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Graph theoretical analysis of functional brain networks: test-retest evaluation on short- and long-term resting-state functional MRI data.

Wang JH, Zuo XN, Gohel S, Milham MP, Biswal BB, He Y - PLoS ONE (2011)

Bottom Line: We found that reliability of global network metrics was overall low, threshold-sensitive and dependent on several factors of scanning time interval (TI, long-term>short-term), network membership (NM, networks excluding negative correlations>networks including negative correlations) and network type (NT, binarized networks>weighted networks).Simulation analysis revealed that global network metrics were extremely sensitive (but varying degrees) to noise in functional connectivity and weighted networks generated numerically more reliable results in compared with binarized networks.For nodal network metrics, they showed high resistance to noise in functional connectivity and no NT related differences were found in the resistance.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Cognitive Neuroscience and Learning, Beijing Normal University, Beijing, China.

ABSTRACT
Graph-based computational network analysis has proven a powerful tool to quantitatively characterize functional architectures of the brain. However, the test-retest (TRT) reliability of graph metrics of functional networks has not been systematically examined. Here, we investigated TRT reliability of topological metrics of functional brain networks derived from resting-state functional magnetic resonance imaging data. Specifically, we evaluated both short-term (<1 hour apart) and long-term (>5 months apart) TRT reliability for 12 global and 6 local nodal network metrics. We found that reliability of global network metrics was overall low, threshold-sensitive and dependent on several factors of scanning time interval (TI, long-term>short-term), network membership (NM, networks excluding negative correlations>networks including negative correlations) and network type (NT, binarized networks>weighted networks). The dependence was modulated by another factor of node definition (ND) strategy. The local nodal reliability exhibited large variability across nodal metrics and a spatially heterogeneous distribution. Nodal degree was the most reliable metric and varied the least across the factors above. Hub regions in association and limbic/paralimbic cortices showed moderate TRT reliability. Importantly, nodal reliability was robust to above-mentioned four factors. Simulation analysis revealed that global network metrics were extremely sensitive (but varying degrees) to noise in functional connectivity and weighted networks generated numerically more reliable results in compared with binarized networks. For nodal network metrics, they showed high resistance to noise in functional connectivity and no NT related differences were found in the resistance. These findings provide important implications on how to choose reliable analytical schemes and network metrics of interest.

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Boxplot of mean nodal TRT reliability for S-AAL- (a) and F-DOS- (b) based networks.Significant differences were found in the mean nodal reliability among the six nodal metrics examined with nodal degree showing the highest ICC values and least variances for both ROIs sets. TRT, test-retest; S-AAL, structural ROIs from Anatomical Automatic Labeling atlas; F-DOS, functional ROIs from Dosenbach et al. (2006, 2010).
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pone-0021976-g008: Boxplot of mean nodal TRT reliability for S-AAL- (a) and F-DOS- (b) based networks.Significant differences were found in the mean nodal reliability among the six nodal metrics examined with nodal degree showing the highest ICC values and least variances for both ROIs sets. TRT, test-retest; S-AAL, structural ROIs from Anatomical Automatic Labeling atlas; F-DOS, functional ROIs from Dosenbach et al. (2006, 2010).

Mentions: Second, nodal reliability exhibited variable patterns across nodal attributes under each combination of the three factors. Further one-factor repeated-measure ANOVA on the mean nodal reliability over regions supported this finding that there was significant (F(5,35) = 6.578, p = 0.0002) differences among the six nodal metrics examined, with the highest ICC values and least variance for nodal degree (Fig. 8a).


Graph theoretical analysis of functional brain networks: test-retest evaluation on short- and long-term resting-state functional MRI data.

Wang JH, Zuo XN, Gohel S, Milham MP, Biswal BB, He Y - PLoS ONE (2011)

Boxplot of mean nodal TRT reliability for S-AAL- (a) and F-DOS- (b) based networks.Significant differences were found in the mean nodal reliability among the six nodal metrics examined with nodal degree showing the highest ICC values and least variances for both ROIs sets. TRT, test-retest; S-AAL, structural ROIs from Anatomical Automatic Labeling atlas; F-DOS, functional ROIs from Dosenbach et al. (2006, 2010).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3139595&req=5

pone-0021976-g008: Boxplot of mean nodal TRT reliability for S-AAL- (a) and F-DOS- (b) based networks.Significant differences were found in the mean nodal reliability among the six nodal metrics examined with nodal degree showing the highest ICC values and least variances for both ROIs sets. TRT, test-retest; S-AAL, structural ROIs from Anatomical Automatic Labeling atlas; F-DOS, functional ROIs from Dosenbach et al. (2006, 2010).
Mentions: Second, nodal reliability exhibited variable patterns across nodal attributes under each combination of the three factors. Further one-factor repeated-measure ANOVA on the mean nodal reliability over regions supported this finding that there was significant (F(5,35) = 6.578, p = 0.0002) differences among the six nodal metrics examined, with the highest ICC values and least variance for nodal degree (Fig. 8a).

Bottom Line: We found that reliability of global network metrics was overall low, threshold-sensitive and dependent on several factors of scanning time interval (TI, long-term>short-term), network membership (NM, networks excluding negative correlations>networks including negative correlations) and network type (NT, binarized networks>weighted networks).Simulation analysis revealed that global network metrics were extremely sensitive (but varying degrees) to noise in functional connectivity and weighted networks generated numerically more reliable results in compared with binarized networks.For nodal network metrics, they showed high resistance to noise in functional connectivity and no NT related differences were found in the resistance.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Cognitive Neuroscience and Learning, Beijing Normal University, Beijing, China.

ABSTRACT
Graph-based computational network analysis has proven a powerful tool to quantitatively characterize functional architectures of the brain. However, the test-retest (TRT) reliability of graph metrics of functional networks has not been systematically examined. Here, we investigated TRT reliability of topological metrics of functional brain networks derived from resting-state functional magnetic resonance imaging data. Specifically, we evaluated both short-term (<1 hour apart) and long-term (>5 months apart) TRT reliability for 12 global and 6 local nodal network metrics. We found that reliability of global network metrics was overall low, threshold-sensitive and dependent on several factors of scanning time interval (TI, long-term>short-term), network membership (NM, networks excluding negative correlations>networks including negative correlations) and network type (NT, binarized networks>weighted networks). The dependence was modulated by another factor of node definition (ND) strategy. The local nodal reliability exhibited large variability across nodal metrics and a spatially heterogeneous distribution. Nodal degree was the most reliable metric and varied the least across the factors above. Hub regions in association and limbic/paralimbic cortices showed moderate TRT reliability. Importantly, nodal reliability was robust to above-mentioned four factors. Simulation analysis revealed that global network metrics were extremely sensitive (but varying degrees) to noise in functional connectivity and weighted networks generated numerically more reliable results in compared with binarized networks. For nodal network metrics, they showed high resistance to noise in functional connectivity and no NT related differences were found in the resistance. These findings provide important implications on how to choose reliable analytical schemes and network metrics of interest.

Show MeSH
Related in: MedlinePlus