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Comparative Cytogenetics Analysis of Chlamys farreri, Patinopecten yessoensis, and Argopecten irradians with Ct-1 DNA by Fluorescence In Situ Hybridization.

Hu LP, Shang WC, Sun Y, Wang S, Ren XL, Huang XT, Bao ZM - Evid Based Complement Alternat Med (2011)

Bottom Line: The results showed that C(0)t-1 DNA signals spread on all chromosomes in the three scallops, whereas signal density and intensity were different strikingly.Based on the C(0)t-1 DNA fluorescent bands, a more exact karyotype of C. farreri has been obtained.In this study, the comparative analysis based on C(0)t-1 DNA provides a new insight into the chromosomal reconstructions during the evolution process, and it is helpful for understanding an important source of genomic diversity, species relationships, and genome evolution.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Marine Genetics and Breeding (MGB), College of Marine Life Sciences, Ocean University of China, Ministry of Education, Qingdao 266003, China.

ABSTRACT
The chromosomes of Chlamys farreri, Patinopecten yessoensis, and Argopecten irradians were studied by FISH using C. farreri C(0)t-1 DNA probes. The results showed that C(0)t-1 DNA signals spread on all chromosomes in the three scallops, whereas signal density and intensity were different strikingly. Clustering brighter signals presented in the centromeric and telomeric regions of most C. farreri chromosomes, and in the centromeric or pericentromeric regions of several P. yessoensis chromosomes. Comparative analysis of the mapping indicated a relatively higher homology in the repetitive DNA sequences of the genome between C. farreri and P. yessoensis than that between C. farreri and A. irradians. In addition, FISH showed that the distribution of C(0)t-1 DNA clustering signals in C. farreri displayed completely similar signal bands between homologous chromosomes. Based on the C(0)t-1 DNA fluorescent bands, a more exact karyotype of C. farreri has been obtained. In this study, the comparative analysis based on C(0)t-1 DNA provides a new insight into the chromosomal reconstructions during the evolution process, and it is helpful for understanding an important source of genomic diversity, species relationships, and genome evolution.

No MeSH data available.


Preparation of C0t-1 DNA probes of C. farreri M DL2000 marker; 1 Shearing genomic DNA; 2 C0t-1 DNA; 3 Labeled C0t-1 DNA probes.
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Related In: Results  -  Collection


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fig1: Preparation of C0t-1 DNA probes of C. farreri M DL2000 marker; 1 Shearing genomic DNA; 2 C0t-1 DNA; 3 Labeled C0t-1 DNA probes.

Mentions: The size of the genomic DNA obtained from C. farreri was more than 20 kb (data not shown). After being sonicated, the majority of DNA fragments were within the desired size range of 100–1500 bp. Then by reannealing and S1 nuclease digestion, the final size range of CF C0t-1 DNA is 100–800 bp. The size of CF C0t-1 DNA probes labeled with digoxigenin-11-dUTP by nick translation was ranged from 100 bp to 600 bp (Figure 1).


Comparative Cytogenetics Analysis of Chlamys farreri, Patinopecten yessoensis, and Argopecten irradians with Ct-1 DNA by Fluorescence In Situ Hybridization.

Hu LP, Shang WC, Sun Y, Wang S, Ren XL, Huang XT, Bao ZM - Evid Based Complement Alternat Med (2011)

Preparation of C0t-1 DNA probes of C. farreri M DL2000 marker; 1 Shearing genomic DNA; 2 C0t-1 DNA; 3 Labeled C0t-1 DNA probes.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3138726&req=5

fig1: Preparation of C0t-1 DNA probes of C. farreri M DL2000 marker; 1 Shearing genomic DNA; 2 C0t-1 DNA; 3 Labeled C0t-1 DNA probes.
Mentions: The size of the genomic DNA obtained from C. farreri was more than 20 kb (data not shown). After being sonicated, the majority of DNA fragments were within the desired size range of 100–1500 bp. Then by reannealing and S1 nuclease digestion, the final size range of CF C0t-1 DNA is 100–800 bp. The size of CF C0t-1 DNA probes labeled with digoxigenin-11-dUTP by nick translation was ranged from 100 bp to 600 bp (Figure 1).

Bottom Line: The results showed that C(0)t-1 DNA signals spread on all chromosomes in the three scallops, whereas signal density and intensity were different strikingly.Based on the C(0)t-1 DNA fluorescent bands, a more exact karyotype of C. farreri has been obtained.In this study, the comparative analysis based on C(0)t-1 DNA provides a new insight into the chromosomal reconstructions during the evolution process, and it is helpful for understanding an important source of genomic diversity, species relationships, and genome evolution.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Marine Genetics and Breeding (MGB), College of Marine Life Sciences, Ocean University of China, Ministry of Education, Qingdao 266003, China.

ABSTRACT
The chromosomes of Chlamys farreri, Patinopecten yessoensis, and Argopecten irradians were studied by FISH using C. farreri C(0)t-1 DNA probes. The results showed that C(0)t-1 DNA signals spread on all chromosomes in the three scallops, whereas signal density and intensity were different strikingly. Clustering brighter signals presented in the centromeric and telomeric regions of most C. farreri chromosomes, and in the centromeric or pericentromeric regions of several P. yessoensis chromosomes. Comparative analysis of the mapping indicated a relatively higher homology in the repetitive DNA sequences of the genome between C. farreri and P. yessoensis than that between C. farreri and A. irradians. In addition, FISH showed that the distribution of C(0)t-1 DNA clustering signals in C. farreri displayed completely similar signal bands between homologous chromosomes. Based on the C(0)t-1 DNA fluorescent bands, a more exact karyotype of C. farreri has been obtained. In this study, the comparative analysis based on C(0)t-1 DNA provides a new insight into the chromosomal reconstructions during the evolution process, and it is helpful for understanding an important source of genomic diversity, species relationships, and genome evolution.

No MeSH data available.