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Comparative Cytogenetics Analysis of Chlamys farreri, Patinopecten yessoensis, and Argopecten irradians with Ct-1 DNA by Fluorescence In Situ Hybridization.

Hu LP, Shang WC, Sun Y, Wang S, Ren XL, Huang XT, Bao ZM - Evid Based Complement Alternat Med (2011)

Bottom Line: The results showed that C(0)t-1 DNA signals spread on all chromosomes in the three scallops, whereas signal density and intensity were different strikingly.Based on the C(0)t-1 DNA fluorescent bands, a more exact karyotype of C. farreri has been obtained.In this study, the comparative analysis based on C(0)t-1 DNA provides a new insight into the chromosomal reconstructions during the evolution process, and it is helpful for understanding an important source of genomic diversity, species relationships, and genome evolution.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Marine Genetics and Breeding (MGB), College of Marine Life Sciences, Ocean University of China, Ministry of Education, Qingdao 266003, China.

ABSTRACT
The chromosomes of Chlamys farreri, Patinopecten yessoensis, and Argopecten irradians were studied by FISH using C. farreri C(0)t-1 DNA probes. The results showed that C(0)t-1 DNA signals spread on all chromosomes in the three scallops, whereas signal density and intensity were different strikingly. Clustering brighter signals presented in the centromeric and telomeric regions of most C. farreri chromosomes, and in the centromeric or pericentromeric regions of several P. yessoensis chromosomes. Comparative analysis of the mapping indicated a relatively higher homology in the repetitive DNA sequences of the genome between C. farreri and P. yessoensis than that between C. farreri and A. irradians. In addition, FISH showed that the distribution of C(0)t-1 DNA clustering signals in C. farreri displayed completely similar signal bands between homologous chromosomes. Based on the C(0)t-1 DNA fluorescent bands, a more exact karyotype of C. farreri has been obtained. In this study, the comparative analysis based on C(0)t-1 DNA provides a new insight into the chromosomal reconstructions during the evolution process, and it is helpful for understanding an important source of genomic diversity, species relationships, and genome evolution.

No MeSH data available.


Karyotype of C. farreri and corresponding C0t-1 DNA signal bands.
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Related In: Results  -  Collection


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fig3: Karyotype of C. farreri and corresponding C0t-1 DNA signal bands.

Mentions: The labeled CF C0t-1 DNA was hybridized to the chromosome spreads of C. farreri, P. yessoensis, and A. irradians. The results were shown in Figure 2. On the metaphase chromosomes of C. farreri, the signals of CF C0t-1 DNA presented in all chromosomes, and the clustering brighter signals of CF C0t-1 DNA distributed mainly in areas of centromeres, subcentromeres, and near telomeres, and fewer in the middle regions of chromosome arms (Figures 2(a) and 2(g)). In detail, on three pairs of metacentric chromosomes, two pairs of submetacentric chromosome and one pair of subtelocentric chromosomes, the brighter CF C0t-1 DNA signals mainly concentrated in areas of centromere; on the other three pairs of submetacentric chromosomes and nine pairs of subtelocentric chromosomes, more intensive CF C0t-1 DNA distributed in areas of centromeres, subcentromeres, and telomeric regions of the long arms; and on another pair of subtelocentric chromosomes, more intensive CF C0t-1 DNA distributed in telomeric regions of the long arms. From the characteristics of the signals distribution, the repetitive sequences had specific, strong and steady signal bands on chromosomes, and the homologous chromosomes exhibited similar signal bands, so that karyotype analysis could be conducted based on CF C0t-1 DNA specific fluorescence bands in C. farreri. And the karyotype result was shown in Figure 3. Based on the karyotype picture, the signals were much brighter in centromeres areas of the chromosome pairs 1, 5, 7, 9, 10, 16 and 18, and telomeric regions on the long arms of the chromosome pairs 4, 8, 13, 16, 17, 18 and 19 (Figure 3). On the metaphase chromosomes of P. yessoensis, the signals of CF C0t-1 DNA were also detected in all chromosomes, whereas, the brighter clustering signals of CF C0t-1 DNA could be seen on several chromosomes at centromeric and pericentromeric regions, or near centromeric regions of the long arms (Figures 2(b) and 2(h)). Furthermore, on one or two pairs of subtelocentric chromosomes, the brighter CF C0t-1 DNA signals mainly concentrated in areas of centromeric and pericentromeric regions; on another pair of subtelocentric chromosomes, more intensive CF C0t-1 DNA distributed near centromeric regions of the long arms; on the remaining chromosomes, the signals of CF C0t-1 DNA were dispersed. In contrast, no obviously clustering brighter signals of CF C0t-1 DNA regions were shown and the signals were dispersed on chromosomes of A. irradians (Figures 2(c) and 2(i)).


