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Horizontal transmission of Candida albicans and evidence of a vaccine response in mice colonized with the fungus.

Cutler JE, Corti M, Lambert P, Ferris M, Xin H - PLoS ONE (2011)

Bottom Line: A weakness of these studies is that, unlike humans, mice do not have a C. albicans GI flora and they lack Candida serum antibodies.Body mass, C. albicans in feces, and fungal-specific serum antibodies were monitored longitudinally.Despite the GI-tract colonization and presence of serum antibodies, the animals made antibodies in response to the Fba immunogen.

View Article: PubMed Central - PubMed

Affiliation: Department of Pediatrics, Louisiana State University Health Sciences Center, New Orleans, Louisiana, United States of America. jcutler@chnola-research.org

ABSTRACT
Disseminated candidiasis is the third leading nosocomial blood stream infection in the United States and is often fatal. We previously showed that disseminated candidiasis was preventable in normal mice by immunization with either a glycopeptide or a peptide synthetic vaccine, both of which were Candida albicans cell wall derived. A weakness of these studies is that, unlike humans, mice do not have a C. albicans GI flora and they lack Candida serum antibodies. We examined the influence of C. albicans GI tract colonization and serum antibodies on mouse vaccination responses to the peptide, Fba, derived from fructose bisphosphate aldolase which has cytosolic and cell wall distributions in the fungus. We evaluated the effect of live C. albicans in drinking water and antimicrobial agents on establishment of Candida colonization of the mouse GI tract. Body mass, C. albicans in feces, and fungal-specific serum antibodies were monitored longitudinally. Unexpectedly, C. albicans colonization occurred in mice that received only antibiotics in their drinking water, provided that the mice were housed in the same room as intentionally colonized mice. The fungal strain in unintentionally colonized mice appeared identical to the strain used for intentional GI-tract colonization. This is the first report of horizontal transmission and spontaneous C. albicans colonization in mice. Importantly, many Candida-colonized mice developed serum fungal-specific antibodies. Despite the GI-tract colonization and presence of serum antibodies, the animals made antibodies in response to the Fba immunogen. This mouse model has potential for elucidating C. albicans horizontal transmission and for exploring factors that induce host defense against disseminated candidiasis. Furthermore, a combined protracted GI-tract colonization with Candida and the possibility of serum antibody responses to the presence of the fungus makes this an attractive mouse model for testing the efficacy of vaccines designed to prevent human disseminated candidiasis.

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Related in: MedlinePlus

Confirmation of horizontal transmission of C. albicans in mice treated with antibacterial agents.Antimicrobial-treated mice not fed C. albicans (groups B, D and H) housed in a common room (Panel A) with other groups of mice intentionally colonized with the fungus (+Ca), began showing evidence of fungal colonization by day 38 (group H) or after day 52 (groups B and D). Duplicate groups of B, D and H kept in an isolated room (i.e., groups B', D' and H', respectively) did not show evidence of the fungus in their feces (Panel B). p = penicillin; s = streptomycin; f = fluconazole; +Ca = C. albicans in drinking water on days 5–9; +Fba = vaccinated with Fba starting on day 31. The various symbols on the curves represent each animal subject.
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pone-0022030-g003: Confirmation of horizontal transmission of C. albicans in mice treated with antibacterial agents.Antimicrobial-treated mice not fed C. albicans (groups B, D and H) housed in a common room (Panel A) with other groups of mice intentionally colonized with the fungus (+Ca), began showing evidence of fungal colonization by day 38 (group H) or after day 52 (groups B and D). Duplicate groups of B, D and H kept in an isolated room (i.e., groups B', D' and H', respectively) did not show evidence of the fungus in their feces (Panel B). p = penicillin; s = streptomycin; f = fluconazole; +Ca = C. albicans in drinking water on days 5–9; +Fba = vaccinated with Fba starting on day 31. The various symbols on the curves represent each animal subject.

Mentions: Antibiotic-treated mice showed an initial weight drop during the first few days as in the previous experiments, but then steadily gained weight until day 80. As in the previous experiment, regardless of whether they were fed C. albicans in the drinking water, all antibiotic-treated mice in the common room developed a fungal colonization of the GI tract by day 66 of the experiment (Fig. 3, panel A: groups A, B, C, D, G, and H).


