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Peritoneal cavity is dominated by IFNγ-secreting CXCR3+ Th1 cells.

Zygmunt BM, Groebe L, Guzman CA - PLoS ONE (2011)

Bottom Line: The peritoneal cavity environment also results in an increased percentage of memory cells producing cytokines.Up-regulation of IFNγ production occurs mostly in CXCR3(+) cells considered as Th1, whereas the up-regulation of IL-4 affects mostly in CXCR3(-) cells which are considered as Th2.We conclude that the peritoneal cavity does not change the Th-lineage of the cells, but that domination of this anatomic niche by Th1 cells rather results from preferential migration to this compartment.

View Article: PubMed Central - PubMed

Affiliation: Department of Vaccinology and Applied Microbiology, Helmholtz Centre for Infection Research, Braunschweig, Germany.

ABSTRACT
The chemokine receptor CXCR3, which was shown to take part in many inflammatory processes, is considered as a Th1 specific marker. Here, we show in a mouse model that CXCR3 expressing CD4(+) cells preferentially migrate to the peritoneal cavity under steady-state conditions. The peritoneal cavity milieu leads to an up-regulated expression of CXCR3. However, blocking of known ligands of this chemokine receptor did not alter the preferential migration. The peritoneal cavity environment also results in an increased percentage of memory cells producing cytokines. Up-regulation of IFNγ production occurs mostly in CXCR3(+) cells considered as Th1, whereas the up-regulation of IL-4 affects mostly in CXCR3(-) cells which are considered as Th2. We conclude that the peritoneal cavity does not change the Th-lineage of the cells, but that domination of this anatomic niche by Th1 cells rather results from preferential migration to this compartment.

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Th cell subsets are differentially distributed in different anatomic niches.The percentage of different Th cell subsets in CD4+ cell population were determined by FACS analysis in different organs (n = 5). (A) Th1 cells (IFNγ secreting cells), (B) Th2 cells (IL-4 secreting cells) and (C) Th17 cells (IL-17 secreting cells). The differences were statistically significant at P<0.001 (*). Similar results were obtained in 2 independent experiments. NALT, nasal associated lymphoid tissues; cLN, cervical lymph node; brLN, bronchial lymph nodes; mLN, mesenteric lymph node; PP, Peyer's patches; Sp, spleen; PerC, peritoneal cavity.
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pone-0018032-g006: Th cell subsets are differentially distributed in different anatomic niches.The percentage of different Th cell subsets in CD4+ cell population were determined by FACS analysis in different organs (n = 5). (A) Th1 cells (IFNγ secreting cells), (B) Th2 cells (IL-4 secreting cells) and (C) Th17 cells (IL-17 secreting cells). The differences were statistically significant at P<0.001 (*). Similar results were obtained in 2 independent experiments. NALT, nasal associated lymphoid tissues; cLN, cervical lymph node; brLN, bronchial lymph nodes; mLN, mesenteric lymph node; PP, Peyer's patches; Sp, spleen; PerC, peritoneal cavity.

Mentions: Our results showed that in comparison to other anatomic niches, the PerC is dominated by CXCR3+ CD4+ cells. IFNγ is mostly produced by CXCR3+ CD4+ cells, thereby suggesting that this compartment is predominantly populated by Th1 cells. To validate this hypothesis, cells isolated from different organs were stained for cytokines and analyzed by flow cytometry. Our hypothesis was fully confirmed by the obtained experimental data (Figure 6A). The proportion of IFNγ secreting cells present in the PerC is many times higher than in any other organ tested. Of remark, the proportion of Th1 cells in the PerC is also much higher in comparison to lungs, another peripheral territory tested. We also assessed the expression of other cytokines by peritoneal CD4+ cells (Figure 6B and C). It was observed that the percentage of cells expressing IL-4 or IL-17 is also higher in the PerC than in other organs, however, the differences are not as prominent as for IFNγ.


