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Peritoneal cavity is dominated by IFNγ-secreting CXCR3+ Th1 cells.

Zygmunt BM, Groebe L, Guzman CA - PLoS ONE (2011)

Bottom Line: The peritoneal cavity environment also results in an increased percentage of memory cells producing cytokines.Up-regulation of IFNγ production occurs mostly in CXCR3(+) cells considered as Th1, whereas the up-regulation of IL-4 affects mostly in CXCR3(-) cells which are considered as Th2.We conclude that the peritoneal cavity does not change the Th-lineage of the cells, but that domination of this anatomic niche by Th1 cells rather results from preferential migration to this compartment.

View Article: PubMed Central - PubMed

Affiliation: Department of Vaccinology and Applied Microbiology, Helmholtz Centre for Infection Research, Braunschweig, Germany.

ABSTRACT
The chemokine receptor CXCR3, which was shown to take part in many inflammatory processes, is considered as a Th1 specific marker. Here, we show in a mouse model that CXCR3 expressing CD4(+) cells preferentially migrate to the peritoneal cavity under steady-state conditions. The peritoneal cavity milieu leads to an up-regulated expression of CXCR3. However, blocking of known ligands of this chemokine receptor did not alter the preferential migration. The peritoneal cavity environment also results in an increased percentage of memory cells producing cytokines. Up-regulation of IFNγ production occurs mostly in CXCR3(+) cells considered as Th1, whereas the up-regulation of IL-4 affects mostly in CXCR3(-) cells which are considered as Th2. We conclude that the peritoneal cavity does not change the Th-lineage of the cells, but that domination of this anatomic niche by Th1 cells rather results from preferential migration to this compartment.

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Related in: MedlinePlus

The environment of the PerC results in increased expression of IFNγ by CD4+ T cells.CXCR3+ CD62Llow CD44high, CXCR3− CD62Llow CD44high and CXCR3− CD62Lhigh CD44low CD4+ T cells were sorted from Sp of naïve C57BL/6 mice and stained with CFSE. Then, cells were transferred to naïve recipient animals by i.v. or i.p. rout and re-isolated after 24 h. Cells were re-stimulated with ionomycin, PMA and brefeldinA, stained for (A) IFNγ, (B) IL-4 and (C) IL-17 production and further analyzed by flow cytometry. The percentage of cells producing the different cytokines was subsequently determined. Similar results were obtained in 2 independent experiments. The differences were statistically significant at P<0.01 (*).
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pone-0018032-g008: The environment of the PerC results in increased expression of IFNγ by CD4+ T cells.CXCR3+ CD62Llow CD44high, CXCR3− CD62Llow CD44high and CXCR3− CD62Lhigh CD44low CD4+ T cells were sorted from Sp of naïve C57BL/6 mice and stained with CFSE. Then, cells were transferred to naïve recipient animals by i.v. or i.p. rout and re-isolated after 24 h. Cells were re-stimulated with ionomycin, PMA and brefeldinA, stained for (A) IFNγ, (B) IL-4 and (C) IL-17 production and further analyzed by flow cytometry. The percentage of cells producing the different cytokines was subsequently determined. Similar results were obtained in 2 independent experiments. The differences were statistically significant at P<0.01 (*).

Mentions: To assess if the observed differences are related to the influence of the peritoneal milieu, we sorted CXCR3+ CD62Llow CD44high, CXCR3− CD62Llow CD44high and CXCR3− CD62Lhigh CD44low CD4+ T cells from Sp of naïve C57BL/6 mice. Then, cells were stained with CFSE and subsequently transferred to naïve recipients animals by i.v. or i.p. route. After 24 h cells were re-isolated, re-stimulated with ionomycin, PMA and brefeldinA, stained for IFNγ, IL-4 and IL-17 production and analyzed by flow cytometry. The obtained results showed that the percentage of transferred cells producing a particular cytokine is always higher in case of cells which were re-isolated from PerC than from Sp (Figure 8 A to C).


