antrum and corpus/fundus regions of the stomach significantly higher cathelicidin transcript levels were measured in Tanzanian compared to German patients.In gastric biopsies from African patients cathelicidin expression was increased in HP positive compared to HP negative subjects.A higher duodenal and gastric cathelicidin expression in African (compared with European) individuals may be due to upregulation by antigenic stimulation and may confer a higher resistance against enteric infections.

View Article: PubMed Central - PubMed

Affiliation: Department of Medicine II, University of Wuerzburg, Wuerzburg, Germany. stuebs_d@medizin.uni-wuerzburg.de

ABSTRACT

Background: Epithelial surfaces such as the gastrointestinal mucosa depend on expression of antimicrobial peptides like cathelicidin for immune defence against pathogens. The mechanisms behind mucosal cathelicidin regulation are incompletely understood.

Methods: Cathelicidin expression was analysed in duodenal, antral and corpus/fundic mucosal biopsies from African and German patients. Additionally, cathelicidin expression was correlated with Helicobacter pylori (HP) infection and the inflammatory status of the mucosa.

Results: High cathelicidin transcript abundance was detected in duodenal biopsies from African subjects. On the contrary, cathelicidin mRNA expression was either undetectable or very low in tissue specimens from German patients. Also, in the antrum and corpus/fundus regions of the stomach significantly higher cathelicidin transcript levels were measured in Tanzanian compared to German patients. In gastric biopsies from African patients cathelicidin expression was increased in HP positive compared to HP negative subjects. Additionally, the inflammatory status measured by IL-8 expression correlated well with the HP infection status.

Conclusions: A higher duodenal and gastric cathelicidin expression in African (compared with European) individuals may be due to upregulation by antigenic stimulation and may confer a higher resistance against enteric infections.

Show MeSH
Related in: MedlinePlus