Peritoneal cavity is dominated by IFNγ-secreting CXCR3+ Th1 cells.

Zygmunt BM, Groebe L, Guzman CA - PLoS ONE (2011)

Th cell subsets are differentially distributed in different anatomic niches.The percentage of different Th cell subsets in CD4+ cell population were determined by FACS analysis in different organs (n = 5). (A) Th1 cells (IFNγ secreting cells), (B) Th2 cells (IL-4 secreting cells) and (C) Th17 cells (IL-17 secreting cells). The differences were statistically significant at P<0.001 (*). Similar results were obtained in 2 independent experiments. NALT, nasal associated lymphoid tissues; cLN, cervical lymph node; brLN, bronchial lymph nodes; mLN, mesenteric lymph node; PP, Peyer's patches; Sp, spleen; PerC, peritoneal cavity.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3138734&req=5

pone-0018032-g006: Th cell subsets are differentially distributed in different anatomic niches.The percentage of different Th cell subsets in CD4+ cell population were determined by FACS analysis in different organs (n = 5). (A) Th1 cells (IFNγ secreting cells), (B) Th2 cells (IL-4 secreting cells) and (C) Th17 cells (IL-17 secreting cells). The differences were statistically significant at P<0.001 (*). Similar results were obtained in 2 independent experiments. NALT, nasal associated lymphoid tissues; cLN, cervical lymph node; brLN, bronchial lymph nodes; mLN, mesenteric lymph node; PP, Peyer's patches; Sp, spleen; PerC, peritoneal cavity.
Mentions: Our results showed that in comparison to other anatomic niches, the PerC is dominated by CXCR3+ CD4+ cells. IFNγ is mostly produced by CXCR3+ CD4+ cells, thereby suggesting that this compartment is predominantly populated by Th1 cells. To validate this hypothesis, cells isolated from different organs were stained for cytokines and analyzed by flow cytometry. Our hypothesis was fully confirmed by the obtained experimental data (Figure 6A). The proportion of IFNγ secreting cells present in the PerC is many times higher than in any other organ tested. Of remark, the proportion of Th1 cells in the PerC is also much higher in comparison to lungs, another peripheral territory tested. We also assessed the expression of other cytokines by peritoneal CD4+ cells (Figure 6B and C). It was observed that the percentage of cells expressing IL-4 or IL-17 is also higher in the PerC than in other organs, however, the differences are not as prominent as for IFNγ.

Bottom Line: The peritoneal cavity environment also results in an increased percentage of memory cells producing cytokines.Up-regulation of IFNγ production occurs mostly in CXCR3(+) cells considered as Th1, whereas the up-regulation of IL-4 affects mostly in CXCR3(-) cells which are considered as Th2.We conclude that the peritoneal cavity does not change the Th-lineage of the cells, but that domination of this anatomic niche by Th1 cells rather results from preferential migration to this compartment.

View Article: PubMed Central - PubMed

Affiliation: Department of Vaccinology and Applied Microbiology, Helmholtz Centre for Infection Research, Braunschweig, Germany.

ABSTRACT
The chemokine receptor CXCR3, which was shown to take part in many inflammatory processes, is considered as a Th1 specific marker. Here, we show in a mouse model that CXCR3 expressing CD4(+) cells preferentially migrate to the peritoneal cavity under steady-state conditions. The peritoneal cavity milieu leads to an up-regulated expression of CXCR3. However, blocking of known ligands of this chemokine receptor did not alter the preferential migration. The peritoneal cavity environment also results in an increased percentage of memory cells producing cytokines. Up-regulation of IFNγ production occurs mostly in CXCR3(+) cells considered as Th1, whereas the up-regulation of IL-4 affects mostly in CXCR3(-) cells which are considered as Th2. We conclude that the peritoneal cavity does not change the Th-lineage of the cells, but that domination of this anatomic niche by Th1 cells rather results from preferential migration to this compartment.

Show MeSH
Related in: MedlinePlus