Peritoneal cavity is dominated by IFNγ-secreting CXCR3+ Th1 cells.

Zygmunt BM, Groebe L, Guzman CA - PLoS ONE (2011)

The environment of the PerC results in increased expression of IFNγ by CD4+ T cells.CXCR3+ CD62Llow CD44high, CXCR3− CD62Llow CD44high and CXCR3− CD62Lhigh CD44low CD4+ T cells were sorted from Sp of naïve C57BL/6 mice and stained with CFSE. Then, cells were transferred to naïve recipient animals by i.v. or i.p. rout and re-isolated after 24 h. Cells were re-stimulated with ionomycin, PMA and brefeldinA, stained for (A) IFNγ, (B) IL-4 and (C) IL-17 production and further analyzed by flow cytometry. The percentage of cells producing the different cytokines was subsequently determined. Similar results were obtained in 2 independent experiments. The differences were statistically significant at P<0.01 (*).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3138734&req=5

pone-0018032-g008: The environment of the PerC results in increased expression of IFNγ by CD4+ T cells.CXCR3+ CD62Llow CD44high, CXCR3− CD62Llow CD44high and CXCR3− CD62Lhigh CD44low CD4+ T cells were sorted from Sp of naïve C57BL/6 mice and stained with CFSE. Then, cells were transferred to naïve recipient animals by i.v. or i.p. rout and re-isolated after 24 h. Cells were re-stimulated with ionomycin, PMA and brefeldinA, stained for (A) IFNγ, (B) IL-4 and (C) IL-17 production and further analyzed by flow cytometry. The percentage of cells producing the different cytokines was subsequently determined. Similar results were obtained in 2 independent experiments. The differences were statistically significant at P<0.01 (*).
Mentions: To assess if the observed differences are related to the influence of the peritoneal milieu, we sorted CXCR3+ CD62Llow CD44high, CXCR3− CD62Llow CD44high and CXCR3− CD62Lhigh CD44low CD4+ T cells from Sp of naïve C57BL/6 mice. Then, cells were stained with CFSE and subsequently transferred to naïve recipients animals by i.v. or i.p. route. After 24 h cells were re-isolated, re-stimulated with ionomycin, PMA and brefeldinA, stained for IFNγ, IL-4 and IL-17 production and analyzed by flow cytometry. The obtained results showed that the percentage of transferred cells producing a particular cytokine is always higher in case of cells which were re-isolated from PerC than from Sp (Figure 8 A to C).

Bottom Line: The peritoneal cavity environment also results in an increased percentage of memory cells producing cytokines.Up-regulation of IFNγ production occurs mostly in CXCR3(+) cells considered as Th1, whereas the up-regulation of IL-4 affects mostly in CXCR3(-) cells which are considered as Th2.We conclude that the peritoneal cavity does not change the Th-lineage of the cells, but that domination of this anatomic niche by Th1 cells rather results from preferential migration to this compartment.

View Article: PubMed Central - PubMed

Affiliation: Department of Vaccinology and Applied Microbiology, Helmholtz Centre for Infection Research, Braunschweig, Germany.

ABSTRACT
The chemokine receptor CXCR3, which was shown to take part in many inflammatory processes, is considered as a Th1 specific marker. Here, we show in a mouse model that CXCR3 expressing CD4(+) cells preferentially migrate to the peritoneal cavity under steady-state conditions. The peritoneal cavity milieu leads to an up-regulated expression of CXCR3. However, blocking of known ligands of this chemokine receptor did not alter the preferential migration. The peritoneal cavity environment also results in an increased percentage of memory cells producing cytokines. Up-regulation of IFNγ production occurs mostly in CXCR3(+) cells considered as Th1, whereas the up-regulation of IL-4 affects mostly in CXCR3(-) cells which are considered as Th2. We conclude that the peritoneal cavity does not change the Th-lineage of the cells, but that domination of this anatomic niche by Th1 cells rather results from preferential migration to this compartment.

Show MeSH
Related in: MedlinePlus