Horizontal transmission of Candida albicans and evidence of a vaccine response in mice colonized with the fungus.

Cutler JE, Corti M, Lambert P, Ferris M, Xin H - PLoS ONE (2011)

Confirmation of horizontal transmission of C. albicans in mice treated with antibacterial agents.Antimicrobial-treated mice not fed C. albicans (groups B, D and H) housed in a common room (Panel A) with other groups of mice intentionally colonized with the fungus (+Ca), began showing evidence of fungal colonization by day 38 (group H) or after day 52 (groups B and D). Duplicate groups of B, D and H kept in an isolated room (i.e., groups B', D' and H', respectively) did not show evidence of the fungus in their feces (Panel B). p = penicillin; s = streptomycin; f = fluconazole; +Ca = C. albicans in drinking water on days 5–9; +Fba = vaccinated with Fba starting on day 31. The various symbols on the curves represent each animal subject.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3139608&req=5

pone-0022030-g003: Confirmation of horizontal transmission of C. albicans in mice treated with antibacterial agents.Antimicrobial-treated mice not fed C. albicans (groups B, D and H) housed in a common room (Panel A) with other groups of mice intentionally colonized with the fungus (+Ca), began showing evidence of fungal colonization by day 38 (group H) or after day 52 (groups B and D). Duplicate groups of B, D and H kept in an isolated room (i.e., groups B', D' and H', respectively) did not show evidence of the fungus in their feces (Panel B). p = penicillin; s = streptomycin; f = fluconazole; +Ca = C. albicans in drinking water on days 5–9; +Fba = vaccinated with Fba starting on day 31. The various symbols on the curves represent each animal subject.
Mentions: Antibiotic-treated mice showed an initial weight drop during the first few days as in the previous experiments, but then steadily gained weight until day 80. As in the previous experiment, regardless of whether they were fed C. albicans in the drinking water, all antibiotic-treated mice in the common room developed a fungal colonization of the GI tract by day 66 of the experiment (Fig. 3, panel A: groups A, B, C, D, G, and H).

Bottom Line: A weakness of these studies is that, unlike humans, mice do not have a C. albicans GI flora and they lack Candida serum antibodies.Body mass, C. albicans in feces, and fungal-specific serum antibodies were monitored longitudinally.Despite the GI-tract colonization and presence of serum antibodies, the animals made antibodies in response to the Fba immunogen.

View Article: PubMed Central - PubMed

Affiliation: Department of Pediatrics, Louisiana State University Health Sciences Center, New Orleans, Louisiana, United States of America. jcutler@chnola-research.org

ABSTRACT
Disseminated candidiasis is the third leading nosocomial blood stream infection in the United States and is often fatal. We previously showed that disseminated candidiasis was preventable in normal mice by immunization with either a glycopeptide or a peptide synthetic vaccine, both of which were Candida albicans cell wall derived. A weakness of these studies is that, unlike humans, mice do not have a C. albicans GI flora and they lack Candida serum antibodies. We examined the influence of C. albicans GI tract colonization and serum antibodies on mouse vaccination responses to the peptide, Fba, derived from fructose bisphosphate aldolase which has cytosolic and cell wall distributions in the fungus. We evaluated the effect of live C. albicans in drinking water and antimicrobial agents on establishment of Candida colonization of the mouse GI tract. Body mass, C. albicans in feces, and fungal-specific serum antibodies were monitored longitudinally. Unexpectedly, C. albicans colonization occurred in mice that received only antibiotics in their drinking water, provided that the mice were housed in the same room as intentionally colonized mice. The fungal strain in unintentionally colonized mice appeared identical to the strain used for intentional GI-tract colonization. This is the first report of horizontal transmission and spontaneous C. albicans colonization in mice. Importantly, many Candida-colonized mice developed serum fungal-specific antibodies. Despite the GI-tract colonization and presence of serum antibodies, the animals made antibodies in response to the Fba immunogen. This mouse model has potential for elucidating C. albicans horizontal transmission and for exploring factors that induce host defense against disseminated candidiasis. Furthermore, a combined protracted GI-tract colonization with Candida and the possibility of serum antibody responses to the presence of the fungus makes this an attractive mouse model for testing the efficacy of vaccines designed to prevent human disseminated candidiasis.

Show MeSH
Related in: MedlinePlus