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Peritoneal cavity is dominated by IFNγ-secreting CXCR3+ Th1 cells.

Zygmunt BM, Groebe L, Guzman CA - PLoS ONE (2011)

Bottom Line: The peritoneal cavity environment also results in an increased percentage of memory cells producing cytokines.Up-regulation of IFNγ production occurs mostly in CXCR3(+) cells considered as Th1, whereas the up-regulation of IL-4 affects mostly in CXCR3(-) cells which are considered as Th2.We conclude that the peritoneal cavity does not change the Th-lineage of the cells, but that domination of this anatomic niche by Th1 cells rather results from preferential migration to this compartment.

View Article: PubMed Central - PubMed

Affiliation: Department of Vaccinology and Applied Microbiology, Helmholtz Centre for Infection Research, Braunschweig, Germany.

ABSTRACT
The chemokine receptor CXCR3, which was shown to take part in many inflammatory processes, is considered as a Th1 specific marker. Here, we show in a mouse model that CXCR3 expressing CD4(+) cells preferentially migrate to the peritoneal cavity under steady-state conditions. The peritoneal cavity milieu leads to an up-regulated expression of CXCR3. However, blocking of known ligands of this chemokine receptor did not alter the preferential migration. The peritoneal cavity environment also results in an increased percentage of memory cells producing cytokines. Up-regulation of IFNγ production occurs mostly in CXCR3(+) cells considered as Th1, whereas the up-regulation of IL-4 affects mostly in CXCR3(-) cells which are considered as Th2. We conclude that the peritoneal cavity does not change the Th-lineage of the cells, but that domination of this anatomic niche by Th1 cells rather results from preferential migration to this compartment.

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Peritoneal memory CD4+ T cells produce more cytokines than Sp derived cells.CXCR3+ CD62Llow CD44high, CXCR3− CD62Llow CD44high and CXCR3− CD62Lhigh CD44low CD4+ T cells were sorted from Sp or PerC of C57BL/6 naïve (specific pathogen free) mice. Then, cells were re-stimulated with ionomycin and PMA and the expression of (A) IFNγ, (B) IL-4 and (C) IL-17 was measured by ELISA. Similar results were obtained in 2 independent experiments.
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pone-0018032-g007: Peritoneal memory CD4+ T cells produce more cytokines than Sp derived cells.CXCR3+ CD62Llow CD44high, CXCR3− CD62Llow CD44high and CXCR3− CD62Lhigh CD44low CD4+ T cells were sorted from Sp or PerC of C57BL/6 naïve (specific pathogen free) mice. Then, cells were re-stimulated with ionomycin and PMA and the expression of (A) IFNγ, (B) IL-4 and (C) IL-17 was measured by ELISA. Similar results were obtained in 2 independent experiments.

Mentions: Taking into account that exposition to the peritoneal milieu results in changes in the expression levels of certain molecules, a comparative evaluation of the cytokines produced by peritoneal and Sp cells was performed. We sorted CXCR3+ CD62Llow CD44high, CXCR3− CD62Llow CD44high and CXCR3− CD62Lhigh CD44low CD4+ cells. The levels of cytokine production were then measured by ELISA in culture supernatants of stimulated cells (Figure 7). The obtained results showed that, as expected, IFNγ is produced by CXCR3+ cells, whereas IL-4 and IL-17 are mostly expressed by CXCR3- cells. When the expression levels of the two populations were compared, by and large cells isolated from the PerC showed the highest expression levels.


Peritoneal cavity is dominated by IFNγ-secreting CXCR3+ Th1 cells.

Zygmunt BM, Groebe L, Guzman CA - PLoS ONE (2011)

Peritoneal memory CD4+ T cells produce more cytokines than Sp derived cells.CXCR3+ CD62Llow CD44high, CXCR3− CD62Llow CD44high and CXCR3− CD62Lhigh CD44low CD4+ T cells were sorted from Sp or PerC of C57BL/6 naïve (specific pathogen free) mice. Then, cells were re-stimulated with ionomycin and PMA and the expression of (A) IFNγ, (B) IL-4 and (C) IL-17 was measured by ELISA. Similar results were obtained in 2 independent experiments.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3138734&req=5

pone-0018032-g007: Peritoneal memory CD4+ T cells produce more cytokines than Sp derived cells.CXCR3+ CD62Llow CD44high, CXCR3− CD62Llow CD44high and CXCR3− CD62Lhigh CD44low CD4+ T cells were sorted from Sp or PerC of C57BL/6 naïve (specific pathogen free) mice. Then, cells were re-stimulated with ionomycin and PMA and the expression of (A) IFNγ, (B) IL-4 and (C) IL-17 was measured by ELISA. Similar results were obtained in 2 independent experiments.
Mentions: Taking into account that exposition to the peritoneal milieu results in changes in the expression levels of certain molecules, a comparative evaluation of the cytokines produced by peritoneal and Sp cells was performed. We sorted CXCR3+ CD62Llow CD44high, CXCR3− CD62Llow CD44high and CXCR3− CD62Lhigh CD44low CD4+ cells. The levels of cytokine production were then measured by ELISA in culture supernatants of stimulated cells (Figure 7). The obtained results showed that, as expected, IFNγ is produced by CXCR3+ cells, whereas IL-4 and IL-17 are mostly expressed by CXCR3- cells. When the expression levels of the two populations were compared, by and large cells isolated from the PerC showed the highest expression levels.

Bottom Line: The peritoneal cavity environment also results in an increased percentage of memory cells producing cytokines.Up-regulation of IFNγ production occurs mostly in CXCR3(+) cells considered as Th1, whereas the up-regulation of IL-4 affects mostly in CXCR3(-) cells which are considered as Th2.We conclude that the peritoneal cavity does not change the Th-lineage of the cells, but that domination of this anatomic niche by Th1 cells rather results from preferential migration to this compartment.

View Article: PubMed Central - PubMed

Affiliation: Department of Vaccinology and Applied Microbiology, Helmholtz Centre for Infection Research, Braunschweig, Germany.

ABSTRACT
The chemokine receptor CXCR3, which was shown to take part in many inflammatory processes, is considered as a Th1 specific marker. Here, we show in a mouse model that CXCR3 expressing CD4(+) cells preferentially migrate to the peritoneal cavity under steady-state conditions. The peritoneal cavity milieu leads to an up-regulated expression of CXCR3. However, blocking of known ligands of this chemokine receptor did not alter the preferential migration. The peritoneal cavity environment also results in an increased percentage of memory cells producing cytokines. Up-regulation of IFNγ production occurs mostly in CXCR3(+) cells considered as Th1, whereas the up-regulation of IL-4 affects mostly in CXCR3(-) cells which are considered as Th2. We conclude that the peritoneal cavity does not change the Th-lineage of the cells, but that domination of this anatomic niche by Th1 cells rather results from preferential migration to this compartment.

Show MeSH
Related in: MedlinePlus