Comparative Cytogenetics Analysis of Chlamys farreri, Patinopecten yessoensis, and Argopecten irradians with Ct-1 DNA by Fluorescence In Situ Hybridization.

Hu LP, Shang WC, Sun Y, Wang S, Ren XL, Huang XT, Bao ZM - Evid Based Complement Alternat Med (2011)

Karyotype of C. farreri and corresponding C0t-1 DNA signal bands.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3138726&req=5

fig3: Karyotype of C. farreri and corresponding C0t-1 DNA signal bands.
Mentions: The labeled CF C0t-1 DNA was hybridized to the chromosome spreads of C. farreri, P. yessoensis, and A. irradians. The results were shown in Figure 2. On the metaphase chromosomes of C. farreri, the signals of CF C0t-1 DNA presented in all chromosomes, and the clustering brighter signals of CF C0t-1 DNA distributed mainly in areas of centromeres, subcentromeres, and near telomeres, and fewer in the middle regions of chromosome arms (Figures 2(a) and 2(g)). In detail, on three pairs of metacentric chromosomes, two pairs of submetacentric chromosome and one pair of subtelocentric chromosomes, the brighter CF C0t-1 DNA signals mainly concentrated in areas of centromere; on the other three pairs of submetacentric chromosomes and nine pairs of subtelocentric chromosomes, more intensive CF C0t-1 DNA distributed in areas of centromeres, subcentromeres, and telomeric regions of the long arms; and on another pair of subtelocentric chromosomes, more intensive CF C0t-1 DNA distributed in telomeric regions of the long arms. From the characteristics of the signals distribution, the repetitive sequences had specific, strong and steady signal bands on chromosomes, and the homologous chromosomes exhibited similar signal bands, so that karyotype analysis could be conducted based on CF C0t-1 DNA specific fluorescence bands in C. farreri. And the karyotype result was shown in Figure 3. Based on the karyotype picture, the signals were much brighter in centromeres areas of the chromosome pairs 1, 5, 7, 9, 10, 16 and 18, and telomeric regions on the long arms of the chromosome pairs 4, 8, 13, 16, 17, 18 and 19 (Figure 3). On the metaphase chromosomes of P. yessoensis, the signals of CF C0t-1 DNA were also detected in all chromosomes, whereas, the brighter clustering signals of CF C0t-1 DNA could be seen on several chromosomes at centromeric and pericentromeric regions, or near centromeric regions of the long arms (Figures 2(b) and 2(h)). Furthermore, on one or two pairs of subtelocentric chromosomes, the brighter CF C0t-1 DNA signals mainly concentrated in areas of centromeric and pericentromeric regions; on another pair of subtelocentric chromosomes, more intensive CF C0t-1 DNA distributed near centromeric regions of the long arms; on the remaining chromosomes, the signals of CF C0t-1 DNA were dispersed. In contrast, no obviously clustering brighter signals of CF C0t-1 DNA regions were shown and the signals were dispersed on chromosomes of A. irradians (Figures 2(c) and 2(i)).

Bottom Line: The results showed that C(0)t-1 DNA signals spread on all chromosomes in the three scallops, whereas signal density and intensity were different strikingly.Based on the C(0)t-1 DNA fluorescent bands, a more exact karyotype of C. farreri has been obtained.In this study, the comparative analysis based on C(0)t-1 DNA provides a new insight into the chromosomal reconstructions during the evolution process, and it is helpful for understanding an important source of genomic diversity, species relationships, and genome evolution.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Marine Genetics and Breeding (MGB), College of Marine Life Sciences, Ocean University of China, Ministry of Education, Qingdao 266003, China.

ABSTRACT
The chromosomes of Chlamys farreri, Patinopecten yessoensis, and Argopecten irradians were studied by FISH using C. farreri C(0)t-1 DNA probes. The results showed that C(0)t-1 DNA signals spread on all chromosomes in the three scallops, whereas signal density and intensity were different strikingly. Clustering brighter signals presented in the centromeric and telomeric regions of most C. farreri chromosomes, and in the centromeric or pericentromeric regions of several P. yessoensis chromosomes. Comparative analysis of the mapping indicated a relatively higher homology in the repetitive DNA sequences of the genome between C. farreri and P. yessoensis than that between C. farreri and A. irradians. In addition, FISH showed that the distribution of C(0)t-1 DNA clustering signals in C. farreri displayed completely similar signal bands between homologous chromosomes. Based on the C(0)t-1 DNA fluorescent bands, a more exact karyotype of C. farreri has been obtained. In this study, the comparative analysis based on C(0)t-1 DNA provides a new insight into the chromosomal reconstructions during the evolution process, and it is helpful for understanding an important source of genomic diversity, species relationships, and genome evolution.

No MeSH data available.